Regucalcin increases Ca2+-ATPase activity in the heart mitochondria of normal and regucalcin transgenic rats

Akhter, Tania; Sawada, Natsumi; Yamaguchi, Masayoshi

doi:10.3892/ijmm.18.1.171

Cited by 4 publications

(11 citation statements)

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“…The mitochondrial Ca 2+ -ATPase activity was increased with increasing concentrations of CaCl 2 (2.5–50 μM) (21). An increase in the enzyme activity was saturated at 50 μM CaCl 2 .…”

Section: Regucalcin Regulates Intracellular Ca2+ Homeostasis In Thmentioning

confidence: 99%

“…The role of regucalcin in the regulation of Ca 2+ -ATPase activity in rat heart mitochondria was also demonstrated ( 21 ). The mitochondrial Ca 2+ -ATPase activity was increased with increasing concentrations of CaCl 2 (2.5–50 μM) ( 21 ). An increase in the enzyme activity was saturated at 50 μM CaCl 2 .…”

Section: Regucalcin Regulates Intracellular Ca 2+ mentioning

confidence: 99%

“…An increase in the enzyme activity was saturated at 50 μM CaCl 2 . The addition of regucalcin (10 −11 -10 −8 M) to the enzyme reaction mixture led to an increase in Ca 2+ -ATPase activity in heart mitochondria in the presence of 50 μM CaCl 2 ( 21 ). Regucalcin exerted no effects on mitochondrial Mg 2+ -ATPase activity.…”

Section: Regucalcin Regulates Intracellular Ca 2+ mentioning

confidence: 99%

“…This coupling mechanism is based on the regulation of intracellular Ca 2+ concentration by the Ca 2+ pump in the sarcoplasmic reticulum of heart muscle cells ( 17 – 19 ). Regucalcin is expressed in the heart and was found to increase rat heart sarcoplasmic reticulum Ca 2+ -ATPase (SERCA2a) activity and ATP-dependent Ca 2+ uptake and mitochondrial Ca 2+ -ATPase activity ( 20 , 21 ). Regucalcin was also shown to regulate the activities of various enzymes, which are associated with Ca 2+ signaling in the heart cytoplasm.…”

Section: Introductionmentioning

confidence: 99%

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Regulatory role of regucalcin in heart calcium signaling: Insight into cardiac failure (Review)

Yamaguchi

2014

Biomedical Reports

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Regucalcin was first identified in 1978 as a regulatory protein of Ca2+ signaling in liver cells. Regucalcin was shown to play a multifunctional role in cell regulation, such as maintainance of intracellular Ca2+ homeostasis and suppression of signal transduction, protein synthesis, nuclear function, cell proliferation and apoptosis in various types of cells and tissues. Cardiac excitation-contraction coupling is based on the regulation of intracellular Ca2+ concentration by the Ca2+ pump in the sarcoplasmic reticulum of heart muscle cells. Regucalcin, which is expressed in the heart, was found to increase rat heart sarcoplasmic reticulum Ca2+-ATPase activity and ATP-dependent Ca2+ uptake and mitochondrial Ca2+-ATPase activity. Regucalcin was also shown to suppress Ca2+-dependent protein tyrosine phosphatase, Ca2+/calmodulin-dependent protein phosphatase (calcineurin) and nitric oxide (NO) synthase activity in the heart cytoplasm. Moreover, regucalcin was found to activate superoxide dismutase (SOD), which plays a significant role in the prevention of cell death and apoptosis in the heart. Regucalcin may be a key molecule in heart muscle cell regulation through Ca2+ signaling. Regucalcin may also play a pathophysiological role in heart failure. The aim of this study was to review the recent findings regarding the role of regucalcin in Ca2+ signaling in the heart.

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“…The mitochondrial Ca 2+ -ATPase activity was increased with increasing concentrations of CaCl 2 (2.5–50 μM) (21). An increase in the enzyme activity was saturated at 50 μM CaCl 2 .…”

Section: Regucalcin Regulates Intracellular Ca2+ Homeostasis In Thmentioning

confidence: 99%

Section: Regucalcin Regulates Intracellular Ca 2+ mentioning

confidence: 99%

Section: Regucalcin Regulates Intracellular Ca 2+ mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Regulatory role of regucalcin in heart calcium signaling: Insight into cardiac failure (Review)

Yamaguchi

2014

Biomedical Reports

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“…Recently, it has been shown that regucalcin can activate Ca 2+ -ATPase, which has a role as a Ca 2+ pump, in rat heart microsomes (12) and mitochondria (20), indicating that regucalcin plays a role in the regulation of Ca 2+ homeostasis in heart cells. Moreover, regucalcin has been demonstrated to regulate the activities of nitric oxide synthase (21), protein phosphatase (22), and superoxide dismutase (23), which is related to intracellular signaling, in rat heart cytosol.…”

Section: Introductionmentioning

confidence: 99%

Suppression of regucalcin mRNA expression in the hearts of rats administered with free radical compound: The administration-induced death is accelerated in regucalcin transgenic rats

Akhter

Nakagawa²,

Kobayashi³

et al. 2007

Int J Mol Med

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Abstract. The expression of regucalcin, a regulatory protein in the intracellular signaling system, in the hearts of rats was investigated. Regucalcin expression was examined using reverse transcription-polymerase chain reaction and Western blot analysis. Regucalcin mRNA and its protein levels in the hearts of male and female rats were significantly decreased with increasing age (50 weeks old) as compared with that of 5-week-old rats. The effect of 1,1-diphenyl-2-picryl-hydrazyl (DPPH), a compound that produces free radical, on regucalcin mRNA expression in the hearts of female rats (5 weeks old) was examined. Heart regucalcin mRNA levels were significantly decreased at 60 or 180 min after a single intraperitoneal administration of DPPH (0.5 mg /100 g body weight), suggesting that free radical stress has a suppressive effect on the gene expression. Normal (wild) female rats died at approximately 300 min after a single intraperitoneal administration of DPPH (0.5 mg/100 g), while regucalcin transgenic (TG) female rats died at approximately 150 min after the administration. Heart regucalcin protein in DPPHadministered rats was greater in regucalcin TG rats than in normal (wild) rats. This study demonstrates that the death of regucalcin TG rats is accelerated after the administration of free radical compound, indicating that overexpression of regucalcin does not have effects as the suppressor for free radical stress and the scavenger for free radical in rats.

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Senescence Marker Protein 30 (SMP30) Expression in Eukaryotic Cells: Existence of Multiple Species and Membrane Localization

et al. 2011

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Senescence marker protein (SMP30), also known as regucalcin, is a 34 kDa cytosolic marker protein of aging which plays an important role in intracellular Ca2+ homeostasis, ascorbic acid biosynthesis, oxidative stress, and detoxification of chemical warfare nerve agents. In our goal to investigate the activity of SMP30 for the detoxification of nerve agents, we have produced a recombinant adenovirus expressing human SMP30 as a fusion protein with a hemaglutinin tag (Ad-SMP30-HA). Ad-SMP30-HA transduced the expression of SMP30-HA and two additional forms of SMP30 with molecular sizes ∼28 kDa and 24 kDa in HEK-293A and C3A liver cells in a dose and time-dependent manner. Intravenous administration of Ad-SMP30-HA in mice results in the expression of all the three forms of SMP30 in the liver and diaphragm. LC-MS/MS results confirmed that the lower molecular weight 28 kDa and 24 kDa proteins are related to the 34 kDa SMP30. The 28 kDa and 24 kDa SMP30 forms were also detected in normal rat liver and mice injected with Ad-SMP30-HA suggesting that SMP30 does exist in multiple forms under physiological conditions. Time course experiments in both cell lines suggest that the 28 kDa and 24 kDa SMP30 forms are likely generated from the 34 kDa SMP30. Interestingly, the 28 kDa and 24 kDa SMP30 forms appeared initially in the cytosol and shifted to the particulate fraction. Studies using small molecule inhibitors of proteolytic pathways revealed the potential involvement of β and γ-secretases but not calpains, lysosomal proteases, proteasome and caspases. This is the first report describing the existence of multiple forms of SMP30, their preferential distribution to membranes and their generation through proteolysis possibly mediated by secretase enzymes.

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Regucalcin increases Ca2+-ATPase activity in the heart mitochondria of normal and regucalcin transgenic rats

Cited by 4 publications

References 32 publications

Regulatory role of regucalcin in heart calcium signaling: Insight into cardiac failure (Review)

Regulatory role of regucalcin in heart calcium signaling: Insight into cardiac failure (Review)

Suppression of regucalcin mRNA expression in the hearts of rats administered with free radical compound: The administration-induced death is accelerated in regucalcin transgenic rats

Senescence Marker Protein 30 (SMP30) Expression in Eukaryotic Cells: Existence of Multiple Species and Membrane Localization

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