Regucalcin increases superoxide dismutase activity in the heart cytosol of normal and regucalcin transgenic rats.

Ichikawa, Emiko; Yamaguchi, Masayoshi

doi:10.3892/ijmm.14.4.691

Cited by 13 publications

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“…The role of regucalcin in the regulation of heart muscle function, however, has not been clarified fully. Recent studies demonstrate that regucalcin has activating effects on microsomal Ca 2+ -ATPase (18) and cytosolic superoxide dismutase (26) in rat heart, and that it has inhibitory effects on cytosolic protein phosphatase (27) and nitric oxide synthetase (28) in the heart. This study, moreover, demonstrates that regucalcin has an activating effect on Ca 2+ -ATPase, which is involved in Ca 2+ transport, in the mitochondria of rat heart.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, it has been shown that regucalcin can activate Ca 2+ -ATPase, which plays a role as a Ca 2+ pump, in rat heart microsomes (18), and that it increases superoxide dismutase activity in rat heart cytosol (26). Moreover, regucalcin has been shown to have inhibitory effects on protein phosphatase (27) and nitric oxide synthase (28) activities in rat heart cytosol.…”

Section: Introductionmentioning

confidence: 99%

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Regucalcin increases Ca2+-ATPase activity in the heart mitochondria of normal and regucalcin transgenic rats

Akhter

Sawada²,

Yamaguchi³

2006

Int J Mol Med

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Abstract. The role of regucalcin, a regulatory protein in intracellular signaling system, in the regulation of Ca 2+ -ATPase activity in rat heart mitochondria was investigated. Mitochondrial Ca 2+ -ATPase activity was significantly increased by increasing concentrations of CaCl 2 (2.5-50 μM). An increase in the enzyme activity was saturated at 50 μM CaCl 2 . The addition of regucalcin (10 -11 -10 -8 M) in the enzyme reaction mixture caused a significant increase in Ca 2+ -ATPase activity in heart mitochondria in the presence of 50 μM CaCl 2 . Regucalcin did not have a significant effect on mitochondrial Mg 2+ -ATPase activity. Regucalcin (10 -9 M) did not have a significant effect on Ca 2+ -ATPase activity in the presence of digitonin (10 -3 or 10 -2 %), which is a solubilization effect on membranous lipids. The effect of regucalcin in increasing mitochondrial Ca 2+ -ATPase activity was not observed in the presence of ruthenium red (10 -7 M) or lanthanum chloride (10 -7 M), which is an inhibitor of Ca 2+ uniporter. The effect of regucalcin (10 -9 M) in increasing mitochondrial Ca 2+ -ATPase activity was not significantly enhanced in the presence of calmodulin (5 μg/ml) or dibutyryl cyclic AMP (10 -4 M), which is an intracellular signaling factor that can cause a significant increase in the enzyme activity. Mitochondrial regucalcin localization was significantly increased in the heart of regucalcin transgenic rats as compared with that of normal rats using Western blot analysis. Ca 2+ -ATPase activity was significantly increased in the heart mitochondria of regucalcin transgenic rats. This study demonstrates that regucalcin has an activating effect on Ca 2+ -ATPase in rat heart mitochondria, suggesting its role in the regulation of heart mitochondrial function.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Regucalcin increases Ca2+-ATPase activity in the heart mitochondria of normal and regucalcin transgenic rats

Akhter

Sawada²,

Yamaguchi³

2006

Int J Mol Med

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“…Regucalcin has been shown to have a protective effect against the toxicity of free radical using regucalcin transgenic rats that overexpress regucalcin (23). The time to death of female rats after the administration of DPPH was shortened in regucalcin transgenic rats as compared to normal rats.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, it has been shown that regucalcin can activate Ca 2+ -ATPase, which has a role as a Ca 2+ pump, in rat heart microsomes (12) and mitochondria (20), indicating that regucalcin plays a role in the regulation of Ca 2+ homeostasis in heart cells. Moreover, regucalcin has been demonstrated to regulate the activities of nitric oxide synthase (21), protein phosphatase (22), and superoxide dismutase (23), which is related to intracellular signaling, in rat heart cytosol. Regucalcin may play a role in the regulation of the heart function.…”

Section: Introductionmentioning

confidence: 99%

Suppression of regucalcin mRNA expression in the hearts of rats administered with free radical compound: The administration-induced death is accelerated in regucalcin transgenic rats

Akhter

Nakagawa²,

Kobayashi³

et al. 2007

Int J Mol Med

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Abstract. The expression of regucalcin, a regulatory protein in the intracellular signaling system, in the hearts of rats was investigated. Regucalcin expression was examined using reverse transcription-polymerase chain reaction and Western blot analysis. Regucalcin mRNA and its protein levels in the hearts of male and female rats were significantly decreased with increasing age (50 weeks old) as compared with that of 5-week-old rats. The effect of 1,1-diphenyl-2-picryl-hydrazyl (DPPH), a compound that produces free radical, on regucalcin mRNA expression in the hearts of female rats (5 weeks old) was examined. Heart regucalcin mRNA levels were significantly decreased at 60 or 180 min after a single intraperitoneal administration of DPPH (0.5 mg /100 g body weight), suggesting that free radical stress has a suppressive effect on the gene expression. Normal (wild) female rats died at approximately 300 min after a single intraperitoneal administration of DPPH (0.5 mg/100 g), while regucalcin transgenic (TG) female rats died at approximately 150 min after the administration. Heart regucalcin protein in DPPHadministered rats was greater in regucalcin TG rats than in normal (wild) rats. This study demonstrates that the death of regucalcin TG rats is accelerated after the administration of free radical compound, indicating that overexpression of regucalcin does not have effects as the suppressor for free radical stress and the scavenger for free radical in rats.

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“…Other reports have indicated that SMP30 participates in controlling the activity of antioxidant enzymes (13). SMP30 protein has an activating effect on cytoplasmic superoxide dismutase in liver and heart cells (14,15). SMP30 also functions as an antioxidant protein that has a protective action against oxidative stress without influencing antioxidant enzyme expression (13).…”

Section: Our Experimental Evidence Suggests That Vitamin C Supplementmentioning

confidence: 99%

Preventive Effects of Vitamin C on Diethylnitrosamine-induced Hepatotoxicity in Smp30 Knockout Mice

Son

Ullah

Elfadl

et al. 2018

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Abstract. Vitamin C (L-ascorbic Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death, resulting in approximately 0.7 million deaths each year, and the sixth most common malignancy worldwide (1-3). The recognized risk factors for HCC are hepatic disorder associated with steatosis, cirrhosis, viral hepatitis, alcohol, chemical agents, obesity, metabolic syndrome, and so forth (4). Despite recent advances in diagnosis and treatment, the effectiveness of therapy for HCC remains unsatisfactory, and HCC has a hidden development and a poor prognosis (5, 6). Therefore, there is an urgent requirement for novel therapeutic compounds for the treatment of patients with HCC. Many animal models of hepatocarcinogenesis have been established to examine the effects of therapeutic agents. Diethylnitrosamine (DEN) is frequently used as a hepatocarcinogenic agent in rodents (1). In our experimental model, externally visible foci were observed and measured by stereomicroscopy. It has been established that DEN induces HCC in animals with a developmental course similar to that of human HCC (7). Furthermore, DEN is an indirect alkylating agent that induces the generation of reactive oxygen species (ROS), which damage DNA through oxidation (8).Senescence marker protein-30 (SMP30) is a calciumbinding protein, and its levels in the liver, kidney, and lung become significantly down-regulated with aging in an androgen-independent manner (9, 10). SMP30 plays an important role in calcium (Ca 2+ ) homeostasis (11). Ca 2+ is one of the most universal and physiologically significant signaling molecules that especially affects cell apoptosis and cell necrosis (12). Other reports have indicated that SMP30 participates in controlling the activity of antioxidant enzymes 93

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Regucalcin increases superoxide dismutase activity in the heart cytosol of normal and regucalcin transgenic rats.

Cited by 13 publications

References 0 publications

Regucalcin increases Ca2+-ATPase activity in the heart mitochondria of normal and regucalcin transgenic rats

Regucalcin increases Ca2+-ATPase activity in the heart mitochondria of normal and regucalcin transgenic rats

Suppression of regucalcin mRNA expression in the hearts of rats administered with free radical compound: The administration-induced death is accelerated in regucalcin transgenic rats

Preventive Effects of Vitamin C on Diethylnitrosamine-induced Hepatotoxicity in Smp30 Knockout Mice

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