Regulated formation of eicosanoids

Fitzpatrick, F. A.; Soberman, Roy J.

doi:10.1172/jci13241

Cited by 212 publications

(144 citation statements)

References 38 publications

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“…This finding leads us to suggest that HU releases selectively AA from the inner membrane phospholgycerides (Daak et al, 2011). Our suggestion is consistent with reports that: (i) Prostaglandin E2, the vasodilator metabolite of AA, induces the synthesis of HbF in erythroid colonies derived from peripheral blood cells (Datta, 1985) and the synthesis is obviated by aspirin, the potent inhibitor of cyclooxygenase, COX (Datta et al, 1991); (ii) HU generates NO in vivo (Glover et al, 1999;King, 2004;Nahavandi et al, 2002) and that NO activates cytostolic phospholipase A2 alpha (cPLA2α) and COX2 (Fitzpatrick and Soberman, 2001;Kim et al, 2005;Xu et al, 2008). cPlA2 α has a high selectivity for liberating AA from membrane phosphoglycerides.…”

Section: Hu Treatment and Red Cell Membrane Fatty Acidssupporting

confidence: 90%

Blood Cell Membrane Omega-3 ( n -3) Fatty Acid Abnormality and Supplementation in Patients with Sickle Cell Anemia

Daak

Ghebremeskel

2016

Handbook of Lipids in Human Function

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Section: Hu Treatment and Red Cell Membrane Fatty Acidssupporting

confidence: 90%

Blood Cell Membrane Omega-3 ( n -3) Fatty Acid Abnormality and Supplementation in Patients with Sickle Cell Anemia

Daak

Ghebremeskel

2016

Handbook of Lipids in Human Function

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“…1b). This Fc␥RI-triggered AA generation was almost completely inhibited in cells pretreated with 30 M of methyl arachidonyl fluorophosphate (MAF), an inhibitor of cPLA 2 and iPLA 2 (16), suggesting the participation of cPLA 2 and/or iPLA 2 in the AA generation (Fig. 1b).…”

Section: Resultsmentioning

confidence: 93%

FcγRI-triggered Generation of Arachidonic Acid and Eicosanoids Requires iPLA2 but Not cPLA2 in Human Monocytic Cells

Tay¹,

Melendez

2004

Journal of Biological Chemistry

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Aggregation of receptors for immunoglobulin G (Fc␥Rs) on myeloid cells activates a series of events that are key in the inflammatory response and that can ultimately lead to targeted cell killing by antibody-directed cellular cytotoxicity. Generation of lipid-derived proinflammatory mediators is an important component of the integrated cellular response mediated by receptors for the constant region of immunoglobulins (Fc). We have demonstrated previously that, in interferon-␥-primed U937 cells, the high affinity receptor for IgG, Fc␥RI, is coupled to a novel intracellular signaling pathway that involves the sequential activation of phospholipase D, sphingosine kinase, calcium transients, and protein kinase C isoforms, leading to the activation of the NADPHoxidative burst. Here, we investigate the nature of the phospholipase that regulates arachidonic acid and eicosanoid production. Our data show that Fc␥RI couples to iPLA 2 ␤ for the release of arachidonic acid and the generation of leukotriene B 4 and prostaglandin E 2 . Activation of iPLA 2 ␤ was protein kinase C-dependent; on the other hand, platelet-activating factor triggered cPLA 2 ␣ by means of the mitogen-activated protein kinase pathway. These studies demonstrate that intracellular PLA 2 s can be selectively regulated by different stimuli and suggest a critical role for iPLA 2 ␤ in the intracellular signaling cascades initiated by Fc␥RI and its functional role in the generation of key inflammatory mediators.

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“…Phospholipase A 2 activation releases arachidonic acid (AA,) from membrane phospholipids (Axelrod, 1990;Garcia and Kim, 1997;Nilsson et al, 1998;Pavoine et al, 2003;Vial and Piomelli, 1995). Arachidonic acid and its eicosanoid products are important second messengers that can influence membrane excitability, regional cerebral blood flow (rCBF), gene transcription, mood, and sleep (Chen and Bazan, 2005;Fitzpatrick and Soberman, 2001;Niwa et al, 2000;Rapoport and Bosetti, 2002;Shimizu and Wolfe, 1990;Wang et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

D-Amphetamine Stimulates D₂Dopamine Receptor-Mediated Brain Signaling Involving Arachidonic Acid in Unanesthetized Rats

Bhattacharjee

Chang

White

et al. 2006

J Cereb Blood Flow Metab

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In rat brain, dopaminergic D 2 -like but not D 1 -like receptors can be coupled to phospholipase A 2 (PLA 2 ) activation, to release the second messenger, arachidonic acid (AA, 20:4n-6), from membrane phospholipids. In this study, we hypothesized that D-amphetamine, a dopamine-releasing agent, could initiate such AA signaling. The incorporation coefficient, k* (brain radioactivity/integrated plasma radioactivity) for AA, a marker of the signal, was determined in 62 brain regions of unanesthetized rats that were administered i.p. saline, D-amphetamine (2.5 or 0.5 mg/kg i.p.), or the D 2 -like receptor antagonist raclopride (6 mg/kg, i.v.) before saline or 2.5 mg/kg D-amphetamine. After injecting [1-14 C]AA intravenously, k* was measured by quantitative autoradiography. Compared to saline-treated controls, D-amphetamine 2.5 mg/kg i.p. increased k* significantly in 27 brain areas rich in D 2 -like receptors. Significant increases were evident in neocortical, extrapyramidal, and limbic regions. Pretreatment with raclopride blocked the increments, but raclopride alone did not alter baseline values of k*. In independent experiments, D-amphetamine 0.5 mg/kg i.p. increased k* significantly in only seven regions, including the nucleus accumbens and layer IV neocortical regions. These results indicate that D-amphetamine can indirectly activate brain PLA 2 in the unanesthetized rat, and that activation is initiated entirely at D 2 -like receptors. D-Amphetamine's lowdose effects are consistent with other evidence that the nucleus accumbens, considered a reward center, is particularly sensitive to the drug.

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Regulated formation of eicosanoids

Cited by 212 publications

References 38 publications

Blood Cell Membrane Omega-3 ( n -3) Fatty Acid Abnormality and Supplementation in Patients with Sickle Cell Anemia

Blood Cell Membrane Omega-3 ( n -3) Fatty Acid Abnormality and Supplementation in Patients with Sickle Cell Anemia

FcγRI-triggered Generation of Arachidonic Acid and Eicosanoids Requires iPLA2 but Not cPLA2 in Human Monocytic Cells

D-Amphetamine Stimulates D₂Dopamine Receptor-Mediated Brain Signaling Involving Arachidonic Acid in Unanesthetized Rats

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Regulated formation of eicosanoids

Cited by 212 publications

References 38 publications

Blood Cell Membrane Omega-3 ( n -3) Fatty Acid Abnormality and Supplementation in Patients with Sickle Cell Anemia

Blood Cell Membrane Omega-3 ( n -3) Fatty Acid Abnormality and Supplementation in Patients with Sickle Cell Anemia

FcγRI-triggered Generation of Arachidonic Acid and Eicosanoids Requires iPLA2 but Not cPLA2 in Human Monocytic Cells

D-Amphetamine Stimulates D2Dopamine Receptor-Mediated Brain Signaling Involving Arachidonic Acid in Unanesthetized Rats

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D-Amphetamine Stimulates D₂Dopamine Receptor-Mediated Brain Signaling Involving Arachidonic Acid in Unanesthetized Rats