Regulation and Function of Pilus Island 1 in Group B Streptococcus

Jiang, Sheng-Mei; Park, Su Eun; Yadav, P. S.; Paoletti, Lawrence C.; Wessels, Michael R.

doi:10.1128/jb.00202-12

Cited by 42 publications

(51 citation statements)

References 32 publications

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“…GBS adherence to human epithelial cells. Adherence assays were performed as described previously (13). Briefly, cells were seeded into 24-well tissue culture plates at densities of 5 ϫ 10 5 to 8 ϫ 10 5 per well and cultured at 37°C in 5% CO 2 for 5 days to establish a monolayer.…”

Section: Methodsmentioning

confidence: 99%

“…The GBS strains used in this study were type Ia strain 515; type V strain 2603 (2603V/R); their derivative mutants 515⌬csrR, 2603⌬PI-1, 2603⌬csrR, 2603⌬csrR/⌬PI-1, and 2603⌬csrS; and a repaired ⌬csrR mutant strain, 2603rep (13,14). Unless otherwise specified, GBS bacteria were grown in Todd-Hewitt broth (THB; Difco) or on Trypticase soy agar (TSA) supplemented with 5% defibrinated sheep blood (PML Microbiologicals).…”

Section: Methodsmentioning

confidence: 99%

“…VK2, a human vaginal epithelial cell line; ME180, a human cervical carcinoma cell line; and A549, a human alveolar basal epithelial carcinoma cell line, were cultured as described previously (13). GBS adherence to human epithelial cells.…”

Section: Methodsmentioning

confidence: 99%

“…The GBS cells were collected in the mid-exponential growth phase (A 650 of 0.35) by centrifugation (3,200 ϫ g, 5 min). RNA isolation and qRT-PCR were performed as described previously (13).…”

Section: Methodsmentioning

confidence: 99%

“…Inactivation of CsrRS in GBS strain NEM316 was associated with increased adherence to epithelial cells (19), although the specific adhesins responsible for this phenotype have not been defined. In the course of studying the relationship of pilus function to GBS adherence, we found that CsrR negatively regulates the adherence of GBS strain 2603V/R (referred to here as 2603) to human epithelial cells (13). The chromosome of GBS 2603 harbors two pilus operons, PI-1 and PI-2a.…”

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confidence: 99%

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CsrRS and Environmental pH Regulate Group B Streptococcus Adherence to Human Epithelial Cells and Extracellular Matrix

2012

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Streptococcus agalactiae (group B Streptococcus or GBS) is a common colonizer of the gastrointestinal and genital tracts and an important cause of invasive infections in newborn infants and in adults with predisposing chronic conditions or advanced age. Attachment to epithelial surfaces at mucosal sites is a critical step in the successful colonization of a human host, and regulation of this process is likely to play an important role in both commensalism and dissemination to cause invasive disease. We found that inactivation of the CsrRS (or CovRS) two-component system increased GBS adherence to epithelial cells derived from human vaginal, cervical, and respiratory epithelium, as well as increasing adherence to extracellular matrix proteins and increasing biofilm formation on polystyrene. Neutral (as opposed to acidic) pH enhanced GBS binding to vaginal epithelial cells and to fibrinogen and fibronectin, effects that were partially dependent on CsrRS. The regulatory effects of CsrRS and environmental pH on bacterial adherence correlated with their effects on the expression of multiple surface adhesins, as assessed by quantitative reverse transcription-PCR. We conclude that GBS adherence to epithelial and abiotic surfaces is regulated by the CsrRS twocomponent system and by environmental pH through their regulatory effects on the expression of bacterial surface adhesins. Dynamic regulation of GBS adherence enhances the organism's adaptability to survival in multiple niches in the human host.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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