Regulation and Functionality of a Holin/Endolysin Pair Involved in Killing of Galleria mellonella and Caenorhabditis elegans by Yersinia enterocolitica

Sänger, Philipp-Albert; Knüpfer, Mandy; Kegel, Marcel; Spanier, Britta; Liebler-Tenorio, Elisabeth M.; Fuchs, Thilo M.

doi:10.1128/aem.00036-23

Cited by 3 publications

(1 citation statement)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Whether these and other pathogens make use of specialized lysis-capable cell subpopulations for Tc toxin release in an in vivo setting remains a topic for future research. However, this has already been hinted at by two very recent studies showing that an intact Y. enterocolitica T10SS is as essential for establishing infection in insect and nematode models as is the presence of a functional Y. enterocolitica Tc toxin 58 , 59 . In this light, our finding that an ancient Y. pestis Tc toxin operon from a Black Death victim 60 also encodes a T10SS (Extended Data Fig.…”

Section: Discussionmentioning

confidence: 99%

Yersinia entomophaga Tc toxin is released by T10SS-dependent lysis of specialized cell subpopulations

Sitsel,

Wang,

Janning

et al. 2024

Nat Microbiol

View full text Add to dashboard Cite

Disease-causing bacteria secrete numerous toxins to invade and subjugate their hosts. Unlike many smaller toxins, the secretion machinery of most large toxins remains enigmatic. By combining genomic editing, proteomic profiling and cryo-electron tomography of the insect pathogen Yersinia entomophaga, we demonstrate that a specialized subset of these cells produces a complex toxin cocktail, including the nearly ribosome-sized Tc toxin YenTc, which is subsequently exported by controlled cell lysis using a transcriptionally coupled, pH-dependent type 10 secretion system (T10SS). Our results dissect the Tc toxin export process by a T10SS, identifying that T10SSs operate via a previously unknown lytic mode of action and establishing them as crucial players in the size-insensitive release of cytoplasmically folded toxins. With T10SSs directly embedded in Tc toxin operons of major pathogens, we anticipate that our findings may model an important aspect of pathogenesis in bacteria with substantial impact on agriculture and healthcare.

show abstract

Section: Discussionmentioning

confidence: 99%

Yersinia entomophaga Tc toxin is released by T10SS-dependent lysis of specialized cell subpopulations

Sitsel,

Wang,

Janning

et al. 2024

Nat Microbiol

View full text Add to dashboard Cite

show abstract

Assessment of microbial safety of fresh vegetables through Caenorhabditis elegans model

Mohanapriya,

Paranidharan,

Karthikeyan

et al. 2024

The Microbe

View full text Add to dashboard Cite

Characterization of larval gut microbiota of two endoparasitoid wasps associated with their common host, Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae)

Hu,

Wang,

Zhang

et al. 2024

Microbiol Spectr

View full text Add to dashboard Cite

Insect gut microbes play important roles in digestion, metabolism, development, and environmental adaptation. Parasitoid wasps are one of the most important biological control agents in pest control, while the gut microbial species compositions and the associated functions have been poorly investigated. Two endoparasitoid wasps, Cotesia vestalis and Diadromus collaris , parasitize the larval stage and pupal stage of the diamondback moth, Plutella xylostella , respectively. Using whole-genome shotgun metagenomic sequencing, we characterized the gut microbial composition, diversity, and potential functional roles associated with the two parasitoid wasp larvae. The results reveal that Proteobacteria and Firmicutes are the dominant phyla in the gut of C. vestalis and D. collaris larvae, with Rhizobium and Enterococcus being the dominant genera. The putative microbial functions associated with the two parasitoid wasps might play a virtual role in assisting in consuming the host’s nutritional composition. The enriched CAZymes family genes are primarily involved in the degradation and synthesis of chitin. Despite the richness of microbial species and communities, the microbes species and the microbial community structure exhibit significant similarity between the two parasitoid wasps and between the parasitoid wasp and the host P. xylostella . Notably, the prevalence of the genus Enterococcus shared among them suggests a possible link of gut microbes between the host and their associated parasitoids. Our study offers insights into the gut microbe-based interactions between the host and parasitoid wasps for the first time, potentially paving the way for the development of an ecologically friendly biocontrol strategy against the pest P. xylostella . IMPORTANCE Endoparasitoid wasps spend the majority of their lifespan within their host and heavily rely on the host’s nutrition for survival. There is limited understanding regarding the composition and physiological impacts of gut microbial communities in parasitoid wasps, particularly during the larval stage, which is directly linked to the host. Based on a thorough characterization of the gut microbe and comprehensive comparative analysis, we found the microbial species of the larval parasitoid wasp Cotesia vestalis and the pupal parasitoid wasp Diadromus collaris were similar, sharing 159 genera and 277 species, as were the microbial community structure. Certain of the dominant microbial strains of the two parasitoid wasps were similar to that of their host Plutella xylostella larvae, revealing host insect may affect the microbial community of the parasitoid wasps. The putative microbial functions associated with the parasitoid wasp larvae play an important role in dietary consumption.

show abstract

Regulation and Functionality of a Holin/Endolysin Pair Involved in Killing of Galleria mellonella and Caenorhabditis elegans by Yersinia enterocolitica

Cited by 3 publications

References 62 publications

Yersinia entomophaga Tc toxin is released by T10SS-dependent lysis of specialized cell subpopulations

Yersinia entomophaga Tc toxin is released by T10SS-dependent lysis of specialized cell subpopulations

Assessment of microbial safety of fresh vegetables through Caenorhabditis elegans model

Characterization of larval gut microbiota of two endoparasitoid wasps associated with their common host, Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae)

Contact Info

Product

Resources

About