Regulation by Thyroid Hormone and Retinoic Acid of the CCAAT/Enhancer Binding Protein α and β Genes during Liver Development

Menéndez-Hurtado, Ana; Vega-Núñez, Elena; Pérez-Castillo, Ana

doi:10.1006/bbrc.1997.6635

Cited by 25 publications

(17 citation statements)

References 22 publications

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“…Injection of 15-and 30-day-old hypothyroid animals with thyroid hormone resulted in a slow recovery of hepatic C/EBP-␣ and -␤ mRNA levels. By comparison, retinoic acid was more rapid and potent in stimulating C/EBP-␣ and -␤ expression than thyroid hormone in hypothyroid animals (Menendez-Hurtado et al, 1997).…”

Section: Insulin and Glucocorticoids Regulate Gluconeogenesis Via C/ementioning

confidence: 89%

“…Thyroid Hormone and Retinoic Acid Regulate C/EBP-␣ and -␤ in the Liver. The effect of thyroid hormone and retinoic acid on the expression of C/EBP-␣ and -␤ was investigated in rat liver during development (Menendez-Hurtado et al, 1997). Congenital hypothyroidism caused a significant decrease in both C/EBP-␣ and C/EBP-␤ gene expression at early stages of postnatal development.…”

Section: Insulin and Glucocorticoids Regulate Gluconeogenesis Via C/ementioning

confidence: 99%

“…The discrepancies observed between mRNA and protein levels suggest a translational or post-translational regulation of these genes as the major point of thyroid hormone action on these genes (Menendez-Hurtado et al, 1997).…”

Section: Insulin and Glucocorticoids Regulate Gluconeogenesis Via C/ementioning

confidence: 99%

See 2 more Smart Citations

Liver-Enriched Transcription Factors in Liver Function and Development. Part II: the C/EBPs and D Site-Binding Protein in Cell Cycle Control, Carcinogenesis, Circadian Gene Regulation, Liver Regeneration, Apoptosis, and Liver-Specific Gene Regulation

Schrem

Klempnauer

Borlak

2004

Pharmacological Reviews

193

144

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Section: Insulin and Glucocorticoids Regulate Gluconeogenesis Via C/ementioning

confidence: 89%

Section: Insulin and Glucocorticoids Regulate Gluconeogenesis Via C/ementioning

confidence: 99%

See 1 more Smart Citation

Liver-Enriched Transcription Factors in Liver Function and Development. Part II: the C/EBPs and D Site-Binding Protein in Cell Cycle Control, Carcinogenesis, Circadian Gene Regulation, Liver Regeneration, Apoptosis, and Liver-Specific Gene Regulation

Schrem

Klempnauer

Borlak

2004

Pharmacological Reviews

193

144

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“…Supernatants were removed and stored at Ϫ70°C until analysis by gel electrophoresis. Brain protein extracts were obtained as described previously (28). Twenty g of protein extracts were loaded onto each lane of a 10% SDS-polyacrylamide gel, separated, and then blotted to nitrocellulose membranes at 90 mA overnight in transfer buffer (25 mM Tris, pH 8.3, 190 mM glycine, 20% methanol, 0.5% SDS).…”

Section: Methodsmentioning

confidence: 99%

CCAAT/Enhancer-binding Protein β Plays a Regulatory Role in Differentiation and Apoptosis of Neuroblastoma Cells

Cortes‐Canteli¹,

Pignatelli²,

Santos³

et al. 2002

Journal of Biological Chemistry

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The C/EBP␤ (CCAAT/enhancer-binding protein ␤) is a transcription factor that belongs to basic region-leucine zipper class DNA-binding proteins. There is a significant body of evidence that suggests that this protein plays a central role in adipocytic and eosinophilic differentiation. However, there is no information available regarding the role of this transcription factor in the development of mammalian neuronal tissues. In this study, we have examined the effect of C/EBP␤ overexpression on the differentiation and survival of mouse Neuro2A cells. We found that C/EBP␤ induces neuronal differentiation and that this process is inhibited by transfection with the C/EBP homologous protein 10 (CHOP), strongly suggesting that the extension of neurites is indeed due to the C/EBP␤ transcriptional activity. As it has been suggested in adipocyte differentiation, here we show that C/EBP␤ induces the expression of the endogenous C/EBP␣ gene and that this protein by itself is also able to induce a differentiated phenotype in Neuro2A cells. Neuronal differentiation induced by C/EBP␤ requires activation of the phosphatidylinositol 3-kinase signaling pathway, whereas inhibition of the mitogen-activated protein kinase signaling does not have any effect. In addition, we show that C/EBP␤ is expressed in the brain of neonatal rats, suggesting that this protein could play an important role in neuronal maturation. Finally, cell death was also induced by C/EBP␤ through activation of the p53 protein and the cdk inhibitor p21.

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“…Epimorphin has been shown to promote lumen formation by mammary epithelial cells (Hirai et al, 1998) by increasing the expression of the transcription factor C/EBPβ ) and of MMP . Intriguingly, RA has been reported to enhance the expression of epimorphin (Plateroti et al, 1998), of C/EBPβ (Menéndez-Hurtado et al, 1997;Lee et al, 2002) and of MMP (this study) in epithelial cells. These findings therefore raise the possibility that the lumen-inducing activity of retinoids is mediated in part through the induction of epimorphin.…”

Section: Discussionmentioning

confidence: 54%

Retinoids induce lumen morphogenesis in mammary epithelial cells

Montesano

Soulié

2002

Journal of Cell Science

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Lumen formation is a fundamental step in the development of the structural and functional units of glandular organs, such as alveoli and ducts. In an attempt to elucidate the molecular signals that govern this morphogenetic event, we set up an in vitro system in which cloned mammary epithelial cells grown in collagen gels under serum-free conditions form solid, lumen-less colonies. Addition of as little as 0.1% donor calf serum (DCS) was sufficient to induce the formation of a central cavity. Among a number of serum constituents analyzed, retinol was found to mimic the effect of DCS in inducing lumen morphogenesis. Since the biological activities of retinol are largely dependent on its conversion to all-trans-retinoic acid (RA), we examined in more detail the effect of RA on lumen formation. RA induced the formation of lumen-containing colonies (cysts) in a concentration- and time-dependent manner, a half-maximal effect after 9 days of culture being observed with 100 pM RA. The pleiotropic effects of retinoids are mediated by nuclear retinoic acid receptors (RARs; α, β and γ) and retinoid X receptors (RXRs; α, β and γ). To identify the signaling pathway involved in RA-induced lumen formation, we used receptor-specific synthetic retinoids. TTNPB, a selective RAR agonist,promoted lumen morphogenesis, whereas RXR-selective ligands lacked this activity. Lumen formation was also induced at picomolar concentrations by Am-580, a synthetic retinoid that selectively binds the RARα receptor subtype. Moreover, co-addition of Ro 41-5253, an antagonist of RARα,abrogated the lumen-inducing activity of both RA and DCS, indicating that this biological response is mediated through an RARα-dependent signaling pathway. To gain insight into the mechanisms underlying RA-induced lumen formation, we assessed the potential role of matrix metalloproteinases (MMP). Using gelatin zymography, we observed a dose-dependent increase in latent and active forms of gelatinase B (MMP-9) upon RA treatment. In addition, lumen formation was abrogated by addition of the synthetic MMP inhibitor BB94,indicating that this morphogenetic process is likely to require MMP activity. Collectively, our results provide evidence that RA promotes lumen formation by mammary epithelial cells in vitro and suggest that it plays a similar role during mammary gland development in vivo.

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Regulation by Thyroid Hormone and Retinoic Acid of the CCAAT/Enhancer Binding Protein α and β Genes during Liver Development

Cited by 25 publications

References 22 publications

Liver-Enriched Transcription Factors in Liver Function and Development. Part II: the C/EBPs and D Site-Binding Protein in Cell Cycle Control, Carcinogenesis, Circadian Gene Regulation, Liver Regeneration, Apoptosis, and Liver-Specific Gene Regulation

Liver-Enriched Transcription Factors in Liver Function and Development. Part II: the C/EBPs and D Site-Binding Protein in Cell Cycle Control, Carcinogenesis, Circadian Gene Regulation, Liver Regeneration, Apoptosis, and Liver-Specific Gene Regulation

CCAAT/Enhancer-binding Protein β Plays a Regulatory Role in Differentiation and Apoptosis of Neuroblastoma Cells

Retinoids induce lumen morphogenesis in mammary epithelial cells

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