Regulation of compensatory growth by molecular mechanism in Labeo rohita juveniles under different feeding regimes

Dar, Showkat Ahmad; Srivastava, Prem Prakash; Varghese, Tincy; Rasool, Sheikh Irfan; Anand, Garima; Gupta, Subodh; Gireesh‐Babu, P.; Krishna, Gopal

doi:10.1016/j.ygcen.2018.01.029

Cited by 14 publications

(4 citation statements)

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“…In fact, this compensatory phenomenon has been previously described for other molecular mechanisms. 31,32 The presence of PF-573228 did not affect the Focal adhesion complexes are key structures participating in the interactions between cells and surfaces of biomaterials and may affect cell morphology, proliferation, differentiation, and apoptosis. 35 Vinculin detection has been conducted to identify these complexes, but distinct data have been described [36][37][38] .…”

Section: Effect Of Fak Inhibition On the Gene Expression Of Integrin mentioning

confidence: 93%

Effect of focal adhesion kinase inhibition on osteoblastic cells grown on titanium with different topographies

et al. 2020

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Objective: The present study aimed to investigate the participation of focal adhesion kinases (FAK) in interactions between osteoblastic cells and titanium (Ti) surfaces with three different topographies, namely, untreated (US), microstructured (MS), and nanostructured (NS). Methodology: Osteoblasts harvested from the calvarial bones of 3-day-old rats were cultured on US, MS and NS discs in the presence of PF-573228 (FAK inhibitor) to evaluate osteoblastic differentiation. After 24 h, we evaluated osteoblast morphology and vinculin expression, and on day 10, the following parameters: gene expression of osteoblastic markers and integrin signaling components, FAK protein expression and alkaline phosphatase (ALP) activity. A smooth surface, porosities at the microscale level, and nanocavities were observed in US, MS, and NS, respectively. Results: FAK inhibition decreased the number of filopodia in cells grown on US and MS compared with that in NS. FAK inhibition decreased the gene expression of Alp, bone sialoprotein, osteocalcin, and ALP activity in cells grown on all evaluated surfaces. FAK inhibition did not affect the gene expression of Fak, integrin alpha 1 (Itga1) and integrin beta 1 (Itgb1) in cells grown on MS, increased the gene expression of Fak in cells grown on NS, and increased the gene expression of Itga1 and Itgb1 in cells grown on US and NS. Moreover, FAK protein expression decreased in cells cultured on US but increased in cells cultured on MS and NS after FAK inhibition; no difference in the expression of vinculin was observed among cells grown on all surfaces. Conclusions: Our data demonstrate the relevance of FAK in the interactions between osteoblastic cells and Ti surfaces regardless of surface topography. Nanotopography positively regulated FAK expression and integrin signaling pathway components during osteoblast differentiation.In this context, the development of Ti surfaces with the ability to upregulate FAK activity could positively impact the process of implant osseointegration.

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Section: Effect Of Fak Inhibition On the Gene Expression Of Integrin mentioning

confidence: 93%

Effect of focal adhesion kinase inhibition on osteoblastic cells grown on titanium with different topographies

et al. 2020

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“…Metabolic and/or productive responses under different feeding regimes and involving fasting and refeeding periods have been studied for several species of fish, such as Labeo rohita (Hamilton, 1822) (Dar et al 2018), Oreochromis niloticus (Linnaeus, 1758) (Palma et al 2010), Oncorhynchus mykiss (Walbaum) (Nikki et al 2004) as well as native Brazilian species such as Piaractus mesopotamicus (Holmberg, 1887) (Ortiz et al 2008), C. macropomum (Ituassú et al 2004;Santos et al 2010;Assis et al 2020) and Brycon amazonicus (Spix and Agassiz, 1829) (Urbinati et al 2014). For P. brachypomus juveniles, there are three published studies using short (Favero et al 2022) and/or alternating (Rodríguez and Landines 2011; Rodríguez and Landines-Parra 2018) fasting and refeeding protocols, aiming at compensatory growth.…”

Section: Introductionmentioning

confidence: 99%

Physiological responses to long fasting followed by refeeding in juveniles of pirapitinga, Piaractus brachypomus

PORTO,

ASSIS,

AMORIM

et al. 2023

Acta Amaz.

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For many fish species, prolonged fasting is part of their life cycle, as there are seasonal fluctuations in the quantity and quality of food available in their natural habitat. These animals use endogenous reserves during periods of food scarcity and recover when resources become available again. We evaluated the effect of a prolonged fasting period on indicators of body reserve use, growth performance and intestinal integrity of the Amazonian serrasalmid Piaractus brachypomus. We distributed 66 juveniles (68.6 ± 2.2 g) in 11 tanks. The treatment consisted of 30 days fasting followed by 45 days refeeding and the control of 75 days normal feeding with 5 replicates (one tank with six fish). The six individuals in the 11th tank were used for baseline measurements. Blood parameters, muscle lipid concentration, hepatosomatic and mesenteric fat indices, somatic growth parameters and intestinal villi morphology were measured every 15 days. Glucose, triglycerides, cholesterol, total protein, the mesenteric fat and hepatosomatic indices, weight gain, specific growth rate, condition factor and total biomass decreased significantly during fasting compared to the control, but all except body condition recovered during refeeding. The length and perimeter of the intestinal villi was significantly lower during fasting compared to the control. The feeding protocol allowed P. brachypomus to mobilize part of their body reserves during fasting, however, in general, refeeding was sufficient to restore their body needs and growth performance compatible with that of continuously fed animals.

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“…Many species of fish can mobilize their metabolic and body reserves such as glycogen, lipids, and proteins during a certain period of the year and may go days or even months without feeding due to fluctuations in the demand for food in nature or during the reproduction and spawning seasons when these animals do not feed [1]. However, important factors such as the species and the animals' own physiological needs can influence this mobilization [2]. During refeeding, fish may exhibit hyperphagia, quickly restore their energy needs and body composition, and achieve compensatory growth, corresponding to an animal's ability to grow rapidly after having gone through a period of total or partial food restriction [3,4].…”

Section: Introductionmentioning

confidence: 99%

Effects of Prolonged Fasting and Refeeding on Metabolic, Physiological, Tissue, and Growth Performance Adjustments in Colossoma macropomum

Porto,

Assis,

Amorim

et al. 2024

Fishes

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Fish can tolerate prolonged periods of fasting more easily than endothermic organisms. However, these fasting periods are associated with pronounced lipid and protein catabolism and body weight loss. We evaluated the use of body reserves, growth performance, and the histology of the intestines and muscles of Colossoma macropomum subjected to prolonged fasting for 45 days and refeeding for 14 days. We used 66 juvenile C. macropomum (71.78 ± 10.75 g) distributed in 10 tanks of 100 L in a recirculating aquaculture system (RAS) and kept 6 fish in a separate tank, considered the basal group. The fish were divided into two groups: fed (continuously fed for 59 days) and fasted/refed (subjected to fasting for 45 days and subsequently refed for 14 days). The tambaqui juveniles showed the mobilization of their body reserves during 45 days of fasting but with a large deficit in their growth performance. The 14-day refeeding period was sufficient for fish to restore their energy but insufficient for recovering most growth parameters.

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Regulation of compensatory growth by molecular mechanism in Labeo rohita juveniles under different feeding regimes

Cited by 14 publications

References 56 publications

Effect of focal adhesion kinase inhibition on osteoblastic cells grown on titanium with different topographies

Effect of focal adhesion kinase inhibition on osteoblastic cells grown on titanium with different topographies

Physiological responses to long fasting followed by refeeding in juveniles of pirapitinga, Piaractus brachypomus

Effects of Prolonged Fasting and Refeeding on Metabolic, Physiological, Tissue, and Growth Performance Adjustments in Colossoma macropomum

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