Regulation of Drosophila Hematopoiesis in Lymph Gland: From a Developmental Signaling Point of View

Lan, Wenwen; Liu, Sumin; Zhao, Long; Su, Ying

doi:10.3390/ijms21155246

Cited by 12 publications

(11 citation statements)

References 91 publications

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“…Gcm expression is confined to the haemocytes derived from the first hematopoietic wave that occurs in the procephalic mesoderm of the embryo (19). These haemocytes cease to express Gcm by the end of embryogenesis and survive to the adult where they coexist with those derived from the second wave occurring in the larval lymph gland, which is Gcm independent (59).…”

Section: Resultsmentioning

confidence: 99%

Gcm: a novel anti-inflammatory transcriptional cascade conserved from flies to humans

Pavlidaki

Panic

Monticelli

et al. 2022

Preprint

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Innate immunity is an ancestral process that can induce pro- and anti-inflammatory states. A major challenge is to characterise the transcriptional cascades that modulate the response to chronic and acute inflammatory challenges. The Drosophila melanogaster Gcm transcription factor represents an interesting candidate for its potential anti-inflammatory role. Here we explore its evolutionary conservation and its mode of action. We found that the murine ortholog Gcm2 (mGcm2) is expressed upon aging, which is considered as a state of chronic inflammation. mGcm2 is found in a subpopulation of microglia, the innate immune cells of the central nervous system (CNS). Its expression is also induced by a lyso-phosphatidylcholine (LPC)-induced CNS demyelination (acute inflammation) and mGcm2 conditional knock out mice show an increased inflammatory phenotype upon aging or LPC injection. In agreement with the role of this transcriptional cascade in inflammation, the human ortholog hGCM2 is expressed in active demyelinating lesions of Multiple Sclerosis (MS) patients. Finally, Drosophila gcm expression is induced upon aging as well as during an acute inflammatory response and its overexpression decreases the inflammatory phenotype. Altogether, our data show that the inducible Gcm pathway is highly conserved from flies up to humans and represents a potential therapeutic anti-inflammatory target in the control of the inflammatory response.

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Section: Resultsmentioning

confidence: 99%

Gcm: a novel anti-inflammatory transcriptional cascade conserved from flies to humans

Pavlidaki

Panic

Monticelli

et al. 2022

Preprint

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“…The multi-lobed lymph gland that spans from thoracic to abdominal segment with its stockpile of progenitors at different developmental stages provides us with a unique opportunity to unravel signals dedicated to their maintenance. The extrinsic, intrinsic, and systemic signals critical for maintaining the primary lobe progenitors have been extensively worked out [ 2 , 16 , 59 ]. In comparison, our understanding of the cells in the posterior lobe is limited.…”

Section: Discussionmentioning

confidence: 99%

“…The cells in the cortical zone consist of plasmatocytes, crystal cells, and lamellocytes; the latter formed only upon immune challenge; [ 1 , 15 ]. The primary lobe has been a subject of tremendous investigations revealing several intricate crosstalks between the three distinct zones [ 2 , 7 , 8 , 10 , 16 ]. In contrast, the posterior lobes (comprising secondary and tertiary lobes) of this multi-lobed structure remain comparatively less explored.…”

Section: Introductionmentioning

confidence: 99%

Ubx-Collier signaling cascade maintains blood progenitors in the posterior lobes of the Drosophila larval lymph gland

et al. 2021

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Drosophila larval hematopoiesis occurs in a specialized multi-lobed organ called the lymph gland. Extensive characterization of the organ has provided mechanistic insights into events related to developmental hematopoiesis. Spanning from the thoracic to the abdominal segment of the larvae, this organ comprises a pair of primary, secondary, and tertiary lobes. Much of our understanding arises from the studies on the primary lobe, while the secondary and tertiary lobes have remained mostly unexplored. Previous studies have inferred that these lobes are composed of progenitors that differentiate during pupation; however, the mechanistic basis of this extended progenitor state remains unclear. This study shows that posterior lobe progenitors are maintained by a local signaling center defined by Ubx and Collier in the tertiary lobe. This Ubx zone in the tertiary lobe shares several markers with the niche of the primary lobe. Ubx domain regulates the homeostasis of the posterior lobe progenitors in normal development and an immune-challenged scenario. Our study establishes the lymph gland as a model to tease out how the progenitors interface with the dual niches within an organ during development and disorders.

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“…LG lobes are made up of progenitors that differentiate under specific stress conditions such as wasp infestation (Banerjee et al, 2019;Krzemień et al, 2007;Lan et al, 2020;Lanot et al, 2001;Letourneau et al, 2016). Rodrigues et al (2021) revealed heterogeneity of posterior progenitors and characterized useful genetic tools to further study the population.…”

Section: Drosophila Lymph Glandmentioning

confidence: 99%

“…Whereas previous findings on the LG were associated with the primary lobe, recent studies have elucidated the composition and role of the other pairs of lobes. Posterior LG lobes are made up of progenitors that differentiate under specific stress conditions such as wasp infestation ( Banerjee et al, 2019 ; Krzemień et al, 2007 ; Lan et al, 2020 ; Lanot et al, 2001 ; Letourneau et al, 2016 ). Rodrigues et al (2021) revealed heterogeneity of posterior progenitors and characterized useful genetic tools to further study the population.…”

Section: Drosophila Lymph Glandmentioning

confidence: 99%

Intrinsic and Extrinsic Regulation of Hematopoiesis in Drosophila

Koranteng

Cho

Shim

2022

Molecules and Cells

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Drosophila melanogaster lymph gland, the primary site of hematopoiesis, contains myeloid-like progenitor cells that differentiate into functional hemocytes in the circulation of pupae and adults. Fly hemocytes are dynamic and plastic, and they play diverse roles in the innate immune response and wound healing. Various hematopoietic regulators in the lymph gland ensure the developmental and functional balance between progenitors and mature blood cells. In addition, systemic factors, such as nutrient availability and sensory inputs, integrate environmental variabilities to synchronize the blood development in the lymph gland with larval growth, physiology, and immunity. This review examines the intrinsic and extrinsic factors determining the progenitor states during hemocyte development in the lymph gland and provides new insights for further studies that may extend the frontier of our collective knowledge on hematopoiesis and innate immunity.

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Regulation of Drosophila Hematopoiesis in Lymph Gland: From a Developmental Signaling Point of View

Cited by 12 publications

References 91 publications

Gcm: a novel anti-inflammatory transcriptional cascade conserved from flies to humans

Gcm: a novel anti-inflammatory transcriptional cascade conserved from flies to humans

Ubx-Collier signaling cascade maintains blood progenitors in the posterior lobes of the Drosophila larval lymph gland

Intrinsic and Extrinsic Regulation of Hematopoiesis in Drosophila

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