Regulation of immunogen processing: signal sequences and their application for the new generation of DNA-vaccines

Starodubova, Elizaveta; Isaguliants, Maria; Карпов, В.Л.

doi:10.32607/20758251-2010-2-1-53-59

Cited by 10 publications

(4 citation statements)

References 66 publications

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“…These peptides are generated by the processing of the proteins associated with the lysosomes [35, 36]. Based on these findings, we attempted to fuse our antigen with a natural signal sequence targeting proteins to the lysosome and thus re-direct it into the lysosomal compartment for processing and presentation in complex with MHC class II molecules [7]. …”

Section: Discussionmentioning

confidence: 99%

“…Immunization by genes encoding lysosomal- targeted fusion proteins led to both an enhanced production by B cells of the protective antibodies, and an enhanced cytolytic activity. Also, in most cases, the longevity of the immune response induced by the lysosome- targeted antigen exceeded the longevity of the response to the parental antigen [7]. …”

Section: Discussionmentioning

confidence: 99%

“…The antigenic peptides to be presented on the MHC class II molecules are generated through the lysosomal proteolysis [6]. Antigen presentation by MHC class II molecules can be artificially enhanced by incorporation into the antigen of the lysosomal targeting motives [7]. …”

Section: Introductionmentioning

confidence: 99%

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Fusion to the Lysosome Targeting Signal of the Invariant Chain Alters the Processing and Enhances the Immunogenicity of HIV-1 Reverse Transcriptase

et al. 2014

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Intracellular processing of the antigen encoded by a DNA vaccine is one of the key steps in generating an immune response. Immunization with DNA constructs targeted to the endosomal-lysosomal compartments and to the MHC class II pathway can elicit a strong immune response. Herein, the weakly immunogenic reverse transcriptase of HIV-1 was fused to the minimal lysosomal targeting motif of the human MHC class II invariant chain. The motif fused to the N-terminus shifted the enzyme intracellular localization and accelerated its degradation. Degradation of the chimeric protein occurred predominantly in the lysosomal compartment. BALB/c mice immunized with the plasmid encoding the chimeric protein demonstrated an enhanced immune response, in the form of an increased antigen-specific production of Th1 cytokines, INF-γ and IL-2, by mouse splenocytes. Moreover, the majority of the splenocytes secreted both cytokines; i.e., were polyfunctional. These findings suggest that retargeting of the antigen to the lysosomes enhances the immune response to DNA vaccine candidates with low intrinsic immunogenicity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Fusion to the Lysosome Targeting Signal of the Invariant Chain Alters the Processing and Enhances the Immunogenicity of HIV-1 Reverse Transcriptase

et al. 2014

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“…Clinical trials have also shown that the current generation of DNA vaccines cannot induce a strong antibody response. Therefore, targeting antigen presentation in the MHC-II pathway to activate CD4 + Т cells seems especially advantageous ( 133 ).…”

Section: Vaccines Based On Dcsmentioning

confidence: 99%

New Approaches to Dendritic Cell-Based Therapeutic Vaccines Against HIV-1 Infection

Espinar-Buitrago

Muñoz‐Fernández

2022

Front. Immunol.

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Due to the success of combined antiretroviral therapy (cART) in recent years, the pathological outcome of Human Immunodeficiency Virus type 1 (HIV-1) infection has improved substantially, achieving undetectable viral loads in most cases. Nevertheless, the presence of a viral reservoir formed by latently infected cells results in patients having to maintain treatment for life. In the absence of effective eradication strategies against HIV-1, research efforts are focused on obtaining a cure. One of these approaches is the creation of therapeutic vaccines. In this sense, the most promising one up to now is based on the establishing of the immunological synapse between dendritic cells (DCs) and T lymphocytes (TL). DCs are one of the first cells of the immune system to encounter HIV-1 by acting as antigen presenting cells, bringing about the interaction between innate and adaptive immune responses mediated by TL. Furthermore, TL are the end effector, and their response capacity is essential in the adaptive elimination of cells infected by pathogens. In this review, we summarize the knowledge of the interaction between DCs with TL, as well as the characterization of the specific T-cell response against HIV-1 infection. The use of nanotechnology in the design and improvement of vaccines based on DCs has been researched and presented here with a special emphasis.

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LAMP-1-chimeric DNA vaccines enhance the antibody response in Japanese flounder, Paralichthys olivaceus

Rondón‐Barragán

Nozaki

Hirono

et al. 2017

Fish & Shellfish Immunology

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Regulation of immunogen processing: signal sequences and their application for the new generation of DNA-vaccines

Cited by 10 publications

References 66 publications

Fusion to the Lysosome Targeting Signal of the Invariant Chain Alters the Processing and Enhances the Immunogenicity of HIV-1 Reverse Transcriptase

Fusion to the Lysosome Targeting Signal of the Invariant Chain Alters the Processing and Enhances the Immunogenicity of HIV-1 Reverse Transcriptase

New Approaches to Dendritic Cell-Based Therapeutic Vaccines Against HIV-1 Infection

LAMP-1-chimeric DNA vaccines enhance the antibody response in Japanese flounder, Paralichthys olivaceus

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