Regulation of proliferation and differentiation of adipocyte precursor cells in rainbow trout (Oncorhynchus mykiss)

Bouraoui, Lamia; Gutiérrez, Joaquím; Navarro, Isabel

doi:10.1677/joe-08-0264

Cited by 79 publications

(102 citation statements)

References 67 publications

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“…In these cases, the stimulated TNFα production acts on the adipocyte to shift lipid metabolism from lipid accumulation towards lipid mobilisation (Fonseca-Alaniz et al, 2007;Guilherme et al, 2008;Skurk et al, 2007). The anti-adipogenic and lipolytic effects of TNFα have also been demonstrated in fish (Albalat et al, 2005b;Bouraoui et al, 2008), and interestingly the expression of TNFα is seasonally upregulated in gilthead sea bream with the replenishment of liver and mesenteric fat depots . Thus, in the present study, the lack of changes in TNFα expression with the increase in MFI suggests that fat fish have not reached their peak of fat storage capacity and continue increasing the size of their body fat depots.…”

Section: Discussionmentioning

confidence: 99%

“…This proinflammatory cytokine affects many aspects of adipocyte function, and its lipolytic action has been demonstrated in rainbow trout and gilthead sea bream adipocytes (Albalat et al, 2005b;. Recent studies have also shown that TNFα inhibits the differentiation of rainbow trout preadipocytes (Bouraoui et al, 2008). This, together with the high expression level of TNFα in the fat storage organs of gilthead sea bream , makes this cytokine a good candidate for playing a key role in reducing the adipose tissue mass.…”

Section: Introductionmentioning

confidence: 99%

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Targets for TNFα-induced lipolysis in gilthead sea bream(Sparus aurata L.) adipocytes isolated from lean and fat juvenile fish

Cruz-García

Saera-Vila

Navarro

et al. 2009

Journal of Experimental Biology

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SUMMARYThe present study aimed to analyze adiposity heterogeneity and the role of liver X receptor (LXRα) and peroxisome proliferatoractivated receptors (PPARs) as targets of tumour necrosis factor-α (TNFα) in gilthead sea bream (Sparus aurata L.). The screening of 20 fish at the beginning of the warm season identified two major groups with fat and lean phenotypes. Fat fish showed increased liver and mesenteric fat depots. This increased adiposity was concurrent in the adipose tissue to enhanced expression of lipoprotein lipase (LPL) whereas mRNA levels of the hormone-sensitive lipase (HSL) remained almost unchanged. The resulting LPL/HSL ratio was thereby highest in fat fish, which suggests that this group of fish has not reached its peak fat storage capacity. This is not surprising given the increased expression of PPARγ in the absence of a counter-regulatory raise of TNFα. However, this lipolytic cytokine exerted dual effects in primary adipocyte cultures that differ within and between lean and fat fish. One set of fat fish did not respond to TNFα treatment whereas a second set exhibited a lipolytic response (increased glycerol release) that was apparently mediated by the downregulated expression of PPARβ. In lean fish, TNFα exerted a strong and nontranscriptionally mediated lipolytic action. Alternatively, TNFα would inhibit lipid deposition via the downregulated expression of adipogenic nuclear factors (PPARγ and LXRα). TNFα targets are therefore different in fish with lean and fat phenotypes, which is indicative of the complex network involved in the regulation of fish lipid metabolism.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Targets for TNFα-induced lipolysis in gilthead sea bream(Sparus aurata L.) adipocytes isolated from lean and fat juvenile fish

Cruz-García

Saera-Vila

Navarro

et al. 2009

Journal of Experimental Biology

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“…Preadipocyte cell culture was carried out and differentiated in vitro as previously reported (Bouraoui et al, 2008). Samples for RNA extraction were taken at different development stages of myocytes [days2 (myocytes), 6 (myoblasts) and 11 (myotubes) of culture] and pre-adipocytes [days8 (when the differentiation cocktail was added to the cell medium), 15 (early differentiated adipocytes) and 22 (fully differentiated adipocytes) of culture].…”

Section: Cell Culture Proceduresmentioning

confidence: 99%

Adiponectin effects and gene expression in rainbow trout: an in vivo and in vitro approach

Sanchez-Gurmaches

Cruz-García

Gutiérrez

et al. 2012

Journal of Experimental Biology

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SUMMARYHere we present the presence of adiponectin and adiponectin receptors [type 1 (adipoR1) and type 2 (adipoR2)] in rainbow trout (Oncorhynchus mykiss) tissues and cell cultures together with the response to different scenarios. In response to fasting, adiponectin expression was up-regulated in adipose tissue, while the expression of its receptors increased in white and red muscle. Insulin injection decreased adipoR1 expression in white and red muscles. We deduce that the adipoRs in trout muscle show opposite responses to increasing insulin plasma levels, which may maintain sensitivity to insulin in this tissue. Adiponectin expression was inhibited by the inflammatory effect of lipopolysaccharide (LPS) in adipose tissue and red muscle. Moreover, results indicate that LPS may lead to mobilization of fat reserves, increasing adipoR1 expression in adipose tissue. The effects of LPS could be mediated through tumour necrosis factor  (TNF), at least in red muscle. Insulin, growth hormone and TNF all diminished expression of adipoR2 in adipocytes and adipoR1 in myotubes, while insulin increased the expression of adipoR2 in the muscle cells. Adiponectin activates Akt in rainbow trout myotubes, which may lead to an increase in fatty acid uptake and oxidation. Overall, our results show that the adiponectin system responds differently to various physiological challenges and that it is hormonally controlled in vivo and in vitro. To the best of our knowledge, this is the first time this has been demonstrated in teleosts, and it may be a valuable contribution to our understanding of adipokines in fish.

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“…These transcription factors play key roles in initiating the differentiation of preadipocytes [32]. The C/EBPα and PPARγ genes encode transcription factors that are required for the in vivo differentiation of preadipocytes into adipocytes, and these factors fulfill critical functions during the early stages of adipocyte formation and in the terminal differentiation of adipocytes [33,34]. PPARγ not only induces C/EBPα but also increases its own level of expression; similarly, C/EBPα not only induces the expression of PPARγ but also increases its own level of expression.…”

Section: Discussionmentioning

confidence: 99%

The MBD4 Gene Plays an Important Role in Porcine Adipocyte Differentiation

Zhang

Zhu

Gao

et al. 2014

Cell Physiol Biochem

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Background: MBD4 (methyl-CpG binding domain protein 4) is an important G: T glycosylase that can identify T-G mismatches. It plays a role in active demethylation through base excision repair. Overexpression of MBD4 gene can cause the demethylation of numerous genes, and the remethylation of MBD4-associated genes can occur when the MBD4 gene is knocked out. To date, the functions and regulatory mechanisms of the MBD4 gene in the differentiation of porcine preadipocytes have not been clearly established. Methods: Subcutaneous fat cells from 1- to 7-day-old Junmu-1 piglets were cultured in vitro, induced to differentiate, and then identified. A real-time fluorescence-based quantitative polymerase chain reaction (PCR) analysis was conducted to detect MBD4 messenger RNA (mRNA) expression. Cells were treated with MBD4-siRNA (small interfering RNA) and induced to differentiate. Changes in the lipid droplets were observed by oil red O staining. Changes in the mRNA and protein expression levels of MBD4 and the adipose differentiation-associated genes C/EBPα (CCAAT-enhancer-binding protein alpha), PPARγ (peroxisome proliferator-activated receptor gamma), and aP2 (adipocyte protein 2) were detected. In addition, the bisulfite sequencing method was used to detect changes in methylation in the promoters of certain genes associated with adipose differentiation. Results: Levels of MBD4 mRNA and protein expression varied with time over the course of the porcine adipocyte differentiation, with the highest levels of this expression observed on day two of the differentiation process. After silencing MBD4 and inducing differentiation, the production of lipid droplets decreased, the mRNA expression levels of C/EBPα, PPARγ, and aP2 were significantly reduced, and DNA methylation modification levels were significantly elevated in the examined promoter regions. Conclusion: The silencing of the MBD4 gene can influence the DNA methylation levels of preadipocyte differentiation-related genes and subsequently inhibit the differentiation of porcine preadipocytes.

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Regulation of proliferation and differentiation of adipocyte precursor cells in rainbow trout (Oncorhynchus mykiss)

Cited by 79 publications

References 67 publications

Targets for TNFα-induced lipolysis in gilthead sea bream(Sparus aurata L.) adipocytes isolated from lean and fat juvenile fish

Targets for TNFα-induced lipolysis in gilthead sea bream(Sparus aurata L.) adipocytes isolated from lean and fat juvenile fish

Adiponectin effects and gene expression in rainbow trout: an in vivo and in vitro approach

The MBD4 Gene Plays an Important Role in Porcine Adipocyte Differentiation

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