Regulation of the extrinsic and intrinsic apoptotic pathways in the prefrontal cortex of short- and long-term human opiate abusers

García-Fuster, M. Julia; Ramos-Miguel, Alfredo; Rivero, Guadalupe; Harpe, R. La; Meana, J. Javier; García-Sevilla, J.A.

doi:10.1016/j.neuroscience.2008.09.002

Cited by 46 publications

(33 citation statements)

References 70 publications

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“…Active caspase-9 then cleaves and activates pro-caspase-3 to initiate a cascade of additional caspase activation, culminating in apoptosis. Thus, ART-induced apoptosis of HOS cells may be closely correlated with the intrinsic pathway, which is regulated mainly by Bcl-2, Bax, and cytochrome c (Garcia-Fuster et al, 2008). And this mechanism was also observed in doxorubicin-resistant T leukemia cells by Efferth et al (2007).…”

Section: Discussionmentioning

confidence: 79%

Artesunate inhibits growth and induces apoptosis in human osteosarcoma HOS cell line in vitro and in vivo

Peng

et al. 2011

J. Zhejiang Univ. Sci. B

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This paper aims to investigate the effects of artesunate (ART) on growth and apoptosis in human osteosarcoma HOS cell line in vitro and in vivo and to explore the possible underlying mechanisms. Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The induction of apoptosis was detected by light and transmission electron microscopy and flow cytometry. Western blot analysis was used to investigate the related mechanisms. Nude mice were further employed to investigate the antitumour activity of ART in vivo. MTT assay results demonstrated that ART selectively inhibits the growth of HOS cells in a dose-and time-dependent manner. Based on the findings of light and transmission electron microscopy, Hoechst 33258 staining, and fluorescein isothiocyanate (FITC)-annexin V staining, the cytotoxicity of ART in HOS cells occurs through apoptosis. With ART treatment, cytosolic cytochrome c was increased, Bax expression was gradually upregulated, Bcl-2 expression was downregulated, and caspase-9 and caspase-3 were activated. Thus, the intrinsic apoptotic pathway may be involved in ART-induced apoptosis. Cell cycle analysis by flow cytometry indicated that ART may induce cell cycle arrest at G 2 /M phase. In nude mice bearing HOS xenograft tumours, ART inhibited tumour growth and regulated the expressions of cleaved caspase-3 and survivin, in agreement with in vitro observations. ART has a selective antitumour activity against human osteosarcoma HOS cells, which may be related to its effects on induction of apoptosis via the intrinsic pathway. The results suggest that ART is a promising candidate for the treatment of osteosarcoma.

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Section: Discussionmentioning

confidence: 79%

Artesunate inhibits growth and induces apoptosis in human osteosarcoma HOS cell line in vitro and in vivo

Peng

et al. 2011

J. Zhejiang Univ. Sci. B

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“…In the present study, we found that asterosaponin 1, a new cytostatic agent from the starfish, inhibited the proliferation of human lung cancer A549 cells in a dose-dependent manner, and the cytostatic activity resulted from the induction of cell apoptosis. Apoptosis can be initiated by several distinct, but interconnected, molecular signaling pathways, among which the extrinsic (mediated by the activation of death receptors) and the intrinsic (mitochondria-dependent signaling) pathways represent the two major well-studied apoptotic processes (22). In the past few decades, ER stress, a more recently discovered Figure 5.…”

Section: Discussionmentioning

confidence: 99%

Asterosaponin 1 induces endoplasmic reticulum stress-associated apoptosis in A549 human lung cancer cells

Zhao

Zhu²,

Li³

et al. 2011

Oncol Rep

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Abstract. Asterosaponin 1, a new cytostatic agent from starfish, possesses several bioactivities including an antitumor effect. The aim of this study was to investigate the potential anti-proliferative and pro-apoptotic activity of asterosaponin 1 in A549 human lung cancer cells, as well as the potential mechanisms. The results showed that asterosaponin 1 inhibited the proliferation of A549 cells in a dose-dependent manner, and the cytostatic activity resulted from the induction of apoptotic cell death. Asterosaponin 1 increased ER dilation and cytosolic Ca 2+ concentration, and enhanced the expression of the ER molecular chaperones GRP78 and GRP94 in a doseand time-dependent manner. In addition, asterosaponin 1 treated A549 cells exerted increased expression and activity of CHOP, caspase-4 and JNK, three essential ER-associated apoptotic molecules. In summary, these results demonstrated that asterosaponin 1 inhibits the proliferation of A549 cells through induction of ER stress-associated apoptosis, making asterosaponin 1 a candidate new anticancer drug for lung cancer therapy.

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“…Besides the role of FADD in the cascades of apoptotic signaling in drug addiction (García-Fuster et al, 2007a, 2008bRamos-Miguel et al, 2009;Álvaro-Bartolomé et al, 2011), several pathways have been postulated to link FADD with some forms of behavioral plasticity induced by drugs of abuse, especially heroin/morphine (Ramos-Miguel et al, 2009, 2011 and cocaine , 2011Álvaro-Bartolomé et al, 2011) (Fig. 3).…”

Section: Fadd Adaptor: Apoptotic and Non-apoptotic Signalling Pathwaysmentioning

confidence: 99%

“…In fact, morphine was reported to increase, through a naloxone-sensitive mechanism, the expression of Fas receptor mRNA in mouse splenocytes and in human blood lymphocytes (Yin et al, 1999). However, the possibility of opiate-induced cell death in the mature brain, including the brains of human addicts, still is a debated issue (Boronat et al, 2001;Tegeder and Geisslinger, 2004;Liao et al, 2005;CunhaOliveira et al, 2008;García-Fuster et al, 2008b;Tramullas et al, 2008;Zhang et al, 2008). Fig.…”

Section: Role Of Fadd Adaptor In Opiate Addictionmentioning

confidence: 99%

“…Recent studies have investigated the role of Fas receptor, FADD adaptor and its phosphorylation, other pro-and anti-apoptotic proteins, and FADD-associated signalling pathways, in postmortem brains of long-term opiate addicts (García-Fuster et al, 2008b;Ramos-Miguel et al, 2009). The prefrontal cortex (Brodmann's area 9, middle frontal gyrus; PFC/BA9) was the region selected for examination because it is directly related with the mesocorticolimbic dopaminergic system and the rewarding and addictive properties of opiates and other drugs of abuse.…”

Section: Regulation Of Apoptotic Pathways and Associated Signalling Imentioning

confidence: 99%

See 1 more Smart Citation

Role of Multifunctional FADD (Fas-Associated Death Domain) Adaptor in Drug Addiction

Ramos-Miguel¹,

Álvaro-Bartolomé²,

García-Fuster³

et al. 2012

Addictions - From Pathophysiology to Treatment

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Regulation of the extrinsic and intrinsic apoptotic pathways in the prefrontal cortex of short- and long-term human opiate abusers

Cited by 46 publications

References 70 publications

Artesunate inhibits growth and induces apoptosis in human osteosarcoma HOS cell line in vitro and in vivo

Artesunate inhibits growth and induces apoptosis in human osteosarcoma HOS cell line in vitro and in vivo

Asterosaponin 1 induces endoplasmic reticulum stress-associated apoptosis in A549 human lung cancer cells

Role of Multifunctional FADD (Fas-Associated Death Domain) Adaptor in Drug Addiction

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