Regulatory Sequences Involved in the Promotion and Termination of Rna Transcription

Rosenberg, Martin; Court, Donald CourtD.

doi:10.1146/annurev.ge.13.120179.001535

Cited by 2,719 publications

(1,529 citation statements)

References 57 publications

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“…The exact role of such structures is unknown at present. Upstream of this region, there is a possible Pribnow-box (-10 region) -'5dgAgAATG-148 (lower case letters denote nucleotides which do not share homology with the consensus sequence) and the RNA polymerase recognition site (-35 region) -179TGTTGgattaTT-168, both of which have good homology with the consensus sequences [23].…”

Section: Discussionmentioning

confidence: 99%

DNA sequence of the promoter region of the ompC gene and the amino acid sequence of the signal peptide of pro‐OmpC protein in Escherichia coli

Mizuno¹,

Chou²,

Inouye³

1983

FEBS Letters

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The osmoregulated ompC gene of Escherichia coli was cloned and the DNA sequence of a fragment encompassing the promoter region and a portion of the coding region was determined. There were no obvious homologies in the DNA sequence of the promoter regions of the ompC and ompF genes, in contrast to those of the coding regions of the two genes, both of which code for the matrix porins (major outer membrane proteins) and form passive diffusion pores. The amino acid sequence of the signal peptide of pro‐OmpC protein was also deduced from the DNA sequence

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Section: Discussionmentioning

confidence: 99%

DNA sequence of the promoter region of the ompC gene and the amino acid sequence of the signal peptide of pro‐OmpC protein in Escherichia coli

Mizuno¹,

Chou²,

Inouye³

1983

FEBS Letters

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“…Figure 3 reveals that transcription initiates at nucleotide position -36, and is preceded by potential -10 and -35 promoter sites (Fig. l) homologous to the consensus elements of E. coli promoters (23,50).…”

Section: The Fabb Genementioning

confidence: 99%

“…An inverted repeat (nucleotides 1297-1324, Fig. l) further downstream may function as a rho-independent terminator (47,50). Whether it plays a role infabB transcription is unknown.…”

Section: The Fabb Genementioning

confidence: 99%

β-ketoacyl-ACP synthase I of Escherichia coli: Nucleotide sequence of thefabB gene and identification of the cerulenin binding residue

Kauppinen

Siggaard-Andersen

1988

Carlsberg Res. Commun.

159

124

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Cephalosporium caerulens, polyketideThe fabB gene of E. coli encoding ~-ketoacyl-ACP synthase I has been isolated by complementation and sequenced. The enzyme has been purified and its NH2-terminal residues sequenced. Identification of the active site was accomplished by tagging with 3H-cerulenin and radio sequencing of the region. Comparison of the deduced primary structures of thefabB gene product with the FAS2 gene product of Saccharomyces cerevisiae revealed the probable active site in chalcone synthases of higher plants.

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“…The putative initiation codon ATG was preceded at 6 bp upstream by a potential ribosome-binding sequence (5 0 -AGGAGG-3 0 ) that is homologous to the consensus Shine-Dalgarno sequence.19) The sequences TTGTAT and TATAAT, with a 15-bp spacing, showing certain homology to the consensus promoter sequences of À35 and À10 regions for E. coli 70 factor, TTGACA and TATAAT with a 17-bp spacing, respectively, were identified upstream of the coding region. 20) Amino acid sequence of Xyl43B The deduced protein sequence of Xyl43B did not contain a typical signal peptide sequence conserved in bacterial extracellular proteins, 21) suggesting that Xyn43B is an intracellular enzyme. The deduced protein sequence of Xyl43B was compared with entries in the DDBJ database.…”

Section: Resultsmentioning

confidence: 99%

Sequencing and Expression of the Gene Encoding theClostridium stercorariumβ-Xylosidase Xyl43B inEscherichia coli

Suryani

Kimura

Sakka

et al. 2004

Bioscience, Biotechnology, and Biochemistry

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The Clostridium stercorarium F-9 xyl43B gene encoding the -xylosidase Xyl43B consists of an open reading frame of 1,491 nucleotides that encodes a putative protein, classified in family 43, of 497 amino acids with a predicted molecular weight of 56,355. The deduced amino acid sequence of Xyl43B has sequence similarity with -xylosidases from Bacteriodes thetaiotaomicron (57% sequence identity), Prevotella ruminicola (45%), Streptomyces coelicolor (40%), and Clostridium acetobutylicum (36%), all of which have been classified in family 43 of the glycoside hydrolases. Xyl43B was purified from a recombinant Escherichia coli and characterized. The optimum pH of the purified enzyme was 3.5 and it was stable over pH from 3.0 to 8.0. Its optimum temperature was 80 C and it showed thermostability in the temperature range from 50 to 70 C. Xyl43B had a K m of 6.2 mM and a V max of 15 mol min À1 mg À1 for p-nitrophenyl--D-xylopyranoside.

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Regulatory Sequences Involved in the Promotion and Termination of Rna Transcription

Cited by 2,719 publications

References 57 publications

DNA sequence of the promoter region of the ompC gene and the amino acid sequence of the signal peptide of pro‐OmpC protein in Escherichia coli

DNA sequence of the promoter region of the ompC gene and the amino acid sequence of the signal peptide of pro‐OmpC protein in Escherichia coli

β-ketoacyl-ACP synthase I of Escherichia coli: Nucleotide sequence of thefabB gene and identification of the cerulenin binding residue

Sequencing and Expression of the Gene Encoding theClostridium stercorariumβ-Xylosidase Xyl43B inEscherichia coli

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