Regulatory T Cell Depletion Abolishes the Protective Effect of Dietary Galacto-Oligosaccharides on Eosinophilic Airway Inflammation in House Dust Mite–Induced Asthma in Mice

Verheijden, Kim A.T.; Braber, Saskia; Leusink-Muis, Thea; Thijssen, Suzan; Boon, Louis; Kraneveld, Aletta D.; Garssen, Johan; Folkerts, Gert; Willemsen, Linette E. M.

doi:10.3945/jn.115.224402

Cited by 17 publications

(11 citation statements)

References 46 publications

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“…After 2'FL and 6'SL treatment during challenge in ovalbumin sensitized mice enhanced the capacity of CD4+CD25+ T reg cells to inhibit mast cell degranulation ex vivo (39), indicating that specific sHMOS support T reg cell function. Similar results were found using scGOS and lcFOS in combination with acidic oligosaccharides or B. breve in prevention of food- (29,166) or asthma-allergy in mice (31,165). In piglets, either sow-reared or formula fed, peripheral blood mononuclear cells (PBMCs) were isolated (164).…”

Section: Hmos Interact With Immune Cellssupporting

confidence: 62%

Immunomodulation by Human Milk Oligosaccharides: The Potential Role in Prevention of Allergic Diseases

et al. 2020

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Section: Hmos Interact With Immune Cellssupporting

confidence: 62%

Immunomodulation by Human Milk Oligosaccharides: The Potential Role in Prevention of Allergic Diseases

et al. 2020

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“…HDM sensitization and challenge did not affect the frequency of Treg and budesonide treatment tended to lower the %Treg which could not be rescued by the dietary intervention with GOS. However, in a previous study GOS was shown to improve the regulatory function of the CD25+ cells, which include Treg and via this way GOS may have supported the anti-inflammatory capacities of budesonide treatment ( 68 ).…”

Section: Discussionmentioning

confidence: 97%

The Combination Therapy of Dietary Galacto-Oligosaccharides With Budesonide Reduces Pulmonary Th2 Driving Mediators and Mast Cell Degranulation in a Murine Model of House Dust Mite Induced Asthma

et al. 2018

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Background: Dietary non-digestible galacto-oligosaccharides (GOS) suppress allergic responses in mice sensitized and challenged with house dust mite (HDM). Budesonide is the standard therapy for allergic asthma in humans but is not always completely effective.Aim: To compare the efficacy of budesonide or different doses of GOS alone or with a combination therapy of budesonide and GOS on HDM-allergic responses in mice.Methods:BALB/c mice were sensitized and challenged with HDM, while fed a control diet or a diet supplemented with 1 or 2.5 w/w% GOS, and either or not oropharyngeally instilled with budesonide. Systemic and local inflammatory markers, such as mucosal mast cell protease-1 (mMCP-1) in serum, pulmonary CCL17, CCL22, and IL-33 concentrations and inflammatory cell influx in the bronchoalveolar lavage fluid (BALF) were determined.Results: Budesonide or GOS alone suppressed the number of eosinophils in the BALF of HDM allergic mice whereas budesonide either or not combined with GOS lowered both eosinophil and lymphocyte numbers in the BALF of HDM-allergic mice. Both 1 w/w% and 2.5 w/w% GOS and/or budesonide suppressed serum mMCP-1 concentrations. However, budesonide nor GOS alone was capable of reducing Th2 driving chemokines CCL17, CCL22 and IL-33 protein levels in supernatants of lung homogenates of HDM allergic mice, whereas the combination therapy did. Moreover, IL-13 concentrations were only significantly suppressed in mice treated with budesonide while fed GOS. A similar tendency was observed for the frequency of GATA3+CD4+ Th2 and CD4+RORγt+ Th17 cells in the lungs of the allergic mice.Conclusion: Dietary intervention using GOS may be a novel way to further improve the efficacy of anti-inflammatory drug therapy in allergic asthma by lowering Th2 driving mediators and mast cell degranulation.

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“…While there is evidence that supplementing diets with certain nondigestible oligosaccharides can reduce the incidence of allergic disease in children (Gruber et al., ; Moro et al., ) and attenuate symptoms of allergy in animal models (Castillo‐Courtade et al., ; Hogenkamp, Knippels, Garssen, & van Esch, ; Schouten et al., ; Verheijden et al., , ) the mechanisms of action have yet to be fully elucidated.…”

Section: Discussionmentioning

confidence: 99%

Human Milk Oligosaccharides Attenuate Antigen–Antibody Complex Induced Chemokine Release from Human Intestinal Epithelial Cell Lines

Zehra

Khambati

Vierhout

et al. 2018

Journal of Food Science

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This study provides evidence for direct effects of HMOs in addition to their prebiotic role and demonstrates, for the first time, modulation of Ag-IgE complex activation of human epithelial cells that may have important implications for food-allergy. The study also reinforces the concept that structurally different oligosaccharides have distinct biological activities. In determining the composition of infant formula, addition of oligosaccharides with specific structures may provide direct modulation of immune responses and potentially attenuate symptoms or development of food allergy.

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Regulatory T Cell Depletion Abolishes the Protective Effect of Dietary Galacto-Oligosaccharides on Eosinophilic Airway Inflammation in House Dust Mite–Induced Asthma in Mice

Cited by 17 publications

References 46 publications

Immunomodulation by Human Milk Oligosaccharides: The Potential Role in Prevention of Allergic Diseases

Immunomodulation by Human Milk Oligosaccharides: The Potential Role in Prevention of Allergic Diseases

The Combination Therapy of Dietary Galacto-Oligosaccharides With Budesonide Reduces Pulmonary Th2 Driving Mediators and Mast Cell Degranulation in a Murine Model of House Dust Mite Induced Asthma

Human Milk Oligosaccharides Attenuate Antigen–Antibody Complex Induced Chemokine Release from Human Intestinal Epithelial Cell Lines

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