Rehaibacterium terrae gen. nov., sp. nov. isolated from a geothermally heated soil sample

Abstract

A thermotolerant, alkalitolerant, Gram-stain-negative and strictly aerobic bacterium, designated strain YIM 77974 T , was isolated from a geothermally heated soil sample collected at Rehai National Park, Tengchong, Yunnan province, south-west China. Cells of the strain were rodshaped and colonies were light brown and circular. The strain grew in the presence of 0-3 % (w/v) NaCl (optimum, 0-1 %) and at pH 7.0-10.0 (optimum, pH 8.0) and 30-55 6C (optimum, 45 6C). The only quinone was Q-8 and the genomic DNA G+C content was 68.3 mol%. Major fatty acids (.10 %) were iso-C 16 : 0 , iso-C 17 : 0 , iso-C 15 : 0 and iso-C 11 : 0 . The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, an unidentified aminophospholipid, three unidentified phospholipids and two unidentified polar lipids. On the basis of the morphological and chemotaxonomic characteristics as well as genotypic data, it is proposed that this strain should be classified as a representative of a novel genus and species, Rehaibacterium terrae gen. nov., sp. nov., in the family Xanthomonadaceae. The type strain is strain YIM 77974 T (5DSM 25897 T 5CCTCC AB 2012062 T ).The family Xanthomonadaceae belongs to the class Gammaproteobacteria and comprises 22 genera at the time of writing (http://www.bacterio.net/classifgenerafamilies.html). The genus Silanimonas was established by Lee et al. (2005) and it comprises two species with validly published names, Silanimonas lenta (type strain DSM 16282 T ) and Silanimonas mangrovi (type strain AK13 T ) (Srinivas et al., 2012). During investigations of the thermophilic microbial resources at Rehai National Park, a novel thermotolerant strain, YIM 77974 T , was isolated. Phylogenetic trees analysis based on 16S rRNA gene sequences revealed that the strain YIM 77974 T was closely related to the species of the genus Silanimonas in the family Xanthomonadaceae. However it is clearly distinct from the members of the genus Silanimonas by high sequence divergence values and the significant differences of morphological and chemotaxonomic characteristics. In the present study, we report the results of a polyphasic taxonomic study of this thermotolerant strain YIM 77974 T .Strain YIM 77974 T was isolated from a geothermally heated soil sample collected from Rehai National Park, Tengchong, Yunnan Province, south-west China (24.95002 u N 98.43742 u W), by using serial dilution. The geothermally heated soil sample was sandy, and the temperature and pH were 53 u C and 7.5, respectively. After 1 week of incubation on R2A agar medium (BD; Becton, Dickinson and Company) at 45 u C, colonies were picked and repeatedly restreaked onto R2A agar medium at 45 uC, until purity was confirmed. The purified strains were routinely cultivated and stored as aqueous glycerol suspensions (20 %, v/v) at 280 u C.Cell morphology was determined with cultures grown for 6, 12, 24, 48 and 72 h on R2A agar at 45 u C. Gram staining was carried out by using the standard Gram reaction and was confirm...

Deep phylotaxonogenomic revision of the genus Xanthomonas within family Xanthomonadaceae and proposal of novel family Frateuriaceae within order Xanthomonadales

“…Here, according to the genus cut-off, all these eight genera are synonyms of genus Xanthomonas (Figure 1, Figure 2). These results also correlate with the whole genome-based phylogeny, as both this genus form a distinct phylogroup within the family (representative by the bold font in the phylogenomic tree) (Figure 1 2013 [34] and exclusion of Mizugakiibacter Kojima et al 2014 [35], Metallibacterium Ziegler et al 2013 [36] and Pseudolysobacter Wei et al 2020 [37] . The member genera of the family have been established by the latest deep phylotaxonogenomic analysis in the present study.…”

“…n. Rehaibacterium , a small rod from Rehai National Park. Type species of the genus is Rehaibacterium terrae [41]. The genus Rehaibacterium belongs to order Xanthomonadales and family Xanthomonadaceae , based on the deep phylotaxonogenomic analysis in the present study.…”

“…The proposed genus including Frateuriaceae includes the genus Frateuria Swings et al 1980 [38], Dokdonella Yoon et al 2006 [39], Pseudolysobacter Wei et al 2020 [37], Rudaea Weon et al 2009 [30], Tahibacter Makk et al 2014 [40]. Exclusion in Aquimonas Saha et al 2005 [32], Chiayiivirga Hsu et al 2013 [31], Rehaibacterium Yu et al 2013 [41] from previously described family Rhodanobacteraceae and inclusion of Mizugakiibacter Kojima et al 2014 [35], Metallibacterium Ziegler et al 2013 [36] and Pseudolysobacter Wei et al 2020 [37]. The member genera of the family have been identified by the latest deep phylotaxonogenomic analysis in the present study.…”

Membrane Bioreactor Pretreatment of High-Salinity O&G Produced Water

“…Both Roseovarius and Iodidimonas have been reported/isolated from PWs/hydrocarbon-contaminated environments from around the world; however, experimental evidence for hydrocarbon degradation by Roseovarius and Iodidimonas isolates is not available. − Isolates belonging to these genera have salinity tolerance in the range of 5–100 g/L. , Roseovarius and Iodidimonas have been identified as iodide oxidizing bacteria (IOB) because they can produce iodine from iodide in the presence of oxygen. , A wide range of salt tolerance, coupled with the reported bactericidal properties of iodine, may allow these genera to colonize the reactor first by inhibiting susceptible bacteria , Members of genus Methylophaga are primarily known for the metabolism of C1 compounds; however, one isolate has been reported to degrade alkanes ( n -hexadecane). , …”

“…9 Rehaibacterium is a newly described genus with one reported isolate so far, but a recent study correlated this genus with DOC removal in a BAF treating PW from Sichuan Basin, China. 55,56 Members of genus Methylophaga are primarily known for metabolism of C1 compounds; however, one isolate has been reported to degrade alkanes (n-hexadecane). 57, 58 As the salinity of the feed was further increased beyond TDS of 80 g/L, there was an increase in diversity, which coincided with an increase in abundance of other genera, including Marinobacter, Malaciobacter, and Marinobacterium.…”