Relation between Blood Parameters and Platelet-Rich Fibrin Membrane Size for Ocular Graft

Abstract

Purpose: To investigate the relationship between blood parameters and platelet-rich fibrin (PRF) membrane size for ocular graft. Methods: Total of 12 New Zealand white rabbits, 6.5 ml of blood samples was taken for each rabbit, including 5 ml for platelet-rich fibrin membrane production using L-PRF protocol (centrifugation at 2700 rpm for 12 minutes) 1.5 ml for evaluation of blood parameters. Measurement of blood parameters consists of fibrinogen, haemoglobin, hematocrit, red blood cell (RBC), white blood cell (WBC) and platelet. Measurement of the platelet-rich fibrin membrane area was done using a calibrated photograph and ImageJ software. The Pearson correlation coefficient (r) was used to evaluate each scattergram statistically. Results: Linear regression analysis found a significant positive effect on the PRF membrane area on the fibrinogen (r = 0.802, p = 0.02), WBC (r = 0.625, p = 0.03) and platelet (r = 0.839, p = 0.001). However, there was no apparent correlation to haemoglobin (r = 0.514, p = 0.087), RBC (r = 0.548, p = 0.065) and hematocrit (r = 0.454, p = 0.138). Conclusions: The area of the PRF membrane has a positively significant relation to the fibrinogen, WBC and platelets. The data in this study can be used to estimate the size of the PRF membrane as an ocular graft.

Understanding Solid-Based Platelet-Rich Fibrin Matrices in Oral and Maxillofacial Surgery: An Integrative Review of the Critical Protocol Factors and Their Influence on the Final Product

“…An animal model study observed, using a linear regression analysis, a significant positive effect on the PRF membrane area on the fibrinogen (Pearson correlation coefficient [r] = 0.802; p = 0.02), WBCs (r = 0.625; p = 0.03), and platelets (r = 0.839; p = 0.001). However, there was no apparent correlation between haemoglobin (r = 0.514; p = 0.087), RBCs (r = 0.548; p = 0.065), and haematocrit (r = 0.454; p = 0.138) [60]. Miron et al [61] observed in vivo a larger PRF membrane size in women and patients older than 65 years due to higher haematocrit counts in these patients.…”

Platelet-rich fibrin (PRF) graft and amniotic membrane graft on transforming growth factor-β (TGF-β) and type 1 collagen post conjunctival excision

“…1,7 Platelet-rich fibrin membrane (PRF) is a second-generation platelet concentrate developed by Choukroun et al from France, which is widely used in oral and maxillofacial, plastic surgery, orthopedic and ophthalmic surgery. 8 The PRF membrane has a mechanical function as a framework for cell proliferation, differentiation, and migration that is important for tissue regeneration. The PRF membrane also contains many growth factors that are important in the wound healing process, such as transforming…”