Relation between primary and secondary metabolism in plant cell suspensions

Plas, L.H.W. van der; Eijkelboom, C.; Hagendoorn, M.J.M.

doi:10.1007/bf00052164

Cited by 44 publications

(5 citation statements)

References 13 publications

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“…Overall, the effect of elicitors on cell growth is dependent on elicitors, used concentrations or conditions, plant species, the stage when the elicitor is applied, etc. Elevated secondary metabolism is often negatively correlated with cell growth (van der Plas et al 1995), but in this study, the production of anthocyanin and phenolic acid was stimulated by chitosan, pectin Fig. 4 Antioxidant activity (a), medium pH value (b) and conductivity (c) of V. vinifera cell culture.…”

Section: Discussionmentioning

confidence: 66%

Polysaccharide elicitors enhance anthocyanin and phenolic acid accumulation in cell suspension cultures of Vitis vinifera

Cai¹,

Kastell²,

Mewis

et al. 2011

Plant Cell Tiss Organ Cult

116

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The effects of yeast extract and selected polysaccharide elicitors on secondary metabolite production, particularly of anthocyanin and phenolic acid, in cell suspension cultures of Vitis vinifera were investigated. All elicitors either maintained or promoted cell growth in culture. Overall, secondary metabolite production in V. vinifera cell suspension cultures responded differently to different elicitors. Chitosan, pectin, and alginate enhanced production of anthocyanin within 13 days of culture with levels of 2.5-, 2.5-, and 2.6-fold increase, respectively, over that of control. Chitosan, alginate, and gum arabic significantly promoted accumulation of phenolic acids, particularly 3-O-glucosyl-resveratrol, in V. vinifera cultures, as well as in the culture medium. Intracellular phenolic acid production was significantly enhanced by alginate and chitosan, with 1.7-and 1.5-fold levels, respectively, of that of control. Extracellular phenolic acid production was also significantly increased in the presence of chitosan and gum arabic, with levels of 3.3-and 1.7-fold higher, respectively, than those of control. In addition, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity was enhanced in the presence of elicitors, and this was positively correlated with increased accumulation of anthocyanin in V. vinifera cell suspension cultures.

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Section: Discussionmentioning

confidence: 66%

Polysaccharide elicitors enhance anthocyanin and phenolic acid accumulation in cell suspension cultures of Vitis vinifera

Cai¹,

Kastell²,

Mewis

et al. 2011

Plant Cell Tiss Organ Cult

116

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“…In our findings, clone H5 line has a rapid accumulation of biomass and secondary metabolite; however, the PGR treatment did not enhance biomass production and tended to reduce fresh weights. Plant growth and defenses are restricted by their internal resources, and secondary metabolism often is negatively correlated with cell growth (Van Der Plas et al 1995 ). In addition, hairy root cultures must strike a balance between growth processes and the production of defensive compounds.…”

Section: Resultsmentioning

confidence: 99%

Establishment of hairy root lines and analysis of iridoids and secoiridoids in the medicinal plant Gentiana scabra

et al. 2014

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BackgroundGentiana scabra is commonly known as ‘Longdan’ is an important herb in traditional Chinese medicines, commonly used for the treatment of inflammation, anorexia, indigestion and gastric infections. Iridoids and secoiridoids are main bioactive compounds which attributed to the pharmacological properties of this plant. The use of hairy root cultures as an excellent alternative for the production of pharmaceutically important metabolites in less time period with ensured quality of raw materials.ResultsAn efficient hairy root culture system of Gentiana scabra and influence of different plant growth regulators (PGRs) on the production of gentiopicroside, swertiamarin and loganic acid constituents were described. Leaf explants were infected with Agrobacterium rhizogenes, which induced hairy roots up to 21%. The transformed hairy root lines were confirmed by PCR using rolB and rolC gene-specific primers. Among various solid and liquid media, B5 liquid medium resulted maximum root biomass (36- fold higher) in 4-weeks. Quantitative analysis showed loganic acid was 6.6- fold higher in the presence of zeatin (1 mg/l) and gentiopicroside accumulation was 1.8- fold higher in the presence of naphthaleneacetic acid (NAA, 1 mg/l), as compared to the roots of plants grown in greenhouse. On the other hand, 1.4- and 2.5- fold higher gentiopicroside and swertiamarin were observed in the presence of 1.0 mg/l NAA as compared to commercial Gentiana herb No. 2. The result also showed iridoid and secoiridoid contents affected greatly by age, physiology and growing environment of the plant.ConclusionsThe use of hairy root cultures is an excellent alternative to harvesting natural or in vitro grown plants to produce pharmaceutically important metabolites in less time with ensured quality.Electronic supplementary materialThe online version of this article (doi:10.1186/1999-3110-55-17) contains supplementary material, which is available to authorized users.

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“…Indeed, it must be stressed that the enhanced production comes from the enhanced activity of the enzymes involved in the phenolic pathway, including phenylalanine ammonia lyase and chalcone synthase. In addition, the enzyme activity of PEP (phosphoenolpyruvate)-carboxylase is enhanced, and this suggests a reallocation from sucrose production to defensive metabolite production [ 15 , 98 , 101 , 141 , 142 , 143 , 144 , 145 , 146 , 147 , 148 ].…”

Section: Costs Of Resistancementioning

confidence: 99%

Carbon Fluxes between Primary Metabolism and Phenolic Pathway in Plant Tissues under Stress

Caretto

Linsalata

Colella

et al. 2015

IJMS

251

151

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Higher plants synthesize an amazing diversity of phenolic secondary metabolites. Phenolics are defined secondary metabolites or natural products because, originally, they were considered not essential for plant growth and development. Plant phenolics, like other natural compounds, provide the plant with specific adaptations to changing environmental conditions and, therefore, they are essential for plant defense mechanisms. Plant defensive traits are costly for plants due to the energy drain from growth toward defensive metabolite production. Being limited with environmental resources, plants have to decide how allocate these resources to various competing functions. This decision brings about trade-offs, i.e., promoting some functions by neglecting others as an inverse relationship. Many studies have been carried out in order to link an evaluation of plant performance (in terms of growth rate) with levels of defense-related metabolites. Available results suggest that environmental stresses and stress-induced phenolics could be linked by a transduction pathway that involves: (i) the proline redox cycle; (ii) the stimulated oxidative pentose phosphate pathway; and, in turn, (iii) the reduced growth of plant tissues.

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Relation between primary and secondary metabolism in plant cell suspensions

Cited by 44 publications

References 13 publications

Polysaccharide elicitors enhance anthocyanin and phenolic acid accumulation in cell suspension cultures of Vitis vinifera

Polysaccharide elicitors enhance anthocyanin and phenolic acid accumulation in cell suspension cultures of Vitis vinifera

Establishment of hairy root lines and analysis of iridoids and secoiridoids in the medicinal plant Gentiana scabra

Carbon Fluxes between Primary Metabolism and Phenolic Pathway in Plant Tissues under Stress

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