Relationship between Active Oxygen Species, Lipid Peroxidation, Necrosis, and Phytoalexin Production Induced by Elicitins in Nicotiana

Rustérucci, Christine; Stallaert, Veerle; Milat, Marie‐Louise; Pugin, Alain; Ricci, Pierre; Blein, Jean‐Pierre

doi:10.1104/pp.111.3.885

Cited by 133 publications

(91 citation statements)

References 37 publications

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“…The necrotic activity was assessed on fully expanded leaves weighing 3-5 g. Leaves were excised and treated with elicitins (Rustérucci et al, 1996). A 10-l drop of the selected elicitin aqueous solution (0.1-1 nmol) was put on the petiole of leaves and followed three times with 10 l of water to ensure full absorption of the elicitor.…”

Section: Biological Assaysmentioning

confidence: 99%

“…The natural elicitins that harbor strong biological activities Rustérucci et al, 1996) also display high efficiency for loading sterols (Mikes et al, 1998). However, although elicitins exhibit differential biological activities, interactions with their putative receptors were reported to be similar (Bourque et al, 1998).…”

Section: Are High-affinity-binding Sites the Elicitin Receptors?mentioning

confidence: 99%

“…The most efficient elicitin is cryptogein (from Phytophthora cryptogea), the less efficient being parasiticein and capsicein, secreted by Phytophthora parasitica and Phytophthora capsici, respectively. Moreover, cryptogein induces stronger cell responses than do parasiticein and capsicein (Rustérucci et al, 1996;Bourque et al, 1998). This suggests an apparent correlation between the elicitor activities of natural elicitins and their ability to load and transfer sterols.…”

Section: Introductionmentioning

confidence: 97%

“…In tobacco plants, these elicitors induce both a hypersensitive response (leaf necrosis) and nonspecific systemic acquired resistance (SAR; Bonnet et al, 1996;Keller et al, 1996). In cell suspension cultures, classical mechanisms of elicitation have been reported, such as calcium influx (Tavernier et al, 1995), alkalization of the extracellular medium (Blein et al, 1991), production of active oxygen species (Rustérucci et al, 1996;Simon-Plas et al, 1997), and cell wall modifications (Kieffer et al, 2000). The presence of a single family of high-affinity sites for elicitins has been reported (Blein et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

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Mediation of Elicitin Activity on Tobacco Is Assumed by Elicitin-Sterol Complexes

Osman¹,

Vauthrin²,

Mikeš

et al. 2001

MBoC

105

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Elicitins secreted by phytopathogenic Phytophthora spp. are proteinaceous elicitors of plant defense mechanisms and were demonstrated to load, carry, and transfer sterols between membranes. The link between elicitor and sterol-loading properties was assessed with the use of site-directed mutagenesis of the 47 and 87 cryptogein tyrosine residues, postulated to be involved in sterol binding. Mutated cryptogeins were tested for their ability to load sterols, bind to plasma membrane putative receptors, and trigger biological responses. For each mutated elicitin, the chemical characterization of the corresponding complexes with stigmasterol (1:1 stoichiometry) demonstrated their full functionality. However, these proteins were strongly altered in their sterol-loading efficiency, specific binding to high-affinity sites, and activities on tobacco cells. Ligand replacement experiments strongly suggest that the formation of a sterol-elicitin complex is a requisite step before elicitins fasten to specific binding sites. This was confirmed with the use of two sterol-preloaded elicitins. Both more rapidly displaced labeled cryptogein from its specific binding sites than the unloaded proteins. Moreover, the binding kinetics of elicitins are related to their biological effects, which constitutes the first evidence that binding sites could be the biological receptors. The first event involved in elicitin-mediated cell responses is proposed to be the protein loading with a sterol molecule.

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Section: Biological Assaysmentioning

confidence: 99%

Section: Are High-affinity-binding Sites the Elicitin Receptors?mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mediation of Elicitin Activity on Tobacco Is Assumed by Elicitin-Sterol Complexes

Osman¹,

Vauthrin²,

Mikeš

et al. 2001

MBoC

105

View full text Add to dashboard Cite

show abstract

“…At the same time, a strong and rapid alkalization of the extracellular medium and a concomitant acidification of the cytosol are observed [20,22]. Few minutes later, a transient oxidative burst is noticed [22,23]. Cryptogein elicits an extracellular production of O 2 -on tobacco cells which is then dismutated in H 2 O 2 by extracellular superoxide dismutases.…”

mentioning

confidence: 99%

Involvement of lipid-protein complexes in plant-microorganism interactions

Blein¹

2002

OCL

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Summary : Increasing concerns about the environmental impact of modern agricultural have prompted research for alternate practices to pesticide treatments, notably using plant defense mechanisms. Thus, isolation and characterization of plant defense elicitors have been the main step of studies in many groups. Moreover, in the global concept of interactions between organisms and their environment, a major concern is to discriminate recognition between exogenous and endogenous signals, notably during pathogenic or allergenic interactions involving small proteins, such as elicitins or lipid transfer proteins (LTPs). Elicitins and lipid transfer proteins (LTP) are both able to load and transfer lipidic molecules and share some structural and functional properties. While elicitins are known as elicitors of plant defense mechanisms, the biological function of LTPs is still an enigma. They are ubiquitous plant proteins able to load and transfer hydrophobic molecules such as fatty acids or phospholipids. Among them, LTPs1 (type 1 lipid transfer proteins) constitute a multigenic family of secreted plant lipid binding proteins that are constitutively expressed in specific tissues and/or induced in response to biotic and abiotic stress (for reviews [1][2][3][4]). Their biological function is still unknown, even if some data provide arguments for a role of these proteins in the assembly of extracellular hydrophobic polymers (i.e., cutin and suberin) [2,4] and/or in plant defense against fungal pathogens [1,3]. Beside their involvement in plant defense, LTPs1 are also known to be pan-allergens of plant-derived foods [5]. Finally, the discovery of the sterol carrier-properties of elicitins has opened new perspectives dealing with the relationship between this function and the elicitor activity of these small cystein-rich proteins. Nevertheless, this elicitor activity is restrained to few plant species, and thus does not appear in accordance with a universal lipid transfer function. These considerations required a reassessment of the precise role of elicitins for both fungi and plants [6].Résumé : La stimulation des mécanismes naturels de défense des plantes représente une stratégie alternative à l'utilisation de la lutte chimique en phytoprotection. Dans ce contexte, les élicitines, qui sont de petites protéines sécrétées par des Phytophthora et des Pythium, induisent une réaction de type hypersensible chez des plantes comme le tabac. Ces plantes deviennent résistantes à l'agression de leurs agents pathogènes. La récente découverte que les élicitines sont des protéines de transfert de stérols a apporté une vue nouvelle sur l'activité moléculaire des élicitines. Élicitines et protéines de transfert de lipides (LTP) sont capables de charger et de transporter des molécules lipidiques et partagent des caractères communs tant au plan structural que fonctionnel mais, tandis que les élicitines sont connues pour leur activité élicitrice de réaction de défense chez les plantes, l'activité biologique des LTP reste inconnue. Cependant, ...

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Copper toxicity and date palm (Phoenix dactylifera) seedling tolerance: Monitoring of related biomarkers

Chaâbene

Hakim

Rorat

et al. 2017

Enviro Toxic and Chemistry

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Date palm (Phoenix dactylifera) seeds were exposed to different copper (Cu) solutions to examine plant stress responses. Low Cu concentrations (0.02 and 0.2 mM) caused an increase of seed germination, whereas higher Cu amounts (2 mM) significantly inhibited seed germination, delayed hypocotyl elongation, increased seedling mortality, and reduced the germination index by more than 90%. Metal-related toxicity symptoms appeared after 15 d of 2 mM of Cu exposure. Biochemical activities such as amylase activity and redox balance elements were examined to study the relationship between external Cu amount and internal plant response. The present study showed that amylolytic activity was dose- and time-dependent. Likewise, H O production increased after exposure to Cu, which was correlated with thiobarbituric acid reactive substance (TBARS) accumulation. Furthermore at low Cu concentrations, superoxide dismutase (SOD) and catalase (CAT) activities increased, suggesting that date palm seed stimulated its metal homeostasis networks. However, the highest cupric ion amounts increased cell oxidant accumulation and reduced enzyme production. Gene expression level measures of P. dactylifera phytochelatin synthase (Pdpcs) and P. dactylifera metallothionein (Pdmt) encoding genes have been carried out to investigate the implication of PdPCS and PdMT proteins in Cu homeostasis and/or its sequestration. Phoenix dactylifera metallothionein induction reached a peak after 30 d of exposure to 0.2 mM of Cu. However, it was down-regulated in plants exposed to higher Cu concentrations. In the same conditions, Pdpcs was overexpressed during 1 mo of exposure before it decreased thereafter. These observations provide a new insight into date palm cell response to Cu, a metal that can be toxic but that is also an essential element. Environ Toxicol Chem 2018;37:797-806. © 2017 SETAC.

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Relationship between Active Oxygen Species, Lipid Peroxidation, Necrosis, and Phytoalexin Production Induced by Elicitins in Nicotiana

Cited by 133 publications

References 37 publications

Mediation of Elicitin Activity on Tobacco Is Assumed by Elicitin-Sterol Complexes

Mediation of Elicitin Activity on Tobacco Is Assumed by Elicitin-Sterol Complexes

Involvement of lipid-protein complexes in plant-microorganism interactions

Copper toxicity and date palm (Phoenix dactylifera) seedling tolerance: Monitoring of related biomarkers

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