Relationship between Antitumor Efficiency of Photodynamic Therapy with Photoditasine and Photoenergy Density

Romanko, Yu. S.; Цыб, А Ф; Kaplan, M. A.; Попучиев, В. В.

doi:10.1007/s10517-005-0322-2

Cited by 23 publications

(19 citation statements)

References 7 publications

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“…Since cell damage is obviously so profound that repair mechanisms are not operative, death pathways are eventually activated. As reported in part previously [52][53][54], transcripts of cyclin family genes CCNC, CCND1 and CCNG1 as well as of PCNA, a protein crucial for DNA replication, were markedly reduced after PDT in our model thereby possibly leading to a diminished proliferative activity. However, the observed downregulation of cyclininteracting transcription factor E2F1 may also directly promote cell death, as reported previously for PC-3 cells [55].…”

Section: Discussionsupporting

confidence: 78%

Cellular and molecular effects of the liposomal mTHPC derivative Foslipos in prostate carcinoma cells in vitro

Gyenge

Hiestand

Graefe

et al. 2011

Photodiagnosis and Photodynamic Therapy

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BACKGROUND: Meso-tetra-hydroxyphenyl-chlorine (mTHPC) is among the most powerful photosensitizers available for photodynamic therapy (PDT). However, the mechanisms leading to cell death are poorly understood. We here focused on changes at DNA and RNA levels after treatment with the liposomal mTHPC derivative Foslipos in vitro. METHODS: After determination of darktoxicity, laser conditions and uptake kinetics, PC-3 prostate carcinoma cells were subjected to PDT with Foslipos, followed by assessment of cell numbers directly (TP0) or 1h (TP1), 2h (TP2), 5h (TP5) and 24h (TP24) after illumination. Nucleic acids had been extracted for evaluation of RNA amounts and integrity as well as for estimation of abasic sites as a measure for DNA damage. Furthermore, expression changes of 84 genes related to oxidative stress were investigated by quantitative polymerase chain reaction. RESULTS: Already at TP0, the number of dead cells was significantly higher after PDT versus controls and at TP24 more than 90% of cells had been destroyed. PDT resulted in a severe damage of both RNA and DNA. Gene expression analyses revealed an impact of PDT on pathways for oxidative and metabolic stress, heat shock, proliferation and carcinogenesis, growth arrest, inflammation, DNA repair and apoptosis signaling. CONCLUSIONS: Mechanisms of Foslipos-mediated PDT comprise a combination of acute and delayed lethal effects in PC-3 cells. The latter may include death processes initiated by nucleic acid damage, activation of stress and growth arrest genes in combination with a reduced capability to adequately cope with oxidative toxicity. Our results will help to better understand molecular photodynamic effects. AbstractBackground: Meso-tetra-hydroxyphenyl-chlorine (mTHPC) is among the most powerful

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Section: Discussionsupporting

confidence: 78%

Cellular and molecular effects of the liposomal mTHPC derivative Foslipos in prostate carcinoma cells in vitro

Gyenge

Hiestand

Graefe

et al. 2011

Photodiagnosis and Photodynamic Therapy

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“…As shown in Figure d, compared to control groups, there were many fewer PCNA positive cells in the AuNC@DHLA TP-PDT group, indicating lower levels of tumor cell proliferation. Our results were consistent with previous research which showed that PDT impaired tumor cell proliferation . Taken together, the above results demonstrate that AuNC@DHLA TP-PDT is highly effective in inhibiting the growth of xenograft tumors.…”

Section: Resultssupporting

confidence: 93%

Super-efficient in Vivo Two-Photon Photodynamic Therapy with a Gold Nanocluster as a Type I Photosensitizer

et al. 2019

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Photodynamic therapy (PDT) is a clinically approved, minimally invasive therapeutic technique that can induce the regression of targeted lesions via generating excess cytotoxic reactive oxygen species. However, due to the limited penetration depth of visible excitation light and the intrinsic hypoxia microenvironment of solid tumors, the efficacy of PDT in the treatment of cancer, especially deep-seated or large tumors, is unsatisfactory. Herein, we developed an efficient in vivo PDT system based on a nanomaterial, dihydrolipoic acid coated gold nanocluster (AuNC@DHLA), that combined the advantages of large penetration depth in tissue, extremely high two-photon (TP) absorption cross section (σ 2 ∼ 10 6 GM), efficient ROS generation, a type I photochemical mechanism, and negligible in vivo toxicity. With AuNC@DHLA as the photosensitizer, highly efficient in vivo TP-PDT has been achieved.

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“…It was interesting to observe that in tumors treated with methylene blue nanoparticles, the percentage of PCNA + cells was less than that in other control groups, indicating lower levels of cell proliferation. Previous research has shown impaired tumor cell proliferation after PDT [35]. …”

Section: Discussionmentioning

confidence: 99%

Nanoparticle-mediated combination chemotherapy and photodynamic therapy overcomes tumor drug resistance

Khdair

Chen

Patil

et al. 2010

Journal of Controlled Release

253

158

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Tumor drug resistance significantly limits the success of chemotherapy in the clinic. Tumor cells utilize multiple mechanisms to prevent the accumulation of anticancer drugs at their intracellular site of action. In this study, we investigated the anticancer efficacy of doxorubicin in combination with photodynamic therapy using methylene blue in a drug-resistant mouse tumor model. Surfactant-polymer hybrid nanoparticles formulated using an anionic surfactant, Aerosol-OT™ (AOT), and a naturally occurring polysaccharide polymer, sodium alginate, were used for synchronized delivery of the two drugs. Balb/c mice bearing syngeneic JC tumors (mammary adenocarcinoma) were used as a drug-resistant tumor model. Nanoparticle-mediated combination therapy significantly inhibited tumor growth and improved animal survival. Nanoparticle-mediated combination treatment resulted in enhanced tumor accumulation of both doxorubicin and methylene blue, significant inhibition of tumor cell proliferation, and increased induction of apoptosis. These data suggest that nanoparticle-mediated combination chemotherapy and photodynamic therapy using doxorubicin and methylene blue has significant therapeutic potential against drug-resistant tumors.

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Relationship between Antitumor Efficiency of Photodynamic Therapy with Photoditasine and Photoenergy Density

Cited by 23 publications

References 7 publications

Cellular and molecular effects of the liposomal mTHPC derivative Foslipos in prostate carcinoma cells in vitro

Cellular and molecular effects of the liposomal mTHPC derivative Foslipos in prostate carcinoma cells in vitro

Super-efficient in Vivo Two-Photon Photodynamic Therapy with a Gold Nanocluster as a Type I Photosensitizer

Nanoparticle-mediated combination chemotherapy and photodynamic therapy overcomes tumor drug resistance

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