Relationship between biochemical, environmental, and microbiological changes with the success rate of fertilization techniques

Abstract

The success rate for the evaluation of the results of techniques of Assisted Human Reproduction implies the occurrence of pregnancy with a live, healthy child. There are reports of the association between chemical and unhealthy environmental air conditions (bacteria, dust, particulates and volatile compounds) and reducing the success rate of embryo production and the occurrence of pregnancy. This paper reviews available information about the interference of biochemical, environmental and microbiological changes with the success of the assisted reproductive technique.

Does the Fertilization and Reproduction Success in Iso 5/7 Labor Have a Difference When Compared to a Conventional Laboratory?

“…Knowing about the possible risk of contamination either from the gametes or from the internal air, shows that it is necessary to track the microbiological contamination in the human reproduction laboratory in order to investigate the association between microbiological contamination and success in human reproduction. 4 At the old LabRep-HC-UFG facilities, Foizer developed a post embryonic incubation microbiological analysis in the embryo culture mediums and found 6 contaminated plates in 125 samples (4,8%). The microorganisms found were Escherichia coli (50%), Klebsiella sp (16,6%), Pseudomonas sp (16,6%), Levedura (16,6%).…”

“…3 There are reports of an association between unsanitary chemical and environmental air conditions (e.g., bacteria, dust, particulate matter and volatile compounds) and reducing the rate of success in producing embryos and the occurrence of pregnancy. [4][5][6] AHP uses a set of low and high complexity techniques. Intrauterine insemination (IUI) is considered a low complexity technique, while extracorporeal fertilization methods are considered high complexity techniques.…”

“…The ISO 5/7 lab has a climatization system that keeps positive pressure in adjacent environments; temperature control conditions between 23°C to 27°C; relative air humidity between 40% and 70%; total air minimum flow on 45 (m 3 /h)/m 2 ); exterior air minimum flow on 15 (m 3 /h)m 2 and minimum filtering in filters insufflated with G3 (coarse filter) and F8 filters (fine filters capable of trapping particles of 0.4 µm). 4,14,15 Regarding the living embryos manipulation room, classified as ISO Class 5, it has a biological security cabinet Class 2, Type A and an unidirectional flow module. 9,11,16 This paper aims to verify if the success of fertilization and reproduction in the iso 5/7 laboratory are different when compared to a conventional laboratory?…”