Relationship between cell ploidy and glucocorticoid induced death in human lymphoid cell lines

Dyson, J. E.; Quirke, Philip; Bird, C. C.; McLaughlin, John B.; Surrey, C. R.

doi:10.1038/bjc.1984.115

Cited by 11 publications

(14 citation statements)

References 10 publications

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“…It was found that 36 tumours (72%) would have been correctly assigned on the basis of any one sample whereas 14 tumours (28%) could have been incorrectly designated since one of the samples fell into a different ploidy group from the others. If the tumours were further subdivided into three subgroups (<10%, 10-30%, >30% of Cell death Previous studies have confirmed the validity of using ethidium bromide staining to determine the level fo cell death in vivo and in vitro (Dyson et al, 1984a(Dyson et al, , 1984b(Dyson et al, , 1984c. The range of values found in the various tumours (8-92%) was the greatest of any of the areas quantitated ( Figure 4).…”

Section: \mentioning

confidence: 70%

“…Cells were again collected by centrifugation and resuspended in 2-4 ml of PBS (50%) and HBSS (50%); an equal volume of the same medium was then added containing 15,uM acridine orange (AO) and the sample was placed in the flow cytometer and the sample flow started and allowed to equilibriate. Figure 2 and were modified from our previous studies (Dyson et al, 1984a(Dyson et al, , 1984b(Dyson et al, , 1984c to allow for the wider range of DNA content seen in these tumours. Table IV.…”

Section: \mentioning

confidence: 99%

“…For these reasons we have employed in this study a combination of a newly developed flow cytometric technique (Dyson et al, 1984a;1984b;1984c) with conventional histopathological assessments of primary and metastatic colorectal adenocarcinomas. We have attempted to determine the extent of tumour heterogeneity and the proportion of dying cells to assess their influence on tumour staging and grading.…”

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confidence: 99%

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Heterogeneity of colorectal adenocarcinomas evaluated by flow cytometry and histopathology

Quirke¹,

Dyson²,

Dixon³

et al. 1985

Br J Cancer

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108

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Section: \mentioning

confidence: 70%

Section: \mentioning

confidence: 99%

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confidence: 99%

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Heterogeneity of colorectal adenocarcinomas evaluated by flow cytometry and histopathology

Quirke¹,

Dyson²,

Dixon³

et al. 1985

Br J Cancer

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108

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“…Calculation of percent of cells in phases of the cell cycle was carried out from the DNA content profile by means of a cell cycle analysis programme developed by Watson et al (1987). Cell viability This was assessed by differential permeability to the stains ethidium bromide and acridine orange, determined by flow cytometric measurement (Dyson et al, 1984b).…”

Section: Cell Culturementioning

confidence: 99%

The effect of sodium butyrate on the growth characteristics of human cervix tumour cells

Dyson¹,

Daniel²,

Surrey³

1992

Br J Cancer

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Summary Sodium butyrate has been shown to affect cell proliferation, and, at concentrations above approximately 0.5 mM, to cause cell death in some tumour cell lines. When combined with cytotoxic drugs increase in chemosensitivity has been observed. We are presently carrying out a study of the combined effects of sodium butyrate and cytotoxic drugs on cultured cervix tumour cells. To provide a baseline for this study we Prasad, 1980;Kruh, 1982;Nordenberg et al., 1987). Possibly combined with the effect of n-butyrate on cell proliferation is its action in reversibly inducing what has been termed a 'better differentiated phenotype', of increased radiosensitivity and chemosensitivity (Spremuli & Dexter, 1984). Due to these properties n-butyrate has been the subject of clinical investigations in leukaemic patients (Novogrodsky et al., 1983;Miller et al., 1987). Its effects when combined with cytotoxic drugs (Wasserman et al., 1989) or irradiation (Arundel & Leith, 1987; Leith et al., 1986) have also been investigated.We are presently carrying out an investigation of the effect of n-butyrate, when combined with a range of cytotoxic drugs, on cervix tumour cell lines when cultured as multicell spheroids. As a necessary preliminary to this study we have carried out a systematic investigation of the effect of nbutyrate alone on the growth characteristics of cervix tumour cells cultured as multicell spheroids. The effect of n-butyrate concentrations in the range 0.005 to 3 mM has been investigated, and the culture of the cells as multicell spheroids has allowed measurements over periods of up to 24 days. The results of this study are presented in this report. Materials and methods Cell cultureThe cervix tumour cell lines employed in this study were established in primary culture from cervix biopsy tissue taken routinely during radiotherapy at Cookridge Hospital (Dyson et al., 1984a). Six cell lines were used: 754, 612, 995, 090, 329, 708. These were maintained as monolayer cultures from which multicellular aggregates were obtained as required by using the method of Sutherland and Durand (1976). Spheroid cultures were initiated with the same number of spheroids per flask to allow intercomparison of cell counts during the period of the experiments. Multicell spheroids were maintained in culture as previously described (Boothby et al., 1989). Excess spheroids were discarded at the time of media change to maintain cell numbers approximately constant.The necessary volumes of 50 mM or 500 mM sodium nbutyrate solution were added to the spheroid suspensions at the start of the experiment to adjust to the concentrations shown in the figures, with further additions at media changes to maintain these concentrations. The butyrate solution was prepared from n-butyric acid (BDH Limited, Poole, UK) in Hanks basic salt solution, adjusted to pH 7.2 with NaOH solution, then sterilised by filtration through an 0.2 gtm Acrodisc (Gelman Sciences Limited, Northampton, UK).Spheroid diameter This was measured by means of a laser diffracti...

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“…Similarly, a unit of BCGF-II is the dilution of factor required to give halfmaximal proliferation of the BCL, lymphoma (25 bovine serum albumin at 4°C. They were stained with acridine orange and ethidium bromide to final concentrations of 15 ,uM and 3 ,uM, respectively (28,29), and analyzed on a Cytofluorograph system 50H (Ortho Diagnostic Systems, Westwood, MA). The excitation wavelength has 488 nm with collection of green fluorescence at 530-565 nm and red fluorescence at >640 nm (29,30).…”

Section: Methodsmentioning

confidence: 99%

B-cell-stimulatory factor 1 reverses Fc receptor-mediated inhibition of B-lymphocyte activation.

O’Garra¹,

Rigley²,

Holman³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

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Intact (IgG class) rabbit anti-immunoglobulin antibodies are not mitogenic for mouse B cells but inhibit proliferation induced by F(ab')2 anti-Fab fragment antibodies (anti-Ig). In addition, cross-linkage of Fc and surface immunoglobulin receptors on B cells by intact anti-Ig inhibits inositol phospholipid breakdown (but not Ca2+ flux) resulting from ligation of antigen receptors. This system, therefore, provides a polyclonal model for B-cell inactivation by antigen-antibody complexes. T-cell-derived B-cell-stimulatory factor 1 acts syn. ergistically with submitogenic concentrations of F(ab')2 anti-Ig to induce B-cell proliferation. We show here that B-cellstimulatory factor 1 and intact anti-Ig also induce B cells to synthesize DNA. However, B-cell-stimulatory factor 1 does not induce inositol phospholipid breakdown, does not mobilize Ca2+ in B cells, nor does it influence the magnitude of these responses provoked by intact anti-Ig.

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Relationship between cell ploidy and glucocorticoid induced death in human lymphoid cell lines

Cited by 11 publications

References 10 publications

Heterogeneity of colorectal adenocarcinomas evaluated by flow cytometry and histopathology

Heterogeneity of colorectal adenocarcinomas evaluated by flow cytometry and histopathology

The effect of sodium butyrate on the growth characteristics of human cervix tumour cells

B-cell-stimulatory factor 1 reverses Fc receptor-mediated inhibition of B-lymphocyte activation.

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