The Fremont IAC 543 mandarin variety is a hybrid from the crossing of the Clementina and Ponkan mandarins and it can be grown on different rootstocks in Brazil. The objective of this research was to investigate the influence of different rootstocks (Rangpur lemon, Swingle citrumelo, trifoliate and Flying Dragon trifoliate) on the chemical composition of the essential oils and volatiles of the Fremont IAC 543 mandarin. The essential oils from the fruit peels were obtained by hydrodistillation, and ranged from 0.36 to 0.47% (v/w) and the mandarin cultivated in Flying Dragon trifoliate rootstock showed the lowest essential oil yield. The chemical characterization of the essential oils was performed by gas chromatography coupled to mass spectrometry (GC-MS) and the semi-quantification of essential oil components was performed by gas chromatography coupled to a flame ionization detector (GC-FID). The major essential oils compounds were limonene, linalool, and myrcene, which were responsible for more than 95% of the essential oil components. Due to the higher productivity of the citrumelo Swingle rootstock (18 L ha-1), this was the most suitable rootstock for the Fremont IAC 543 mandarin essential oil production. Aiming the characterization of the Fremont IAC 543 mandarin juice volatiles compounds, a multivariate optimization of juice volatiles extraction by headspace solid phase microextraction (HS-SPME) was performed. It was found that the best volatile extraction conditions were the use of divinylbenzene/polydimethylsiloxane (DVB/PDMS) fiber, 43 ° C for extraction temperature and 31 min for extraction time. The main volatile compounds of the whole juices studied were limonene, myrcene, linalool, decanal and α-pinene. It is possible to see a considerable variation in the volatile composition of whole juices according to the rootstock used. Nuclear Magnetic Resonance spectras showed that the juices of the four samples analyzed had complex but similar profiles. Tangerine cultivated with Rangpur lemon rootstock showed higher values of malic acid and tyrosine. Tangerine growth in citrumelo Swingle showed higher amounts of citric acid and ethanol. The variety planted on the rootstock of trifoliate showed higher amount of alanine, glucose and sucrose. The sample cultivated on the Flying Dragon trifoliate rootstock showed less ethanol. Through the principal component analysis, it was possible to differentiate the samples using their essential oil components and volatile and non-volatile compounds from the integral juice of each variety planted in different rootstocks.