Relationship Between DNA Synthesis and Water Content During Ripening of<i>Vicia Faba</i>Seed

Brunori, A.

doi:10.1080/00087114.1967.10796271

Cited by 39 publications

(18 citation statements)

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“…The dehydration data conc!usive!y demonstrate the inhibitory effect of drying on DNA rep!ication. This was a!so demonstrated by Brunori (1967) during maturation drying of Vicia faba seeds. During seed dehydration DNA synthesis ceased at and below 75% moisture content, while Gi-phase nuclei could continue their cell cycle and were later arrested in the G|)/G]-phase.…”

Section: Discussionmentioning

confidence: 73%

“…ity render this system ideal for the study of tbe survival Many studies have been perfortned on DNA content mechanisms of tissues after severe dehydration, and cell cyc!e stages in seeds during dry storage using Recent studies on desiccation tolerance focused microdensitometry or flow cytometry (Brunori and mainly on membrane behaviour and protection by carbo-D' Amato 1967, Bino et al, 1993, In many species the nuclei of the quiescent zygotic embryos are completely arrested in the presynthesis Gd/Grphase (for a review, see Bewley and Black 1994), This indicates a stringent control of the cell cycle during maturation and dehydration of seeds of these species, Deltour (1985) suggested that the general arrest in Gu/G] might render seeds more tolerant to stress conditions. However, in seeds of other species, such as Viciafaba (Brunori 1967) and tomato (Lycopersicon esculentum) ( Liu et al 1994), also nuclei in the postsynthesis G2-phase were detected, mainly originating from root tip cells. Likewise, primed seeds contained considerable numbers of nuclei in G2-phase (Bino et al 1992, and although they are high!y viable, they are more sensitive to dry storage (Lanteri et al 1994).…”

mentioning

confidence: 99%

“…It has been proposed that control of the nuclear division during maturation might be regulated through seed water activity (Brunori 1967) or ABA (Liu et al 1994). Brunori (1967) demonstrated in maturing Vicia faba seeds that DNA synthesis ceased below 75% water content on a fresh weight basis, whereas mitotic activity was inhibited be!ow 65% moisture content. This notwithstanding, a certain percentage of G2 nuclei remained.…”

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confidence: 99%

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Effect of ABA and slow drying on DNA replication in carrot (Daucus carota) embryoids

Tetteroo¹,

Bino²,

Bergervoet³

et al. 1995

Physiol Plant

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1995. Effect of ABA and slow drying on DNA replication in carrot (Daucus carota) embryoids. -Physiol. Plant. 95: 154-158.Addition of abscisic acid (ABA) at the torpedo-shaped stage of development and slow dehydration are two parameters necessary to produce completely desiccation-tolerant carrot {Daucus carota L.) embryoids. The mode of action of these parameters is still largely unidentified. Employing flow cytometry we investigated their effect on DNA replication and cell cycle activity of the developing embryoids. DNA replication was determir\ed as percentage of 4C nuclei. Addition of ABA did not alter DNA replication and cell cycle during embryoid development in vitro, in spite of the putative quiescent state of the torpedo-shaped embryoids. In contrast, during slow drying the nuclei were preferentially arrested in the presynthesis Ga/Grphase and the amount of Gi nuclei decreased. Dry zygotic carrot embryos do not contain any Gi nuclei and are completely desiccation tolerant. The decline of Gi nuclei in dry somatic embryoids seems to coitKide with the increase in desiccation tolerance, which is incomplete compared to zygotic embryos. Our results suggest that in order to withstand anhydrobiosis, DNA replication may be controlled during the embryoid developmental program and slow dehydration, but not by the plant growth regulator ABA.

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Section: Discussionmentioning

confidence: 73%

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confidence: 99%

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confidence: 99%

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Effect of ABA and slow drying on DNA replication in carrot (Daucus carota) embryoids

Tetteroo¹,

Bino²,

Bergervoet³

et al. 1995

Physiol Plant

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“…Brunori (1967) suggested that in a dehydrated embryo, the available water supply might limit DNA synthesis. The procambium, root and shoot apices and leaf primordia stain deeply for these metabolites during seed germination.…”

Section: Discussionmentioning

confidence: 99%

Histones and nucleic acids during seed development and germination in Linum usitatissimum.

1988

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“…Soon after the onset of maturation drying in seeds, DNA replication stops, to be followed in sequence by the cessation of RNA and protein synthesis (Brunori, 1967). The molecular changes associated with the last phases of desiccation are almost unknown and, as water becomes increasingly limiting, presumably will include non-enzymatic changes to macromolecules.…”

Section: Methylation Of Dna Gene Expression and Desiccation Tolerancementioning

confidence: 99%

DNA and desiccation tolerance

Osborne

Boubriak

1994

Seed Sci. Res.

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This article reviews mechanisms by which specialized cells of different life forms have overcome the lethal effects of dehydration and considers how the maintenance of genetic information is central to survival As a dynamic and hydrated moleculein vivo, DNA can assume different conformational structures depending upon the water activity, the base sequence and the presence of specific binding proteins The attainment of stable secondary structures that are resistant to degradation in vivo at low water potentials is proposed as a likely accompaniment to desiccation tolerance In addition, chemical modification of bases in DNA, the extent of methylation and conformational changes could determine the expression of different gene sequences as cells pass from desiccation-tolerant to desiccationintolerant states We monitored the integrity of extracted DNA in embryos of seeds and in winddispersed pollen during transition from their desiccation tolerance to desiccation intolerance on hydration and germination. We present evidence to show that the DNA of these two stages is different and that it is the DNA from desiccation-tolerant cells only that retains integrity when the cells are subjected to desiccation regimes We discuss these findings in relation to certain hydration-sensitive DNA structures and to other relevant biological systems

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Relationship Between DNA Synthesis and Water Content During Ripening ofVicia FabaSeed

Cited by 39 publications

References 4 publications

Effect of ABA and slow drying on DNA replication in carrot (Daucus carota) embryoids

Effect of ABA and slow drying on DNA replication in carrot (Daucus carota) embryoids

Histones and nucleic acids during seed development and germination in Linum usitatissimum.

DNA and desiccation tolerance

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