Relationship between endometritis and oxidative stress in the follicular fluid and luteal function in the buffalo

Abstract

In this study, alteration in the follicular fluid composition and luteal function was investigated in the buffalo with endometritis. Genitalia were classified into cytological and purulent endometritis on the basis of polymorphonuclear cell cut off while non-endometritis served as control (n = 10/group). In the follicular phase, the number of surface follicles was counted, diameter of the largest follicle was measured and the follicular fluid was assayed for total protein, cholesterol, malondialdehyde (MDA), total antioxidant capacity (TAC), oestradiol (E2 ) and progesterone (P4 ). The P4 content of corpus luteum during mid-luteal phase was estimated by radioimmunoassay. Ovaries from the follicular phase of oestrous cycle showed no significant difference in the total number of surface follicles, size of the largest follicle and volume of follicular fluid in the buffaloes with and without endometritis (p > .05). However, the antral fluid of the largest follicle from the genitalia of buffalo with cytological and purulent endometritis showed a significant decrease in the concentration of total protein, cholesterol, TAC and E2 and a significant increase in the concentration of MDA and P4 (p < .05). The results indicated that there is an association between endometritis and decreased ovarian function.

Construction of Competing Endogenous RNA Networks Incorporating Transcription Factors to Reveal Differences in Granulosa Cells from Patients with Endometriosis

“…Therefore, the normal function of GCs is crucial to the growth, development, and maturation of oocytes (Yamamoto et al, 2015;Guo et al, 2016). Existing studies showed that the follicular development potentially decreased, the apoptotic rate of GCs increased, and the changes in follicular microenvironment and endocrine occurred in EM-related infertility (Gupta et al, 2008;Behera et al, 2016). Poor quality of oocytes might be closely related to GCs in follicles in patients with EM-related infertility.…”

Immunomodulatory effect of curcumin on lipopolysaccharide‐ and/or flagellin‐induced production of prostaglandin E2 and relative expression of proinflammatory cytokines in the primary bubaline endometrial stromal cells

“…Failure of the innate uterine defence mechanism to eliminate the bacteria by 3 weeks PP increases the risk of endometritis. In the buffalo, the incidence of PP endometritis is reported to be 20%, and subclinical endometritis (SE) is a major problem (Behera et al, ; Rao & Sreemannarayana, ). Escherichia coli (E. coli), main pathogen isolated from SE, carries pathogen‐associated molecular patterns (PAMP) like lipopolysaccharide (LPS) that are recognized by the pattern recognition receptors (PRR) like toll‐like receptors (TLR) of the host.…”