Relationship Between Essential Hypertension and Single Nucleotide Polymorphism of Apolipoprotein E Gene in Han, Miao, and Buyi Populations in Guizhou

Abstract

Background To investigate the correlation between single nucleotide polymorphism (SNP) of human Apolipoprotein E (ApoE) gene and essential hypertension (EH) in Han, Miao, and Buyi populations in Guizhou. Methods A case–control study was conducted. A total of 343 patients with EH and 335 healthy controls were selected from Leishan Miao, Libo Buyi, and Guiyang Han populations in Guizhou. The clinical data and biochemical indicators including age, gender, height, weight, fasting blood glucose, cholesterol, and triglyceride were collected. The t test or chi-square test was used to compare the differences of clinical data and biochemical indexes between the 2 groups. Sequenom MassARRAY was used to detect genotype of 5 SNP (rs7259620, rs440446, rs769449, rs1065853, and rs439401) and kompetitive allele-specific PCR (KASP) was used to detect 2 SNP (rs429358 and rs7412) of ApoE gene. Logistic regression was used to analyze the genetic relationship between ApoE gene polymorphisms and EH in Guizhou populations. Results Age, overweight, and obesity were independent risk factors for EH in Guizhou populations. Fasting blood glucose and triacylglycerol are independent risk factors for EH in Guizhou Han population. In the Miao and Buyi populations in Guizhou, the correlation between EH and the studied SNP was not statistically significant. In the Han population, the distribution of rs439401 allele and genotype between EH group and control group was significantly different (χ2 = 6.438, P = 0.011; χ2 = 6.389, P = 0.041). Compared with the TT or TC genotype, the genotype CC increased the risk of EH (OR = 2.164, 95% CI 1.035–4.526, P = 0.040). Compared with haplotype G-G-T (rs7259620–rs1065853–rs439401), haplotype G-G-C reduced the risk of EH in Han population (OR = 0.465, 95% CI 0.263–0.824, P = 0.008). Multifactor dimensionality reduction analysis results showed that rs440446, rs429358, and rs7412 had an interaction effect on the occurrence of EH in Miao population (χ2 = 10.012, P = 0.002); rs7259620, rs1065853, and rs439401 had an interaction effect on the occurrence of EH in Buyi population (χ2 = 3.972, P = 0.046); rs7259620, rs440446, and rs439401 had an interaction effect on the occurrence of EH in Han population (χ2 = 10.155, P = 0.001). Conclusions The rs439401 of ApoE gene and the haplotype GGC (rs7259620–rs1065853–rs439401) may be associated with the occurrence of EH in Guizhou Han population.

Association between Metabolic Score for Visceral Fat and the risk of hypertension in different ethnic groups: a prospective cohort study in Southwest China

“…One reason may be that different ethnic populations have unique genetic characteristics for the same gene. The migration of ethnic groups is hindered by geographical barriers and influenced by culture, so there are different degrees of different food habits, cultural life, and genetic exchange between different peoples ( 32 ). Existing studies have shown that there are some genetic differences between Han and ethnic groups in Guizhou.…”