Relationship between estrogen receptor 1 gene polymorphisms and postmenopausal osteoporosis of the spine in Chinese women

Dp, Shang; Hy, Lian; Dp, Fu; Wu, Jiang; Ss, Hou; Jm, Lu

doi:10.4238/gmr.15028106

Cited by 23 publications

(16 citation statements)

References 10 publications

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“…Additionally, the importance of ERs on cortical and trabecular bone, and also in different bone cell types have been widely explored (42). Although the studied genetic polymorphism rs2234693 and rs9340799 in ESR1 and rs1256049 and rs4986938 were not associated with PAP in the present study, they were previously associated with many different bone conditions (28,43) and oral conditions (43)(44)(45). The genetic polymorphisms rs2234693 and rs9340799 in ESR1 have been largely studied.…”

Section: Discussionmentioning

confidence: 61%

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Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions

et al. 2020

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This study evaluated the association between polymorphisms in genes encoding estrogen receptors 1 (ESR1) and 2 (ESR2), vitamin D receptor (VDR) and in microRNA17 (which binds to ESR1 and VDR) with persistent apical periodontitis (PAP) after the endodontic treatment. We included 162 patients who completed endodontic treatment at least one year ago and presented apical periodontitis at the beginning of the root canal therapy. Clinical and radiographic exams were performed to evaluate the presence of PAP or healthy periradicular tissues (healed). Saliva samples were collected as a genomic DNA. The genotyping of ESR1 (rs2234693 and rs9340799), ESR2 (rs1256049 and rs4986938), VDR (rs739837 and rs2228570) and miRNA17 (rs4284505) were performed by real-time PCR. Chi-square test was used to the distribution of genotype and allele frequencies. Haplotype analysis was also performed. Eighty-nine patients were included in the “healed” group and 73 in the “PAP” group. No association was found between the allelic and genotypic polymorphisms studied and PAP (p>0.05). Haplotype analysis also did not demonstrated an association (p>0.05). In conclusion, the genetic polymorphisms in ESR1, ESR2, VDR and miRNA17 are not associated with PAP.

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Section: Discussionmentioning

confidence: 61%

“…The evidence linking these polymorphisms and its haplotype to bone mineral density (28,46). Also they were associated with postmenopausal osteoporosis (28,43). According to Rivadeneira et al (47), genetic polymorphisms in ESR2 alone and in interaction with ESR1 can also influence the risk of bone fracture in postmenopausal women.…”

Section: Discussionmentioning

confidence: 99%

Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions

et al. 2020

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“…It has been indicated that ESR1 contributes to optimal periosteal bone formation through osteoblast progenitors, and a lack of ESR1 in osteoblasts leads to compromised bone mass and strength in female mice . Furthermore, ESR1 polymorphisms have been linked to postmenopausal osteoporosis of the spine in Chinese women . In addition, ESR1 has been identified as a target of miRNAs in the pathogenesis of various diseases .…”

Section: Discussionmentioning

confidence: 99%

miR‐22 represses osteoblast viability with ESR1 presenting a direct target and indirectly inactivating p38 MAPK/JNK signaling

Chen

Zhang

Chen

et al. 2020

The Journal of Gene Medicine

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Background: MicroRNAs (miRs) hold critical implications in the modulation of osteogenesis. This work was designed to unravel the underlying regulatory mechanism of miR-22 during osteoblast differentiation.Methods: Synthetic miR-22 mimics or inhibitors were transfected into preosteoblast MC3T3-E1 cells to regulate miR-22 expression. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and flow cytometry analyses were employed to assess cell proliferation and apoptosis. A quantitative real-time polymerase chain reaction and western blot assays were applied to measure mRNA and protein expression. Alkaline phosphatase activity and alizarin red staining were tested to further analyze cell differentiation. In silico analysis and luciferase reporter assays were utilized to identify the direct binding between miR-22 and its potential target.Results: MTT and flow cytometry analyses showed that miR-22 repressed MC3T3-E1 cell viability and promoted cell apoptosis. By detecting osteogenicspecific molecule expression, alkaline phosphatase activity and alizarin red staining, miR-22 was observed to suppress osteogenic differentiation of MC3T3-E1 cells. In silico analysis and luciferase reporter assays confirmed that ESR1 is a direct target gene of miR-22. In addition, miR-22 expression affected the phosphorylation of p38 mitogen-activated protein kinase and Jun N-terminal kinase expression in MC3T3-E1 cells. Conclusions:The findings of the present study highlight the functional significance of miR-22 in osteoblast differentiation and suggest its role as a possible therapeutic target in metabolic bone disorders. K E Y W O R D SESR1, miR-22, osteoblast differentiation, p38 MAPK/JNK signaling pathway Chen and Zhang contributed equally to this work.

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“…ESR1 and ESR2 genes encode estrogen receptors, involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis [ 22 ]. ESR1 is expressed in osteoblasts and osteoclasts, and is associated with postmenopausal osteoporosis of the spine in women [ 22 , 23 ]. This gene could be employed as a selection method to identify individuals at increased risk of osteoporosis [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…ESR1 is expressed in osteoblasts and osteoclasts, and is associated with postmenopausal osteoporosis of the spine in women [ 22 , 23 ]. This gene could be employed as a selection method to identify individuals at increased risk of osteoporosis [ 23 ]. In a previous large population-based cohort study, variants in the ESR2 gene were associated with an increased risk of vertebral fracture in postmenopausal women [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

A Network Pharmacology Approach to Determine the Active Components and Potential Targets of Curculigo Orchioides in the Treatment of Osteoporosis

Wang¹,

Zhao²,

Zhang³

et al. 2017

Med Sci Monit

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BackgroundOsteoporosis is a complex bone disorder with a genetic predisposition, and is a cause of health problems worldwide. In China, Curculigo orchioides (CO) has been widely used as a herbal medicine in the prevention and treatment of osteoporosis. However, research on the mechanism of action of CO is still lacking. The aim of this study was to identify the absorbable components, potential targets, and associated treatment pathways of CO using a network pharmacology approach.Material/MethodsWe explored the chemical components of CO and used the five main principles of drug absorption to identify absorbable components. Targets for the therapeutic actions of CO were obtained from the PharmMapper server database. Pathway enrichment analysis was performed using the Comparative Toxicogenomics Database (CTD). Cytoscape was used to visualize the multiple components-multiple target-multiple pathways-multiple disease network for CO.ResultsWe identified 77 chemical components of CO, of which 32 components could be absorbed in the blood. These potential active components of CO regulated 83 targets and affected 58 pathways. Data analysis showed that the genes for estrogen receptor alpha (ESR1) and beta (ESR2), and the gene for 11 beta-hydroxysteroid dehydrogenase type 1, or cortisone reductase (HSD11B1) were the main targets of CO. Endocrine regulatory factors and factors regulating calcium reabsorption, steroid hormone biosynthesis, and metabolic pathways were related to these main targets and to ten corresponding compounds.ConclusionsThe network pharmacology approach used in our study has attempted to explain the mechanisms for the effects of CO in the prevention and treatment of osteoporosis, and provides an alternative approach to the investigation of the effects of this complex compound.

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Relationship between estrogen receptor 1 gene polymorphisms and postmenopausal osteoporosis of the spine in Chinese women

Cited by 23 publications

References 10 publications

Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions

Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions

miR‐22 represses osteoblast viability with ESR1 presenting a direct target and indirectly inactivating p38 MAPK/JNK signaling

A Network Pharmacology Approach to Determine the Active Components and Potential Targets of Curculigo Orchioides in the Treatment of Osteoporosis

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