Relationship between <i>Verticillium dahliae</i> inoculum and sunflower wilt in Argentina

Erreguerena, Ignacio Antonio; Rojo, Rodrigo Nieto; Quiróz, Facundo; Clemente, G.E.; Escande, A. R.

doi:10.1080/07060661.2019.1620860

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…The breakage of resistances and the appearance of new Vd pathogenic races have underscored the need for BSR sources in sunflower [12][13][14]27,40 . The inoculum load measured in the infested field of EEA INTA Balcarce (circa 900 CFU/g, Supplementary Table 7), which is known to harbor a wide diversity of Vd isolates, is four times higher than the reported by Erreguerena et al (2019) 41 . In that report, 70 to 260 CFU/g rendered SVW-DI levels from 40% to 60%.…”

Section: Discussionmentioning

confidence: 60%

“…The inoculum titter at EEA-INTA Balcarce's evaluation field has been incremented by monocropping sunflower susceptible cultivars since 1997 and by soil tillage with crop debris. The Vd inoculum titter of EEA-INTA Balcarce's infested field was estimated around 900 CFU per gram of soil in season 2015/16 (Supplementary Table S7), according to Erreguerena et al (2019) 41 . El Cencerro's infested field is regularly used for testing sunflower commercial hybrids for SVW-resistance, along with susceptible testers.…”

Section: Field-trials and Experimental Designmentioning

confidence: 99%

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On-field Phenotypic Evaluation of Sunflower Germplasm: Breeding for Broad-spectrum Resistance to Verticillium Leaf Mottle and Wilt

Montecchia

Fass

Cerrudo

et al. 2021

Preprint

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Sunflower Verticillium Wilt and Leaf Mottle (SVW), caused by Verticillium dahliae (Kleb.; Vd), is a soil-borne disease affecting sunflower worldwide. A single dominant locus, known as V1, was formerly effective in controlling North-American Vd races, whereas races from Argentina, Europe and an emerging race from USA overcome its resistance. This emphasizes the need for identifying broad-spectrum genetic resistance (BSR) sources. Here we characterize two sunflower Mapping Populations (MPs) for SVW resistance: a biparental MP and the association MP from the National Institute of Agricultural Technology (INTA), under field growing conditions. Nine field-trials (FTs) were conducted in highly infested fields in the most SVW-affected region of Argentina. Several disease descriptors (DDs), including incidence and severity, were scored across four phenological stages. Generalized linear models were fitted according to the nature of each variable, adjusting mean phenotypes for inbred lines (IL) across and within FTs. Comparison of these responses allowed the identification of novel BSR sources. Furthermore, we present the first report of SVW resistance heritability, with estimates ranging from 35% to 45% for DDs related to disease incidence and severity, respectively. This study constitutes the largest SVW resistance characterization reported to date in sunflower, identifying valuable genetic resources for BSR-breeding to cope with a pathogen of increasing importance worldwide.

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Section: Discussionmentioning

confidence: 60%

Section: Field-trials and Experimental Designmentioning

confidence: 99%

On-field Phenotypic Evaluation of Sunflower Germplasm: Breeding for Broad-spectrum Resistance to Verticillium Leaf Mottle and Wilt

Montecchia

Fass

Cerrudo

et al. 2021

Preprint

View full text Add to dashboard Cite

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“…In the case of sunflower, V . dahliae is one of the most important pathogens and is widespread in the USA, Argentina, Europe (Pegg and Brady, 2002; Gulya et al ., 2019; Molinero‐Ruiz, 2019), and some regions of Canada (Erreguerena et al ., 2019). The only control measure available in sunflower is genetic resistance (Pegg and Brady, 2002), which was first reported in this crop in Manitoba in 1957 (Putt, 1958) and was found to be race specific and based on single genes (Fick and Zimmer, 1974).…”

Section: Introductionmentioning

confidence: 99%

Evidence of soil‐located competition as the cause of the reduction of sunflower verticillium wilt by entomopathogenic fungi

et al. 2020

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The increasing limitation of agrochemicals for disease control is a major challenge for European agriculture and a spur to developing environmentally friendly approaches such as biological control. Entomopathogenic fungi, which have been used in the control of insect pests for a long time, also have other uses, such as being antagonists of fungi, including plant pathogens. We determined the in vitro effect of three strains of Metarhizium brunneum and two of Beauveria bassiana against Verticillium dahliae and Cadophora helianthi, causal agents of sunflower wilts. Both M. brunneum and B. bassiana were able to inhibit the mycelial growth of the sunflower pathogens and, according to the dual culture and microscopy results, two types of antagonism were observed as being dependent on the strain: competition and/or antibiosis. Greenhouse experiments showed that, after soil treatments with entomopathogens and plant inoculation by root immersion in conidial suspensions of V. dahliae, the entomopathogens were able to efficiently persist in the soil, and three of the four strains even significantly reduced the severity of symptoms in sunflowers. Interestingly, molecular analysis showed that all the strains were able to establish themselves as endophytes in sunflowers in the absence of V. dahliae. When the plants were inoculated with V. dahliae, we detected the pathogen, but not the entomopathogen, in the sunflowers by molecular methods. The results of this work suggest that the protection conferred by M. brunneum and B. bassiana against verticillium wilt might not be plant‐located, but is probably the consequence of their competition with V. dahliae in the soil.

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Different appearance period of Verticillium wilt symptoms affects sunflower growth and production

Wang

Ning

et al. 2021

J Plant Pathol

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Relationship between Verticillium dahliae inoculum and sunflower wilt in Argentina

Cited by 4 publications

References 17 publications

On-field Phenotypic Evaluation of Sunflower Germplasm: Breeding for Broad-spectrum Resistance to Verticillium Leaf Mottle and Wilt

On-field Phenotypic Evaluation of Sunflower Germplasm: Breeding for Broad-spectrum Resistance to Verticillium Leaf Mottle and Wilt

Evidence of soil‐located competition as the cause of the reduction of sunflower verticillium wilt by entomopathogenic fungi

Different appearance period of Verticillium wilt symptoms affects sunflower growth and production

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