Relationship between interleukin-1-alpha concentration in tumors and cell growth in gastric cancer, determined using flow cytometry

Abstract

BACKGROUND: Interleukin-1-alpha (IL-1alpha) produced by tumor cells stimulates the proliferation or the growth of several cancer cell lines. The aim of this study was to clarify the relationship between growth activity evaluated by DNA analysis and the concentration of tumor-derived IL-1alpha in gastric cancers in clinical cases.METHODS: We measured the concentration of IL-1alpha in homogenized tumor samples obtained from 49 patients with gastric cancer, using an enzyme-linked immunosorbent assay, and we analyzed the cellular DNA content of paraffin-embedded tumor sections using flow cytometry.RESULTS: Both the IL-1alpha concentration and the percentage of S-phase fraction were significantly correlated with liver metastasis, histologic type, pattern of tumor infiltration, quantity of stroma, and venous invasion. A good correlation was found between IL-1alpha concentration in tumors and the percentage of S-phase fraction ( R = 0.604, P < 0.0001).CONCLUSION: IL-1alpha may be related to the stimulation of cell growth of gastric cancer in clinical cases.

Essential Role of Matrix Metalloproteinases in Interleukin-1-induced Myofibroblastic Activation of Hepatic Stellate Cell in Collagen

“…For example, systemic inflammation by injection of IL-1␣ is shown to enhance the metastasis of melanoma into the liver (52). A higher level of IL-1␣ was observed in colorectal cancer from patients with liver metastasis than in those without liver metastasis (53,54). We speculate that the paradigm shown by the current study for IL-1-mediated MMP-9 induction and activation by HSC may also contribute to metastasis of tumors including hepatocellular carcinomas.…”

Polymorphisms in Inflammation-related Genes and Risk of Gastric Cancer (Finland)

“…Selection of polymorphisms in this study was based on biologic properties of the genes and previously published literature. IL-1s are a cluster of genes whose products have an important role in controlling inflammatory reactions, as well as controlling the acidity of the stomach [7,8,28]. To our knowledge, only one previous study has examined the association between IL-1A À889 polymorphisms and GC and found no association [17].…”

Interleukin-1α Enhances Angiogenesis and Is Associated With Liver Metastatic Potential in Human Gastric Cancer Cell Lines

“…We previously evaluated the liver-metastatic ability of several gastric can- cer cell lines via intrasplenic liver metastasis assay and classified them as high liver-metastatic (MKN74) or low liver-metastatic (MKN45 and NUGC-4) [29]. Recent research has demonstrated that IL-1␣ contributes to the regulation of tumor growth, progression, and liver metastasis in primary gastric carcinoma [12,30,31]. In the present study, we demonstrated that IL-1␣ mRNA and protein were detected only in the high liver-metastatic gastric cancer cell line MKN74, but not in the low liver-metastatic cell lines MKN45 or NUGC-4.…”