Relationship between intestinal flora and eczema in term infants by Shotgun sequencing

Abstract

Eczema is one of the common skin diseases in the neonatal period. The purpose of this study was to use Shotgun sequencing technology to analyze and compare the main components of the intestinal flora in the feces of eczema term infants and healthy term infants, and to explore the correlation between the characteristics of the intestinal flora and the incidence of eczema in term infants. There were 10 full-term infants who met the inclusion criteria in the Obstetrics Department of the First People's Hospital of Yancheng City, including 5 cases in the eczema group (group E) and 5 cases in the control group (group C). The fecal samples of the 10 full-term infants were collected at five time points: 0 days (meconium), 7 days, 14 days, 21 days, and 28 days, and the DNA of the fecal samples was extracted. According to the EASI scoring method, the eczema area and severity index of the eczema group were evaluated. The above 50 stool samples were subjected to high-throughput metagenomic sequencing of gut microbiota using Shotgun sequencing technology. SPSS 24.0 software was used to analyze the dynamic changes of Staphylococcus epidermidis, Escherichia coli and Bifidobacterium in feces from 0-28 days and the distribution difference of intestinal flora between the eczema group and the control group, and further explore the content of the above bacteria in the eczema group Correlation with eczema severity. The content and distribution characteristics of Staphylococcus epidermidis, Escherichia coli and Bifidobacterium in the intestinal flora of term infants have a certain correlation with the pathogenesis of eczema.