Relationship between light intensity and chlorophyll content in Myriophyllum spicatum L. in Canadice Lake (New York, U.S.A.)

Marcus, Bernard

doi:10.1016/0304-3770(80)90017-0

Cited by 19 publications

(9 citation statements)

References 3 publications

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“…c Marwood et al [20] had an n ϭ 2 with four subsamples averaged per outdoor microcosm. Studies of natural M. spicatum communities in North America found that chlorophyll a and b concentrations varied greatly between plants, with no relationship between pigment concentration and depth of the plant [43], thus implying a large, naturally inherent amount of variation in these measures. e Snel et al [39] had an n ϭ 2 using indoor microcosms.…”

Section: Discussionmentioning

confidence: 99%

Variation, Replication, and Power Analysis of Myriophyllum Spp. Microcosm Toxicity Data

Hanson¹,

Sanderson²,

Solomon³

2003

Environ Toxicol Chem

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Myriophyllum spp. have been proposed as a new standard laboratory aquatic macrophyte test species for the registration of pesticides. The main objectives of this investigation were to determine the power of Myriophyllum sibiricum and Myriophyllum spicatum toxicity data derived from an outdoor microcosm bioassay, to evaluate the variation of 10 different aquatic plant endpoints and to calculate the minimum detectable difference for these endpoints, to determine the replication required to detect ecologically significant changes from control for these endpoints, and to make recommendations for future field studies with Myriophyllum spp. Control data from four different studies that characterized haloacetic acid toxicity with Myriophyllum spp. for durations of three to six weeks during the summer of 1999 with five treatment levels (n = 3), including control, were examined. Endpoint coefficient of variation ranged, on average, from 6 to 28%. Node number and plant length endpoints were consistently the most statistically powerful for both plant species. It was possible to detect approximately 30% change from control in both endpoints with high statistical power (beta = 0.2, alpha = 0.05, n = 3). The range of minimum detectable differences was 40 to 60% for the other endpoints. Replication to detect a > or = 25% change from control would require an n of 2 to 21, depending on the endpoint. Myriophyllum sibiricum had slightly lower coefficients of variation and thus required fewer replicates than M. spicatum to be statistically significantly different from control values. Variation within microcosm studies was not significantly different from that of controlled laboratory studies, implying that most of the variation observed in the field is inherent in the plants. Based on statistical sensitivity, ecological relevance, and toxicological sensitivity, we recommend using plant length and root endpoints as indicators of toxicity under field conditions.

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Section: Discussionmentioning

confidence: 99%

Variation, Replication, and Power Analysis of Myriophyllum Spp. Microcosm Toxicity Data

Hanson¹,

Sanderson²,

Solomon³

2003

Environ Toxicol Chem

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“…Marcus (1980) detailed the procedures for chlorophyll determination in aquatic plants using acetone as an extracting solvent. Since then, the acetone method for chlorophyll extraction in aquatic plants has been widely used.…”

Section: Introductionmentioning

confidence: 99%

Optimization of the Method for Chlorophyll Extraction in Aquatic Plants

Su¹,

Zhou²,

Qin³

et al. 2010

Journal of Freshwater Ecology

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We compared the common methods of chlorophyll measurement in Lemna minor (duckweed), Potamogeton crispus, and Egeria densa in an attempt to identify an optimal protocol to quickly measure chlorophyll in aquatic plants. Three physical processes including grinding-settling (GS), grinding-elution (GE) and immersing (IM) were compared for chlorophyll extraction in 80% acetone with duckweed. Then, the GE method was chosen for tissue rupture, and the efficiency of three solvents (80% aqueous acetone, 90% aqueous acetone and 95% aqueous ethanol) on chlorophyll extraction was tested on duckweed, P. crispus, and E. densa. There was no difference in the amount of chlorophyll extracted between the procedures of GE and GS, or between GE and IM. The amount of total chlorophyll extracted by 90% acetone was higher than that extracted by 80% acetone or 95% ethanol in the three aquatic plants. Furthermore, the chlorophyll extracted by and held in 90% acetone was more stable over time than it was with either 80% acetone or 95% ethanol. Therefore, the procedure of grinding-elution for physical rupture and 90% acetone for extraction is recommended as a quick and reliable protocol for chlorophyll extraction in aquatic plants.

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“…Although there has been much controversy about the factors which determine the composition of epiphytic community (Gough & Woelkerling, 1976;Cattaneo & Kalff, 1979, 1980Aloi, 1990;Cattaneo & Amireault, 1992;Cattaneo et al, 1998;Jones et al, 2000), this study, nonetheless, showed the importance of a multiscale approach in observing epiphytic patterns.…”

Section: Discussionmentioning

confidence: 90%

“…These results corroborated those published by Wetzel (1983), Watanabe et al (1988) and Tesolin & Tell (1996), who observed a great stability in a common minority among the strict epiphyton. Two of these species are regularly described in those studies because they are able to colonize quickly and have a high capacity of migration (Pringle, 1990;Acs & Kiss, 1993a, b): C. pediculus is known as a superior competitor (Dickman & Gochnauer, 1978;Marcus, 1980) and A. minutissima is commonly dominant as well, due to its highly adaptative capacity to secrete different means of fixation, depending on the strength of the current (Acs & Buczko, 1994). A. minutissima has an adnate form, as we found here in the Sorgue river, (when current >1 m s )1 ) and secretes a long stalk when the current is lower.…”

Section: Discussionmentioning

confidence: 97%

“…Many authors tend to consider macrophytes as neutral substrate (Shelford, 1918;Harlin, 1973;Carignan & Kalff, 1982;Cattaneo & Kalff, 1978, 1979, 1980Fontaine & Nigh, 1983;Millie & Lowe, 1983;Morin, 1986), thus providing an explanation for the observation that artificial plants and natural plants display the same epiphytic communities (Cattaneo & Kalff, 1979;Gough & Gough, 1981). In contrast, several studies have shown that plants release various nutrients (Allen, 1971;McRoy & Goering, 1974;Pip & Stewart, 1976) and active chemicals from different parts of their tissues (Anthoni et al, 1980;Planas et al, 1981;Wium-Andersen et al, 1983Burkholder & Wetzel, 1989;Pip, 1992;Gross et al, 1996).…”

Section: Introductionmentioning

confidence: 90%

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Quantitative and qualitative variability of epiphytic algae on one Apiaceae (Apium nodiflorum L.) in a karstic river (Southeast of France)

Comte

Fayolle²,

Roux³

2005

Hydrobiologia

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Spatial distribution of the firmly attached epiphytic algae was investigated directly on the leaves of one Apiaceae (Apium nodiflorum L.) commonly found in chalky Mediterranean rivers, at two sampling dates (summer and autumn). The composition and structure of the epiphytic communities of the samples were determined at different levels: along the leaves, on both their sides and within each leaflet (n ¼ 13). Forty-eight leaflets from four randomly selected plants were cut along the longitudinal and vertical axes so as to define 16 microhabitats located on the edge, the centre and the vein. Only a few species of algae were found to be constantly present whatever the sampling date, and dominated all microzones (n ¼ 768). Their presence seemed to depend, rather, on the architectural structure of the plant. The other algae represented the major part of the epiphytic community and were only occasionally present. Those were more subject to temporal and environmental variations and less specific of the substrate. The epiphytes showed considerable spatial heterogeneity within their microhabitats, with an evident preference for the centre and the edge of the leaves, whereas two inhospitable spaces were the vein and apex. On the other hand, when the study was performed at a larger scale (along the leaf), the epiphytic populations of the youngest leaves (near the surface) and the eldest ones (close to the sediments) were homogenous, hence suggesting that other factors, independent from the physiology of the plant, may have an impact on epiphytic distribution.

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Relationship between light intensity and chlorophyll content in Myriophyllum spicatum L. in Canadice Lake (New York, U.S.A.)

Cited by 19 publications

References 3 publications

Variation, Replication, and Power Analysis of Myriophyllum Spp. Microcosm Toxicity Data

Variation, Replication, and Power Analysis of Myriophyllum Spp. Microcosm Toxicity Data

Optimization of the Method for Chlorophyll Extraction in Aquatic Plants

Quantitative and qualitative variability of epiphytic algae on one Apiaceae (Apium nodiflorum L.) in a karstic river (Southeast of France)

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