Relationship between matrix metalloproteinase expression and tumor angiogenesis in human breast carcinoma.

Kurizaki, Takashi; Toi, Masakazu; Tominaga, Takeshi

doi:10.3892/or.5.3.673

Cited by 38 publications

(38 citation statements)

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“…One possible explanation for the finding that bombesin/GRP antagonists affect MMP-2 and MMP-9 differently is that MMP-2 does not contain AP-l-binding sites in its promoter, but MMP-9 gene harbours an AP-1-binding site in the proximal promoter (Nagase and Woessner, 1999) bombesin/GRP antagonists were also accompanied by a significant reduction in the vessel density of MDA-MB 435 breast tumours. This is in accord with earlier findings that the expression of pro-MMP-9 and the microvascular density may be related in human breast carcinoma (Kurizaki et al, 1998).…”

Section: Discussionsupporting

confidence: 93%

“…Thus, there is a significant correlation between activated MMP-2 expression and VEGF expression, and similarly pro-MMP-9 expression tends to correlate with the increment of MVD (Kurizaki et al, 1998). IGF-II has also been described as an angiogenic factor that acts either directly (Volpert et al, 1996;Lee et al, 2000) or indirectly through an increase in VEGF production and the upregulation of the expression of proteolytic enzyme MMP-2 (Kim et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

“…These MMPs may also play a role in tumour vascularisation. Thus, it has been shown that the expression of MMP-2 correlates with VEGF and IGF-II expression, and that of MMP-9 with bFGF and increased microvessel density (MVD) (Kurizaki et al, 1998;Lee et al, 2000).…”

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confidence: 99%

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Bombesin antagonists inhibit proangiogenic factors in human experimental breast cancers

et al. 2004

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The overexpression of angiogenic factors such as vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF) and insulin-like growth factors (IGFs) plays a role in the migration and proliferation of endothelial cells in many cancers. Consequently, we investigated the effects of bombesin/gastrin-releasing peptide (GRP) antagonists on the expression of these angiogenic factors, the activities of matrix metalloproteinases (MMPs)-2 and -9, as well as the vascular density in MDA-MB-435 human oestrogenindependent breast cancers. Nude mice bearing orthotopic xenografts of MDA-MB-435 breast cancers were treated with bombesin/ GRP antagonists for 6 weeks. Daily administration of 20 mg of RC-3095 or 10 mg of RC-3940-II significantly decreased the weight of MDA-MB-435 cancers by 44 and 53%, respectively. The inhibition of tumour growth was associated with a substantial reduction in the expression of mRNA and protein levels of basic fibroblast growth factor (bFGF), IGF-II and VEGF-A in the tumours. Both bombesin/GRP antagonists significantly decreased the vessel density of the tumours by about 37%, as shown by immunohistochemical detection of vessels on tumour slides. Gelatinolytic activities, detected by zymography, revealed a 33 -46% reduction in MMP-9 activity after the treatment with either antagonist. In vitro studies revealed that MDA-MB-435 cells secrete bFGF, IGF-II and VEGF-A, and the secretion of these factors is inhibited by RC-3095 and RC-3940-II. This study demonstrates the antiangiogenic effect of bombesin/GRP antagonists RC-3095 and RC-3940-II, and underscores their possible therapeutic application for treatment of breast cancers.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Bombesin antagonists inhibit proangiogenic factors in human experimental breast cancers

et al. 2004

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“…Under normal physiological conditions, the MMPs and their endogenous inhibitors, the tissue inhibitors of metalloproteinases, exist in an exquisite balance. Unbalanced MMP/ tissue inhibitors of metalloproteinase expression plays a critical role in cellular movement, invasion, angiogenesis, and metastasis (5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

A Randomized Phase II Feasibility Trial of BMS-275291 in Patients with Early Stage Breast Cancer

Miller

Saphner

Waterhouse

et al. 2004

Clinical Cancer Research

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“…MMPs are enzymes that are essential for this process. MMPs play a role in the degradation of the extracellular matrix by promoting epithelial-mesenchymal transition, promoting the activation of growth factors, their receptors and angiogenesis, and facilitating tumor invasion and metastasis (24)(25)(26)(27). Previous studies revealed that the expression of MMP-2 in breast cancer tissue is higher compared with normal breast tissue.…”

Section: Discussionmentioning

confidence: 99%

Expression of WTH3 in breast cancer tissue and the effects on the biological behavior of breast cancer cells

Gan

Zuo

Wang

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. The aim of the present study was to investigate the expression of WTH3 in tumor and normal breast tissue. The mRNA and protein expression levels of WTH3 were detected using reverse transcription quantitative polymerase chain reaction and western blot analysis, respectively. In addition, matrix metalloproteinase (MMP)-2 protein expression was measured. The effect of WTH3 expression on the proliferation activity of breast cancer cells was detected using a Cell Counting Kit-8 assay. Furthermore, the effects of WTH3 on the invasion and migration ability of the breast cancer cells was investigated. The results revealed that WTH3 was able to significantly inhibit the proliferation of the MCF-7 and MDA-MB-231 breast cancer cell lines. In addition, the invasion and migration assay demonstrated that WTH3 was able to inhibit the invasion and migration of breast cancer cells. Western blot analysis revealed that increased expression of WTH3 resulted in decreased expression levels of MMP-2, which has an important function in the metastasis of cancer cells. In conclusion, WTH3 expression differed between the tumor and normal breast tissues. WTH3 was able to inhibit the proliferation of breast cancer cells and decrease their invasion ability. Thus, WTH3 may be a promising target for breast cancer therapy in the future.

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Relationship between matrix metalloproteinase expression and tumor angiogenesis in human breast carcinoma.

Cited by 38 publications

References 0 publications

Bombesin antagonists inhibit proangiogenic factors in human experimental breast cancers

Bombesin antagonists inhibit proangiogenic factors in human experimental breast cancers

A Randomized Phase II Feasibility Trial of BMS-275291 in Patients with Early Stage Breast Cancer

Expression of WTH3 in breast cancer tissue and the effects on the biological behavior of breast cancer cells

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