Relationship Between Metabolites Composition and Biological Activities of Phyllanthus niruri Extracts Prepared by Different Drying Methods and Solvents Extraction

Mediani, Ahmed; Abas, Faridah; Khatib, Alfi; Tan, Chin Ping; Ismail, Intan Safinar; Shaari, Khozirah; Ismail, Amin; Lajis, Nordin Hj

doi:10.1007/s11130-015-0478-5

Cited by 31 publications

(17 citation statements)

References 28 publications

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“…Previously, Lin et al [33] found that there were insignificant differences in the α-glucosidase activity of Rabdosia serra leaf prepared by AD and SD methods. While Mediani et al [13] reported that AD extracts of Phyllanthus niruri exhibited higher α-glucosidase activity compared to OD α-GLUCOSIDASE INHIBITORY ACTIVITY OF IPOMOEA AQUATICA extracts. Differences in our findings and previous reports are probably due to differences in plant composition (i.e., metabolites, water content, and cell structure).…”

Section: Effect Of Different Drying Treatments and Solvents On Tpc Anmentioning

confidence: 97%

“…Previously, PLS has been successfully used to correlate the bioactivity with phytochemical constituents. [13,14] In PLS, the data are separated into two blocks, the X-block represents the predictor variables (NMR chemical shift of the metabolites) and the Y-block represents the response variables (bioactivity). [40] By using PLS, the relationship between bioactivities (1/IC 50 of α-glucosidase inhibitory and TPC) and the metabolites in the ethanol extracts can be established.…”

Section: Classification Of I Aquatica Extracts In Different Drying Mmentioning

confidence: 99%

“…It is well known that recovery of bioactive metabolites from plant extracts depend largely on the efficiency of the extraction process and the composition of the metabolites in the plant cell matrix. [13] Treatments such as heat, extraction solvents, solvent-to-sample ratio, and time of the extraction have profound effect on the extractable yield of bioactive metabolites. Drying is a process that eliminates excess moisture to avoid spoilage and to extent the shelf life of food.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Different Drying Treatments and Solvent Ratios on Phytochemical Constituents of Ipomoea aquatica and Correlation with α-Glucosidase Inhibitory Activity

Sajak

Abas

Ismail

et al. 2016

International Journal of Food Properties

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Ipomoea aquatica is an aquatic plant that is widely consumed in Southeast Asia as a vegetable. In this study, the influence of various ethanol ratios (0, 20, 50, 80, and 100%) as an extraction solvent and different drying methods including air drying, sun drying, and oven drying on phytochemical constituents of I. aquatica was investigated using a proton nuclear magnetic resonance-based metabolomics approach. The effect on α-glucosidase inhibitory activity and total phenolic content was also examined. Clear discrimination was observed between different ethanol ratios and different drying processes by principal component analysis. The highest α-glucosidase inhibitory activity was observed for absolute ethanol extract from the oven drying method with IC 50 value of 204.0 ± 59.0 µg/mL and total phenolic content value of 22.0 ± 0.7 µg gallic acid equivalent/mg extract. Correlation between the α-glucosidase inhibitory activity and the metabolite were analyzed using a partial least square analysis. The metabolites that are responsible for the activity were quercetin derivatives, chlorogenic acid derivatives, sucrose, and fructose. This study highlights the basis for future investigations of I. aquatica as a source of food that has the potential for nutraceutical enhancement and as ingredient in medicinal preparation.

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Section: Effect Of Different Drying Treatments and Solvents On Tpc Anmentioning

confidence: 97%

Section: Classification Of I Aquatica Extracts In Different Drying Mmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Different Drying Treatments and Solvent Ratios on Phytochemical Constituents of Ipomoea aquatica and Correlation with α-Glucosidase Inhibitory Activity

Sajak

Abas

Ismail

et al. 2016

International Journal of Food Properties

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“…Regarding phenolic compounds, mainly flavonoids such as kaempferol derivatives have been reported in S. reticulata [2] [7]; quercetin derivatives in P. alliacea [8] and P. niruri [9]; whereas caffeic acid derivatives and ellagitannins have also been reported in P. niruri [10]. Flavan-3-ols, including catechin and epicatechin monomers have been reported in P. niruri [11], whereas no detailed studies on proanthocyanidin oligomers have been performed in any of these three species.…”

Section: Introductionmentioning

confidence: 99%

Proanthocyanidin Characterization, Antioxidant and Cytotoxic Activities of Three Plants Commonly Used in Traditional Medicine in Costa Rica: Petiveria alliaceae L., Phyllanthus niruri L. and Senna reticulata Willd.

Navarro

Moreira

Arnáez

et al. 2017

Plants

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The phenolic composition of aerial parts from Petiveria alliaceae L., Phyllanthus niruri L. and Senna reticulata Willd., species commonly used in Costa Rica as traditional medicines, was studied using UPLC-ESI-TQ-MS on enriched-phenolic extracts. Comparatively, higher values of total phenolic content (TPC), as measured by the Folin-Ciocalteau method, were observed for P. niruri extracts (328.8 gallic acid equivalents/g) than for S. reticulata (79.30 gallic acid equivalents/g) whereas P. alliaceae extract showed the lowest value (13.45 gallic acid equivalents/g). A total of 20 phenolic acids and proanthocyanidins were identified in the extracts, including hydroxybenzoic acids (benzoic, 4-hydroxybenzoic, gallic, prochatechuic, salicylic, syringic and vanillic acids); hydroxycinnamic acids (caffeic, ferulic, and p-coumaric acids); and flavan-3-ols monomers [(+)-catechin and (−)-epicatechin)]. Regarding proanthocyanidin oligomers, five procyanidin dimers (B1, B2, B3, B4, and B5) and one trimer (T2) are reported for the first time in P. niruri, as well as two propelargonidin dimers in S. reticulata. Additionally, P. niruri showed the highest antioxidant DPPH and ORAC values (IC50 of 6.4 μg/mL and 6.5 mmol TE/g respectively), followed by S. reticulata (IC50 of 72.9 μg/mL and 2.68 mmol TE/g respectively) and P. alliaceae extract (IC50 >1000 μg/mL and 1.32 mmol TE/g respectively). Finally, cytotoxicity and selectivity on gastric AGS and colon SW20 adenocarcinoma cell lines were evaluated and the best values were also found for P. niruri (SI = 2.8), followed by S. reticulata (SI = 2.5). Therefore, these results suggest that extracts containing higher proanthocyanidin content also show higher bioactivities. Significant positive correlation was found between TPC and ORAC (R2 = 0.996) as well as between phenolic content as measured by UPLC-DAD and ORAC (R2 = 0.990). These findings show evidence for the first time of the diversity of phenolic acids in P. alliaceae and S. reticulata, and the presence of proanthocyanidins as minor components in latter species. Of particular relevance is the occurrence of proanthocyanidin oligomers in phenolic extracts from P. niruri and their potential bioactivity.

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“…Originally, this herb contains chemical substances including lignin, glycoside, alkaloid, ellagitannin, terpenoid, phenylpropanoid, flavonoid and polyphenol [6]. Instead of this herb has been studied its pharmacological activity in the treatment of bladder infection, as immunomodulator, anti-inflammatory and antiviral agents [7,8], to date, there is no report about this species to be used as an endophyte source. Hence, it is interesting to study more about extraction and biological testing of fungal endophyte from Phyllanthus niruri.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial, antioxidant, and cytotoxic activities of endhopitic fungi Chaetomium sp. isolated from Phyllanthus niruri Linn: in vitro and in silico studies

Rollando

Notario

Monica

et al. 2017

J. Pure App. Chem. Res.

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Endophytic fungi Chaetomium sp isolated from Phyllanthus niruri Linn. Mycelium powder was extracted by using ethyl acetate. Extract was fractionated using n-hexane, dichloromethane and ethanol 96%. The antimicrobial test was carried out using disc diffusion and microdilution methods. The antioxidant activity of the fraction was determined using hydrogen peroxide free radical scavenging and reducing power capacity activities. The cytotoxicity assay of the fraction against T47D breast cancer cell was carried out using dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide method (MTT). The in silico prediction of chemical substances which are reported exist in Chaetomium sp. performed using AutoDockVina embedded in PyRx version 8.0. Dichloromethane fraction was found as the most active sample against Escherichia coli (IC 50 20.76 g/mL), Staphylococcus aureus (IC 50 70.15 g/mL), Salmonella typhi (49.13 g/mL) and was found as the most high phenolic content with value 47.44 mg GAE/g fraction, whereas the best antioxidant activity was performed by ethanol 96% fraction (85%). Cytotoxicity assay against T47D cell line showed dichloromethane fraction have highest activity with IC 50 10.76 g/mL. The docking studies showed that compounds bearing xanthone structure were potential for maltose binding periplasmic and human aromatase associating with their potencies as antibacteria and anticancer. Endophytic fungi Chaetomium sp. was isolated from Phyllanthus niruri using n-hexane, dichloromethane and ethanol fractions was studied its various biological activities as antimicrobial, antioxidant and cytotoxic agent against breast cancer cell.

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Relationship Between Metabolites Composition and Biological Activities of Phyllanthus niruri Extracts Prepared by Different Drying Methods and Solvents Extraction

Cited by 31 publications

References 28 publications

Effect of Different Drying Treatments and Solvent Ratios on Phytochemical Constituents of Ipomoea aquatica and Correlation with α-Glucosidase Inhibitory Activity

Effect of Different Drying Treatments and Solvent Ratios on Phytochemical Constituents of Ipomoea aquatica and Correlation with α-Glucosidase Inhibitory Activity

Proanthocyanidin Characterization, Antioxidant and Cytotoxic Activities of Three Plants Commonly Used in Traditional Medicine in Costa Rica: Petiveria alliaceae L., Phyllanthus niruri L. and Senna reticulata Willd.

Antimicrobial, antioxidant, and cytotoxic activities of endhopitic fungi Chaetomium sp. isolated from Phyllanthus niruri Linn: in vitro and in silico studies

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