Relationship Between Motility and Mitochondrial Functional Status in Canine Spermatozoa

Volpe, Sara; Leoci, Raffaella; Aiudi, Giulio Guido; Lacalandra, Giovanni Michele

doi:10.1111/j.1439-0531.2009.01457.x

Cited by 32 publications

(29 citation statements)

References 13 publications

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“…Upon M. pruriens administration, we observed significant recovery of mitochondrial membrane potential coupled with a gain in sperm motility (Figure 3). The relationship between ROS and MMP, and between MMP and sperm motility has been reported in different species including humans [30], [31]. Infertility in humans presents a complex picture of disturbances at the hormone level, generation and disposal of ROS and the actual process of spermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

Mucuna pruriens and Its Major Constituent L-DOPA Recover Spermatogenic Loss by Combating ROS, Loss of Mitochondrial Membrane Potential and Apoptosis

et al. 2013

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BackgroundThe Ayurvedic medicinal system claims Mucuna pruriens (MP) to possess pro-male fertility, aphrodisiac and adaptogenic properties. Some scientific evidence also supports its pro-male fertility properties; however, the mechanism of its action is not yet clear. The present study aimed at demonstrating spermatogenic restorative efficacy of MP and its major constituent L-DOPA (LD), and finding the possible mechanism of action thereof in a rat model.Methodology/FindingsEthinyl estradiol (EE) was administered at a rate of 3 mg/kg body weight (BW)/day for a period of 14 days to generate a rat model with compromised spermatogenesis. MP and LD were administered in two separate groups of these animals starting 15th day for a period of 56 days, and the results were compared with an auto-recovery (AR) group. Sperm count and motility, testis histo-architecture, level of reactive oxygen species (ROS), mitochondrial membrane potential (MMP), apoptosis, peripheral hormone levels and testicular germ cell populations were analysed, in all experimental groups. We observed efficient and quick recovery of spermatogenesis in MP and LD groups in comparison to the auto-recovery group. The treatment regulated ROS level, apoptosis, and mitochondrial membrane potential (MMP), recovered the hypothalamic-pituitary-gonadal axis and the number of testicular germ cells, ultimately leading to increased sperm count and motility.Conclusion/Significance M. pruriens efficiently recovers the spermatogenic loss induced due to EE administration. The recovery is mediated by reduction in ROS level, restoration of MMP, regulation of apoptosis and eventual increase in the number of germ cells and regulation of apoptosis. The present study simplified the complexity of mechanism involved and provided meaningful insights into MP/LD mediated correction of spermatogenic impairment caused by estrogens exposure. This is the first study demonstrating that L-DOPA largely accounts for pro-spermatogenic properties of M. pruriens. The manuscript bears CDRI communication number 8374.

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Section: Discussionmentioning

confidence: 99%

Mucuna pruriens and Its Major Constituent L-DOPA Recover Spermatogenic Loss by Combating ROS, Loss of Mitochondrial Membrane Potential and Apoptosis

et al. 2013

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“…Interestingly, 5-HT immunoreactivity was located mainly in the midpiece, a region essential for oxidative metabolism and for promoting sperm motility (Volpe et al 2009). It seems that 5-HT could activate principal piece through a cAMP-dependent dynein phosphorylation process (Stephens & Prior 1992).…”

Section: Discussionmentioning

confidence: 99%

Evidence of 5-HT components in human sperm: implications for protein tyrosine phosphorylation and the physiology of motility

Jiménez-Trejo¹,

Tapia-Rodríguez²,

Cerbón³

et al. 2012

Reproduction

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Serotonin (5-hydroxytryptamine; C 10 H 12 N 2 O (5-HT)) is produced in the CNS and in some cells of peripheral tissues. In the mammalian male reproductive system, both 5-HT and tryptophan hydroxylase (TPH) have been described in Leydig cells of the testis and in principal cells of the caput epididymis. In capacitated hamster sperm, it has been shown that 5-HT promotes the acrosomal reaction. The aim of this work was to explore the existence of components of the serotoninergic system and their relevance in human sperm physiology. We used both immunocytochemistry and western blot to detect serotoninergic markers such as 5-HT, TPH1, MAO A , 5-HT 1B , 5-HT 3 , and 5HT T ; HPLC for TPH enzymatic activity; Computer Assisted Semen Analysis assays to measure sperm motility parameters and pharmacological approaches to show the effect of 5-HT in sperm motility and tyrosine phosphorylation was assessed by western blot. We found the presence of serotoninergic markers (5-HT, TPH1, MAO A , 5-HT 1B , 5-HT 2A , 5-HT 3 , 5-HT T , and TPH enzymatic activity) in human sperm. In addition, we observed a significant increase in tyrosine phosphorylation and changes in sperm motility after 5-HT treatment. In conclusion, our data demonstrate the existence of components of a serotoninergic system in human sperm and support the notion for a functional role of 5-HT in mammalian sperm physiology, which can be modulated pharmacologically.

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“…() challenged the previous understanding that oxidative phosphorylation is the dominant metabolic pathway for sperm motility in the stallion. Similar to Volpe and coworkers (Volpe et al., ), Davila et al. () used the MMP probe JC‐1 and computer‐assisted sperm motility analysis, but also included DNP and oligomycin as controls in their analysis of ejaculates from Spanish stallions.…”

Section: Assessing Sperm Mitochondriamentioning

confidence: 99%

Sperm mitochondrial regulation in motility and fertility in horses

Meyers

Bulkeley

Foutouhi

2019

Reprod Domestic Animals

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The biological nature of age‐related declines in fertility in males of any species, including stallions, has been elusive. In horses, the economic costs to the breeding industry are frequently extensive. Mitochondrial function in ejaculated sperm, which is essential for sperm motility, is reflected by adenosine triphosphate production, mitochondrial oxidative efficiency and production of reactive oxygen species, and that this balance may become compromised in ageing stallions and during the process of cryopreservation. This presentation will focus on mitochondrial integrity and function as an avenue for understanding the pathophysiology of sperm when undergoing cryopreservation and male ageing. We discuss the importance of understanding the differences and similarities of sperm mitochondria to that of somatic cells regarding structure and mitochondrial biochemistry relating to sperm function. The roles of oxidative phosphorylation and glycolysis in sperm mitochondria are outlined as is the method of determining oxygen consumption and calcium homoeostasis in sperm mitochondria. Further, we outline the role of oxidative stress and reactive oxygen species.

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Relationship Between Motility and Mitochondrial Functional Status in Canine Spermatozoa

Cited by 32 publications

References 13 publications

Mucuna pruriens and Its Major Constituent L-DOPA Recover Spermatogenic Loss by Combating ROS, Loss of Mitochondrial Membrane Potential and Apoptosis

Mucuna pruriens and Its Major Constituent L-DOPA Recover Spermatogenic Loss by Combating ROS, Loss of Mitochondrial Membrane Potential and Apoptosis

Evidence of 5-HT components in human sperm: implications for protein tyrosine phosphorylation and the physiology of motility

Sperm mitochondrial regulation in motility and fertility in horses

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