Relationship between NRAGE and the radioresistance of esophageal carcinoma cell line TE13R120

Xue, Xiaoying; Liu, Zhi-He; Jing, Feng-Min; Li, Yange; Liu, Huizhi; Gao, Xianshu

doi:10.5732/cjc.010.10141

Cited by 14 publications

(14 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A genome-wide association study demonstrated that the initiation of NRAGE signals through the JNK pathway is associated with non-small cell lung cancer (22,30). Xue et al (38) identified an association between elevated NRAGE expression and the increased radioresistance of esophageal carcinoma cells. Such studies indicate that NRAGE functions to inhibit or promote oncogenesis depending on cell type, leading to the rationale behind the present study.…”

Section: Discussionmentioning

confidence: 99%

NRAGE promotes the malignant phenotype of hepatocellular carcinoma

et al. 2016

View full text Add to dashboard Cite

Abstract. Hepatocellular carcinoma (HCC) is a fatal disease, primarily due to the limited effective therapies available for patients with advanced or recurrent stages of the disease. Therefore, in order to improve patient prognosis, it is important to identify an informative biomarker for HCC progression, as well as a molecular target for therapy. Neurotrophin receptor-interacting melanoma antigen-encoding protein (NRAGE), a member of the type II melanoma-associated antigen family, mediates apoptosis and cell death through interactions with a wide range of proteins, and is implicated as a tumor suppressor or oncoprotein depending on cell type. However, the role of NRAGE in HCC is currently unknown, therefore, the present study aimed to identify the underlying function of NRAGE in HCC tumorigenesis. Resected tumor and non-cancerous liver tissues from 151 patients with HCC, alongside HCC cell lines, were analyzed by polymerase chain reaction and immunohistochemical techniques to determine NRAGE expression levels, as well as the expression levels of potential genes encoding interacting proteins. It was demonstrated that the expression levels of NRAGE mRNA correlated significantly with those of apoptosis-antagonizing transcription factor (AATF), and were not affected by cirrhosis in non-cancerous liver tissues when compared to elevated levels in HCC tissues. The expression patterns of NRAGE protein and mRNA were consistent among 30 representative specimen pairs. Furthermore, increased NRAGE expression in patients with HCC correlated significantly with a shorter disease-specific survival time, and was identified as an independent prognostic factor via multivariate analysis (hazard ratio, 2.23; 95% confidence interval, 1.06-3.83; P=0.020). Therefore, the results of the present study indicated that increased NRAGE expression affects HCC progression via its interaction with AATF, and may represent a novel biomarker and molecular target for the treatment of HCC. IntroductionHepatocellular carcinoma (HCC) is one of the most prevalent malignant tumors, with high rates of mortality and morbidity (1,2). The worldwide incidence of HCC is ~1,000,000 cases per year and is equivalent to its mortality rate (3). Furthermore, HCC ranks as the third highest cause of cancer-associated mortality (4). Such statistics are largely explained by the difficulties faced when diagnosing HCC, with diagnosis often only confirmed once the disease is at a late stage and palliative care is the only treatment option available. An important strategy to improve patient outcome involves the identification of disease risk factors and the maintenance of vigilant surveillance of high-risk individuals to allow for recognition of the disease at a stage that is responsive to treatment with curative intent. Therefore, it is fundamental that high-risk groups are identified, followed by successful implementation of a surveillance program and recall protocol following any abnormal findings (5-8). Progress has been made in identifying molecular markers for the i...

show abstract

Section: Discussionmentioning

confidence: 99%

NRAGE promotes the malignant phenotype of hepatocellular carcinoma

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Therefore, carbonaceous binder phase becomes the weakest part of the TiB 2 /C composite cathode, which begins the development bottleneck of composite cathodes. [7,8] The following major reasons were reported: (1) numerous pores emerge in composite cathodes after they are baked because of the low coking capacity of current commercial coal tar pitch, which may deteriorate the mechanical strength and conductivity of composite cathodes. Meanwhile, they penetrate electrolyte and sodium during aluminum electrolysis.…”

Section: Introductionmentioning

confidence: 98%

Thermal-Treated Pitches as Binders for TiB2/C Composite Cathodes

Lü

et al. 2011

Metall Mater Trans A

View full text Add to dashboard Cite

The effect of thermal treatment on the microstructure and properties of pitches and thermaltreated, pitch-based TiB 2 /C composite cathodes were investigated. Thermal treatments were performed at 473 K, 523 K, 573 K, 623 K, and 673 K (200°C, 250°C, 300°C, 350°C, and 400°C), respectively. The results show that the aromaticity of the treated pitches increases with an increasing thermal treatment temperature, and subsequently, the coking value and quinolineinsoluble (QI) content increase from 60.62 wt pct to 79.09 wt. pct and from 8.97 wt pct to 32.54 wt pct when the treatment temperature increases from 473 K to 623 K (200°C to 350°C). The volume fraction of coalesced mesophase in semicoke decreases with an increasing thermal treatment temperature, and after 673 K (400°C) is reached, the coalesced mesophase is almost invisible. The bulk density and compressive strength of modified pitch-based cathodes increase with an increasing thermal treatment temperature from 2.24 g cm À3 to 2.39 g cm À3 and from 24.21 MPa to 54.85 MPa, whereas open porosity decreases from 34.62 pct to 27.06 pct. Both electrical resistivity and electrolysis expansion ratio first decrease and then increase with an increasing thermal treatment temperature, and the lowest values (45.63 lX m and 0.65 pct) are achieved at 573 K (300°C). Compared with those of the parent pitch-based cathode, the properties of the modified pitch-based cathodes had improved significantly. The mechanisms of the improvements are discussed in the text.

show abstract

“…Initially, NRAGE was reported as a cancer suppressor gene, which promotes cell apoptosis via binding to P75NTR [13], Che-1 [14], XIAP-TAK1-TAB1 [15], and UNC5H1 [16] and inhibits proliferation [17] and angiogenesis [18]. Contradictorily, functions such as pro-apoptotic gene and growth promotion were slowly discovered [9,11,[19][20][21][22]. Kodera et al [21] found that increased NRAGE expression affects the malignant phenotype of HCC via its interaction with AATF.…”

Section: Introductionmentioning

confidence: 99%

“…Originally, our previous studies indicated that NRAGE was signi cantly overexpressed in the nucleus of ESCC cells with radioresistance [19] and knockdown NRAGE has signi cantly enhanced radiosensitivity in established radioresistant ESCC cells [11]. Scantily, there were only instantaneous intervention at constructed radioresistant EC cells cultured in the 2D level and no correlation with OS of ESCC.…”

Section: Introductionmentioning

confidence: 99%

NRAGE, a potential biomarker, induces radioresistance in two- and three-dimensional culture and confers poor prognosis in esophageal squamous cell carcinoma

Hong-bin¹,

Wang²,

Xiao³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background To explore the mechanism of NRAGE enhancing radioresistance of ESCC in 2D and 3D levels. Methods Stably NRAGE-overexpressed ESCC cells and 3D-printing models for ESCC cells were established. Then, cellular malignancy indexes, such as cell morphology, proliferation, radioresistance, motility, apoptosis, cell cycle, and proteins of the Wnt/β-catenin pathway, were compared between radioresistant and its parental cells in 2D and 3D levels. Additionally, 44 paraffin ESCC specimens with radical radiotherapy were selected to examine NRAGE and β-catenin protein expression and analyze the clinical correlation. Results Experiments in 2D culture showed that Eca109/NRAGE cells’ morphology was more irregular, elongated spindle-shaped and disappeared polarity. It obtained faster growth ability, stronger resistance to irradiation, enhanced motility, reduced apoptosis ratio and cell cycle rearrangement. Moreover, Western blot results showed β-catenin, p-Gsk-3β and CyclinD1 expressions were induced, while p-β-catenin and Gsk-3β expressions decreased in Eca109/NRAGE cells. Experiments in the 3D-printing model showed Eca109/NRAGE cell-laden 3D scaffolds had the advantage on growth and spheroiding according to the brigbtfield observation, scanning electron microscopy and Ki-67 IHC staining, and higher expression at the β-catenin protein. Clinical analysis showed that NRAGE expression was higher in tumor tissues than in control tissues of ESCC patients from the Public DataBase. Compared with radiotherapy effective group, both NRAGE total and nuclear and β-catenin nuclear expressions were significantly upregulated from ESCC specimens in invalid group. Further analysis showed a positive and linear correlation between NRAGE nuclear and β-catenin nuclear expressions. Additionally, results from univariate and multivariate analyses revealed NRAGE nuclear expression could serve as a risk factor for ESCC patients receiving radical radiotherapy. Conclusion ESCC cells with NRAGE nuclear accumulation demonstrated greater radioresistance, which may be related to the activation of the Wnt/β-catenin signaling pathway. It indicated that NRAGE nuclear expression was a potential biomarker for monitoring radiotherapeutic response.

show abstract

Relationship between NRAGE and the radioresistance of esophageal carcinoma cell line TE13R120

Cited by 14 publications

References 7 publications

NRAGE promotes the malignant phenotype of hepatocellular carcinoma

NRAGE promotes the malignant phenotype of hepatocellular carcinoma

Thermal-Treated Pitches as Binders for TiB2/C Composite Cathodes

NRAGE, a potential biomarker, induces radioresistance in two- and three-dimensional culture and confers poor prognosis in esophageal squamous cell carcinoma

Contact Info

Product

Resources

About