Relationship between oxidative stress levels and activation state on a hepatic stellate cell line

Abstract

The differences in physiological functions of stellate cell phenotypes suggest a relationship between oxidative stress levels and activation state.

Activity and expression of ecto-5′-nucleotidase/CD73 are increased during phenotype conversion of a hepatic stellate cell line

“…The experimental model of the permanent cell line, GRX, is known to be converted to quiescent-like HSC cells when treated with indomethacin or retinol for 7 days, decreasing proliferation and extracellular matrix production, accumulating neutral lipids and retinyl esters in perinuclear lipids droplet, reducing activation markers, modifying the extracellular matrix production, reorganizing the cytoskeleton and reprogramming the metabolism (Margis and Borojevic, 1989;Margis et al, 1992;Vicente et al, 1997Vicente et al, , 1998Guma et al, 2001;Mermelstein et al, 2001;Andrade et al, 2003;Guimaraes et al, 2006).…”

“…All results were analyzed by the 2 − ΔΔCT method (Livak and Schmittgen, 2001). β-actin was used as the internal control gene for all relative expression calculations (Guimaraes et al, 2006). The real-time PCR products were separated by 2.0% agarose gel electrophoresis and visualized by ethidium bromide staining.…”

Regulation of LPS stimulated ROS production in peritoneal macrophages from alloxan-induced diabetic rats: Involvement of high glucose and PPARγ

“…Reactive oxygen species were measured as previously described (Guimaraes et al, 2006) with minor modifications. Briefly, peritoneal or RAW 264.7 macrophages in 24-well plates were incubated with the treatments and in the last 30 min of treatment 2′,7′-dichlorofluorescein diacetate (DCFH-DA) (10 μM) was added and incubated in the dark at 37°C.…”

Antioxidant and anti-inflammatory action of melatonin in an experimental model of secondary biliary cirrhosis induced by bile duct ligation

“…Studies have shown that HSCs are directly involved in the process of fibrosis formation and that their activation is influenced by products generated from lipid peroxidation (LPO), formation of reactive oxygen species (ROS), and presence of inflammatory mediators such as tumor necrosis factor-alpha (TNF-α), inducible nitric oxide synthase (iNOS), interleukins, and nuclear factor-kappa B[4,6]. …”