Relationship Between Phenolic Content Determined by LC/MS/MS and Antioxidant Capacity and Enzyme Inhibition of Cyclotrichium niveum L.

Abstract

Cyclotrichium niveum (Boiss.) Manden & Scheng belonging to the Lamiaceae family, which is an endemic species in the eastern Anatolian region of Turkey, has an important place in terms of ethno-botany. The phytochemical composition of the plant, inhibition of acetylcholinesterase (AChE) (which hydrolyzes the neurotransmitter acetylcholine), inhibition of paraoxonase for antiatherosclerotic activity (hPON 1) (which detoxifies organophosphates), and antioxidant capacity were all investigated in this study. Phytochemical content was determined by LC/MS/MS, and enzyme inhibition and antioxidant capacity studies were determined by spectrophotometer. Antioxidant capacity of C. niveum extracts (methanol, hexane, and water) was determined by applying ABTS * + , DPPH * , FRAP, and CUPRAC methods. Both the water and the methanol extracts of the C. niveum exhibited significant inhibition on the AChE (IC 50 value for methanol and water extract 0.114 � 0.14 mg/ mL (R2:0.997) and 0.178 � 0.12 mg/mL (R 2 : 0.994), respectively). In contrast, the methanol and water extracts of the C. niveum did not exhibit the inhibition effect on hPON 1. The highest activity for ABTS * + was 66.53 % in the water extract, and DPPH * was 55.03 % in the methanol extract. In the metal-reducing power assay, the absorbance was 0.168 � 0.04 for FRAP water extract and 0.621 � 0.01 for CUPRAC methanol extract. According to LC/MS/MS analyses, hydroxybenzoic acid, salicylic acid, syringic acid, acetohydroxamic acid and luteolin determined in the plant extract. As a consequence, C. niveum which has antioxidant, anti-atherogenic and antineurodegenerative properties has the potential to be used as a natural medication instead of synthetic drugs used in Alzheimer's patients.

Telephi̇um İmperati̇'ni̇n Lc-MS/MS İle Poli̇fenolleri̇n Taranmasi Ve Anti̇oksi̇dan Özelli̇kleri̇

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Phytochemical profiling and In-vitro antioxidant activity of In-vivo α- amylase and α- glucosidase inhibitory activities of kidney Costus spicatus extract in diabetic Albino wistar Rats

“…The solution was kept in the dark for 1 hour, and the absorbance was measured at 390 nm. H2O2 concentrations were calculated using a standard curve and following established methods (15,42).…”

Phytochemical Analysis and Antioxidant, Anticholinesterase and Antiatherogenic Activity of Hairy Tea (Stachys lavandulifolia)

“…Asetilkolinesteraz inhibitör aktivitelerini değerlendirmek için Ellman'ın (Ellman ve ark., 1961). Ayrıca paraoksonaz inhibitör aktivitelerini göstermek için bir önceki çalışmamızdaki protokol kullanılmıştır (Güzel, 2023).…”