Relationship between postsynaptic NK₁ receptor distribution and nerve terminals innervating myenteric neurons in the guinea‐pig ileum

Abstract

The amounts of neurokinin 1 (NK 1 ) receptor immunolabelling on the membranes of myenteric cell bodies at appositions with tachykinin-immunoreactive nerve terminals, other nerve terminals, and glial cells were compared at the ultrastructural level using pre-embedding, double-label immunocytochemistry. NK 1 receptor immunoreactivity was revealed using silver-intensified, 1 nm gold, and tachykinin-immunoreactive nerve terminals were revealed using diaminobenzidine. The density of NK 1 receptor immunolabelling (silver particles per length of cell membrane) on the membrane at appositions with tachykinin-immunoreactive nerve terminals was not significantly different from that at appositions with other (nonimmunoreactive) nerve terminals or with glial cells. Synaptic specializations ("active zones") were present at a small proportion of the appositions between NK 1 receptor-immunoreactive cell bodies and tachykinin-immunoreactive or other nerve terminals. The density of NK 1 receptor immunolabelling at synaptic specializations was lower than that at regions of appositions where no synaptic specializations were present. The presence of NK 1 receptor on the cell surface in areas not directly apposed to tachykinin-containing nerve terminals suggests that tachykinins that diffuse away from their site of release may still exert an action via NK 1 receptors. Although NK 1 receptors do not appear to be targetted to particular sites on the surfaces of myenteric nerve cell bodies and proximal dendrites, they are reduced in density at regions of the membrane-forming synaptic specializations. Anat

Identification of neurons that express 5-hydroxytryptamine4 receptors in intestine

“…We interpret the staining to be at the surface membranes of the neurons, although this localization can only be established by electron-microscopic analysis. In a previous study involving the electron-microscopic examination of NK1 receptor localization, the receptor was almost continuously present along the surface membrane, whereas presynaptic terminals were irregularly located around the neurons (Portbury et al 2001). Earlier electron-microscopic studies have also reported that only parts of the surfaces of enteric neurons are contacted by presynaptic terminals (Gabella 1972;Komuro et al 1982).…”