Relationship between serum HBsAg level, HBV DNA level, and peripheral immune cells in patients with chronic hepatitis B virus infection

Mukherjee, RM; Reddy, P. Balkumar; Arava, Jyothi; Rao, PN; Sasikala, Mitnala; Gupta, Rajesh; Reddy, D. Nageshwar

doi:10.2147/hmer.s13796

Cited by 16 publications

(16 citation statements)

References 19 publications

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“…This was similar to results of studies carried in Cameroon by Tufon et al [16] and can be attributed to cell mediated liver cell damage and not cytopathic effect of the virus [22]. From this study mean values of AST (106.7U/L) and (60.7 U/L) were higher in both HBV positive and negative subjects compared to ALT (76.96 U/L) and (37.61 U/L) respectively.…”

Section: Discussionsupporting

confidence: 92%

Incidence of Elevated Aminotransferases Levels among Patients Presenting with Hepatitis Syndrome in the Northwest Region of Cameroon

Abongwa¹

2018

CJMI

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Hepatitis B (HB) and Hepatitis C (HC) are a leading cause of liver enzyme elevations in patients (LEE). This study aimed to estimate the prevalence of LEE among patients with hepatitis syndrome. This study enrolled 192 participants presenting with signs and symptoms of hepatitis in the Northwest Region of Cameroon from August 2015 to May 2016. Venous blood samples were collected for the diagnosis of HBV and HCV. Enzymatic measurement of ALT and AST was done using a biochemical analyzer. ALT and AST were categorised using standardized toxicity grade on upper limit of normal.The age ranged of the patients was 2-98years with a mean(SD) age of 41.42(±18.04). Of the 192 patients 97(50.5%) were female, HBV and HCV prevalences were 100(52.1%) and 0(0.0%) respectively. The prevalences of HBV were insignificantly (p=>0.05) higher in female 54(56.8%) and in the age range<30years 31(58.5%). ALT and AST activities showed significant (p <0.05) higher values 76.9±10.6 and 106.7±14.4, respectively in HBV positive than in HBV negative individuals. The prevalence of elevated ALT and AST enzymes were 68(35.4%) and 85(44.3%) respectively. Age and gender-wise prevalances showed high prevalence in >50years and in male. Elevated and significant transaminase activities were found in HBV positive compared to negative subjects. Alcoholic liver disease was common in HBV negative patients while chronic liver disease was higher among HBV positive patients (P =0.012). Elevated ALT and AST levels are common in both HBV positive and HBV negative subjects indicating multifactorial causes. As such effective management of hepatotoxicity requires accurate diagnosis. Materials and Methods Study design, site and populationThis study was carried out in the Northwest region of Cameroon from August 2015 to May 2016. It was a hospital based crosssectional study which enrolled participants who presented with signs and symptoms of hepatitis in the outpatient department of Mezam Polyclinic and Regional Hospitals. The exclusion criteria were participants who refused to be part of the study, patients who were known to be seropositive for HBV or HCV and those with known HIV positive status. Sample size was calculated based on Raosoft online calculator using the level of significance of 0.05 and power of 80% assuming that the expected prevalence is 10%.[17] A total of 192 patients of both sexes exceeded the minimum required sample size of 97. Ethical considerationEthical clearance was obtained from the institution ethical review boards. Clients above 18 years and guardians of children 18 years and below were verbally consented. The aims of the study and procedure to be taken were explained to them and their confidentiality ensured prior to sample collection. Laboratory procedureAfter venous blood collection, samples were allowed to clot and centrifuged at 3000 rmp for 3mins. HBV and HCV were diagnosed using HBV and HCV Diaspot strips (Jakarta, Indonesia) which detect HBsAg or HCV respectively from the patient serum. ALT and AST were measure enzymatic...

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Section: Discussionsupporting

confidence: 92%

Incidence of Elevated Aminotransferases Levels among Patients Presenting with Hepatitis Syndrome in the Northwest Region of Cameroon

Abongwa¹

2018

CJMI

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“…Keeping in mind the reported findings of immune stimulatory effects of HBV towards other cells of the immune system, such as Kupffer cells in liver (Hosel, 2009), the significant drop in the mRNA expression of both RANTES and IP-10 as observed in our group of cirrhotic subjects might have insinuations in promoting lesser recruitment of immunocompetent cells from peripheral pool to liver to avert further damage. This conjecture can be conjured up to our earlier finding of significant suppression of CD8 T cells in CHB subjects without any numerical increment in the T helper (CD4) or NK cell populations (Mukherjee, 2010). Of note , Crawford et al (2011) reported a significant reduction in CD8 T cell numbers and function in the absence of RANTES in chronic viral infection while the CD4 T cells remain unaffected in such situation.…”

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confidence: 56%

“…For expression of data, HBsAg concentrations obtained in ng/mL were converted to IU/mL, considering that 0.15 ng/mL of HBsAg is equivalent to 350 IU/ml as mentioned before (Biswas, 2003;Mukherjee, 2010).…”

Section: Quantitation Of Serum Hbsagmentioning

confidence: 99%

Reduced Expression of Human Chemokine Genes RANTES and IP-10 in Hepatitis B Virus Mediated Cirrhosis of Liver

Shravanti¹,

Mukherjee²,

Rao³

et al. 2012

JBLS

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Regulated upon Activation Normal T cell Expressed and Secreted (RANTES) and interferon gamma inducible protein 10 (IP-10), both chemokines are chemotactic for immunocompetent cells and play an important role in cell mediated antiviral defense. The objective of this work was to assess the expression pattern of RANTES and IP-10 genes in peripheral blood mononuclear cells (PBMCs) of hepatitis B virus (HBV) infected patients having various ISSN 2157-6076 2012 www.macrothink.org/jbls 221 disease severity. The study was performed on 79 HBV infected patients grouped into acute, inactive carriers (IC), chronic (CHB), cirrhosis and hepatocellular carcinoma (HCC) plus 41 healthy voluntary blood donors as controls. Quantification of HBV surface antigen (HBsAg) was done by a sandwich enzyme linked immunosorbent assay (ELISA). Conventional and real time polymerase chain reaction (PCR) were used for genotyping and determination of HBV DNA load respectively. RANTES and IP-10 mRNA expressions were evaluated by reverse transcription PCR (RT-PCR) and densitometry. Results obtained show that RANTES expression reduced significantly (p<0.0001) in cirrhosis group in comparison to controls and remain unaltered in other disease categories. Reduction in IP-10 expression was significant (p=0.006) in patients of all disease categories than controls which was most evident in cirrhosis group (p<0.0001). No association was found between the expression level of chemokines with HBV genotypes, HBsAg and HBV DNA levels in sera. Journal of Biology and Life ScienceIt could be concluded that reduced expression of both the chemokines might be associated with lesser infiltration of immunocompetent cells to liver to avert further damage in cirrhosis.Serum level of both RANTES and IP-10 can be considered as prognostic marker of liver cirrhosis by validation studies.

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“…The liver injury in hepatitis B is the result of cell mediated hepatic injury rather than cyopathic effect of the virus. 16 A higher percentage (85.66%) of patients showed normal AST level that could be explained by mild injury of liver hepatocytes. 17 Generally, ALT and AST are recognized markers for liver diseases.…”

Section: Discussionmentioning

confidence: 96%

Characteristics of patients with HBeAg negative chronic hepatitis B virus infection in Duhok, Kurdistan region

Merza¹,

Yousif

Younus

et al. 2018

Int J Res Med Sci

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Background: The objective of this study was to characterize patients with HBeAg-negative chronic hepatitis B in Duhok province.Methods: We recruited all patients with HBeAg negative chronic hepatitis B virus infections who visited viral hepatitis clinic in Azadi Teaching Hospital between September 2015-December 2017. The main evaluation parameters were: serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels, serum albumin, total serum bilirubin (TSB), viral load. Few patients were subjected to Fibro-test, Fibroscan, or liver biopsy.Results: There were 251 patients. The mean age of the patients was 35.75±14.40 years. One hundred fifty-seven cases were male and 94 cases were female. The baseline mean of ALT, AST and viral load was 42.09±2.71U/L, 30.26±19.65U/L, and 1421197.08±14436692.04IU/ml, respectively. Compared with women, men have significantly higher values of ALT and AST, serum albumin, and TSB. There were 217 chronic inactive carrier (IC) patients and 34 chronic active hepatitis (AH) patients. ALT, AST, and viral load levels were significantly higher among AH patients.Conclusions: The majority of patients showed nil to mild liver injury. The higher level of ALT in males is a supporting guide to follow the American Association for the Study of Liver Diseases (AASLD) considering 30U/L and 19U/L levels for males and females, respectively. There was preponderance of males in AH, which indicates greater frequency of advanced fibrosis in such patients. There was a greater probability of AH in old aged people. Serum albumin and TSB were not good markers for differentiation between AH and IC state. Twenty-four patients had ALT above twice upper limit of normal level, but 34 patients showed AH based on liver biopsy, firboscan or fibrotest.

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Relationship between serum HBsAg level, HBV DNA level, and peripheral immune cells in patients with chronic hepatitis B virus infection

Cited by 16 publications

References 19 publications

Incidence of Elevated Aminotransferases Levels among Patients Presenting with Hepatitis Syndrome in the Northwest Region of Cameroon

Incidence of Elevated Aminotransferases Levels among Patients Presenting with Hepatitis Syndrome in the Northwest Region of Cameroon

Reduced Expression of Human Chemokine Genes RANTES and IP-10 in Hepatitis B Virus Mediated Cirrhosis of Liver

Characteristics of patients with HBeAg negative chronic hepatitis B virus infection in Duhok, Kurdistan region

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