Relationship between sperm motility characteristics and ATP concentrations, and association with fertility in two different pig breeds

Tremoen, Nina Hårdnes; Gaustad, Ann Helen; Andersen-Ranberg, Ina; Son, Maren van; Zeremichael, Teklu Tewoldebrhan; Frydenlund, Kirsten; Grindflek, Eli; Våge, Dag Inge; Myromslien, Frøydis Deinboll

doi:10.1016/j.anireprosci.2018.04.075

Cited by 32 publications

(39 citation statements)

References 29 publications

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“…Algunos trabajos han hecho aportes en relación con las subpoblaciones y la capacidad fecundante de los eyaculados (Holt et al, 1996;Abaigar et al, 1999;Vyt et al, 2008). En cuanto a la fertilidad, diversos autores han demostrado que existe alguna relación entre los parámetros de cinética espermática y la fertilidad (Holt et al, 1997;Broekhuijse et al, 2012;Schulze et al, 2013;Yániz et al, 2017;Tremoen et al, 2018;Winters et al, 2018).…”

Section: Discussionunclassified

Frecuencia de fotogramas óptima para evaluar la cinética espermática de verracos con un sistema CASA-Mot

et al. 2021

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Introducción. La movilidad es el indicador de calidad seminal más utilizado, por tanto, es necesario determinar una adecuada frecuencia de fotogramas (frame rate, FR) para estandarizar los análisis cinéticos con los equipos de análisis computadorizado de semen (CASA-Mot) y disminuir la variabilidad entre laboratorios, lo que optimizará el número de dosis seminales y su calidad para la industria porcina basada en inseminación artificial (IA). Objetivo. Determinar la frecuencia óptima de fotogramas sobre la cinética espermática de verraco mediante un sistema comercial CASA-Mot y analizar la distribución de subpoblaciones espermáticas con el FR óptimo. Materiales y métodos. Se utilizaron veinte dosis seminales de diez verracos Pietrain (Sus scrofa domestica). El periodo experimental fue entre febrero y julio del año 2017. Se utilizó el sistema CASA-Mot (ISAS®v1) con cámaras de recuento ISAS®D4C20 precalentadas a 37 °C. El tiempo de captura fue de dos segundos. Resultados. La frecuencia de fotogramas afectó los parámetros de cinética espermática, exceptuando la velocidad rectilínea (VSL), debido a que no representó un comportamiento dependiente del FR. Las demás variables presentaron alteraciones significativas al cambiar su FR, y la velocidad curvilínea (VCL) y velocidad promedio (VAP) fueron las variables más sensibles al cambio. Se obtuvieron tres componentes principales (PC) definidos por patrones de movilidad, progresividad y oscilación, en donde el PC1 (velocidad-ondulación) fue el más influyente, debido a que representó el 51,34 % y 55,08 % de la varianza total explicada para 25 y 200 fps (fotogramas por segundo), respectivamente. Conclusión. La frecuencia óptima de fotogramas para analizar variables de cinética espermática en verraco con un sistema CASA-Mot fue de 200 fps. Una tasa de fotogramas inferior provoca errores en la estimación de la trayectoria de los espermatozoides. La distribución de subpoblaciones espermáticas varía según la tasa de fotogramas utilizada. Es necesario establecer un protocolo de análisis para homogenizar los resultados.

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Section: Discussionunclassified

Frecuencia de fotogramas óptima para evaluar la cinética espermática de verracos con un sistema CASA-Mot

et al. 2021

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“…In terms of artificial insemination, the linearity (LIN) of sperm movement on the day of collection and the wobble (WOB) after storage influenced the total number of piglets born (TNB) in Norwegian Landrace, while the percentage of motile cells, curvilinear velocity (VCL) and lateral head amplitude (ALH) on the day of semen collection and LIN after storage influenced TNB in Norwegian Duroc (Tremoen et al, 2018). Concurrently, the variables VCL, ALH, LIN and WOB affected TNB when using one or more assessment models, and all these variables among the threshold values (receiver operating characteristic (ROC) curve analysis) defining the swimming pattern of hyperactive sperm (Schmidt and Kamp, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…It manifests an active mechanism and membrane integrity and is positively correlated with fertilising capacity (Johnson et al, 2000;Vyt et al, 2004). During recent years, computer-aided sperm analysis (CASA) systems have been recommended by the revised WHO manual (World Health Organization, 2010) as a selective means to analyse semen samples, and have been widely used to assess sperm motility characteristics in boar semen (Vyt et al, 2004;Purdy et al, 2010;Tretipskul et al, 2010;Tremoen et al, 2018;Pearodwong et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Determination of the optimal concentration of Minitube Equex paste for boar semen cryopreservation based on sperm motility characteristics

2020

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Equex paste is a non-permeating cryoprotective agent (CPA) that improved post-thaw survival of spermatozoa during boar semen cryopreservation. However, Equex paste produced by Nova Chemical Sales Inc. (MA, USA) is not currently available. The aim of the present study was to determine the optimal concentration of Minitube Equex paste (Minitube, Tiefenbach, Germany) for boar semen cryopreservation in comparison with Nova Equex STM paste (control). Fifteen ejaculates from 12 mature boars were collected by the glove-hand method. Each ejaculate was aliquoted and cryopreserved in base freezing extender III as Tris-citrate egg yolk (TEY) extender plus 9.0% glycerol classified into four groups. Group I was the control and included only 1.5% Nova Equex STM paste. Groups II, III and IV were the experiment groups, and contained different concentrations of Minitube Equex paste (Group II: 1.5%; Group III: 1.7%; and Group IV: 1.9%) added to the freezing extender III. After freezing and thawing, sperm motility characteristics were evaluated by Sperm Class Analyzer® incubated at 37 °C for 0 (10 min), 1 and 2 h post-thawing. In Group IV after thawing at 0 h, rapid velocity and the velocity curved line were significantly higher than in Groups II and III (P < 0.05) but did not differ from Group I. Moreover, after thawing at 1 h, LIN (linearity) in Group IV was higher than in Group II (P < 0.05), but did not differ from the other groups. In conclusion, the most suitable concentration of Minitube Equex paste in the current protocol was 1.9% supplemented with 9.0% glycerol in TEY-based freezing extender III, based on the conformity between data from manual guides and the observed sperm motility characteristics results.

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“…Movement of the sperm cells requires constant energy supplementation (e.g., ATP) provided by functional mitochondria. Defective or dysfunctional sperm mitochondria has been shown to associate with low fertility in many species, including humans, horses, and dogs [ 8 , 33 , 34 , 35 , 36 ]. We detected severe decline in sperm membrane potential after cryopreservation process, suggesting that the function of mitochondria was affected after cryopreservation.…”

Section: Discussionmentioning

confidence: 99%

Novel Low-Voltage Electro-Ejaculation Approach for Sperm Collection from Zoo Captive Lanyu Miniature Pigs (Sus barbatus sumatranus)

Chen

Chang

et al. 2020

Animals

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Semen collection can be achieved via hand penile massage or rectal stimulation using electro-ejaculation methods. Traditional electro-ejaculation procedure applied relatively high voltage of 3–15 volts with a maximum current of 900 mA. However, these manipulations often result in great stress and discomforts in animals. In this study, we showed low-voltage electro-ejaculation procedure using 2–3 volts with a maximum current of 500 mA can efficiently stimulated ejaculations in zoo captive lanyu miniature pigs with a high success rate of 81.3% (13/16). Besides normal semen properties (semen volume, pH, sperm concentration), we demonstrated that low-voltage electro-ejaculation caused less stress in the animals, and sperm cells obtained via low-voltage electro-ejaculation exhibit low abnormality (10.3%), high viability (84.3%), motility (75.7%), progressive motility (63.7%), and acrosome integrity (88%). However, cryopreservation protocol used in the current study requires further optimization, as sperm mitochondrial function was partially compromised during freezing procedures. Taken together, we demonstrated in this study that a low-voltage electro-ejaculation approach can be used to obtain quality sperm cells from zoo captive lanyu miniature pig with less physical stress during electro-ejaculation procedure.

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Relationship between sperm motility characteristics and ATP concentrations, and association with fertility in two different pig breeds

Cited by 32 publications

References 29 publications

Frecuencia de fotogramas óptima para evaluar la cinética espermática de verracos con un sistema CASA-Mot

Frecuencia de fotogramas óptima para evaluar la cinética espermática de verracos con un sistema CASA-Mot

Determination of the optimal concentration of Minitube Equex paste for boar semen cryopreservation based on sperm motility characteristics

Novel Low-Voltage Electro-Ejaculation Approach for Sperm Collection from Zoo Captive Lanyu Miniature Pigs (Sus barbatus sumatranus)

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