Relationship between Superoxide dismutase (SOD) and viral liver diseases

Inagaki, T.; K, Katoh; Takiya, Satoshi; Ikuta, Koji; Kobayashi, Susumu; Suzuki, Masahiro; Fukuzawa, Yoshitaka; Ayakawa, Tadao; Shimizu, Kazuhito; Tagaya, Tuneaki; Katoh, K.

doi:10.1007/bf02777758

Cited by 15 publications

(13 citation statements)

References 10 publications

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“…The characteristics of the immunopositive distribution of Mn-SOD in HCC tissues in that report 15 appear to be essentially the same as our findings, especially in noncancerous tissue adjacent to tumors. The rate of immunopositivity for Mn-SOD in HCC tissues in our study, however, was higher than that reported by inagaki et al 15 The reasons for this discrepancy appear to be either differences in the technical procedure for immunohistochemistry or differences in the specificty of the antibody for Mn-SOD. Since we used the monoclonal antibody raised against human liver Mn-SOD recognizing the COOH terminal peptide, 9 the most likely explanation for the discrepancy would appear to be differences in antibody specificity.…”

Section: Discussioncontrasting

confidence: 84%

Immunohistochemical expression of manganese superoxide dismutase in hepatocellular carcinoma, using a specific monoclonal antibody

Maeyama

Takakuwa

et al. 1994

J Gastroenterol

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The expression of manganese superoxide dismutase (Mn-SOD) was studied immunohistochemically, using a specific monoclonal antibody, in surgically resected hepatocellular carcinoma (HCC) and noncancerous tissues from 47 patients (2 with well-differentiated HCC, 36 with moderately differentiated HCC, 8 with poorly differentiated HCC, and 1 with undifferentiated carcinoma). Cancer cells in 44 patients (93.6%) were positive for Mn-SOD. The staining pattern of cancer cells was mostly homogeneous in well-differentiated HCC, whereas it was heterogeneous in poorly differentiated HCC. Moreover, strongly positive immunoreactivity was observed in noncancerous liver tissues in all patients, especially in normal hepatocytes surrounding HCC, regenerative small hepatocytes in the tumor boundary, and mononuclear inflammatory cells in the necroinflammatory lesions. The positive immunoreactivity for Mn-SOD in patients with HCC appears to reflect increased production of the enzyme protein.

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Section: Discussioncontrasting

confidence: 84%

Immunohistochemical expression of manganese superoxide dismutase in hepatocellular carcinoma, using a specific monoclonal antibody

Maeyama

Takakuwa

et al. 1994

J Gastroenterol

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“…Several clinical studies have also showed a causal relationship between HCC and low levels of super-oxide dismutase (SOD) [5][6][7][8]. In our investigation of the long-term effects of CuZn superoxide dismutase (CuZn-SOD) deficiency in mutant animals, we observed the development of HCC in B6-Sod1 (gene designation for CuZn-SOD)−/− mice by 21 months of age [9].…”

Section: Introductionmentioning

confidence: 77%

Identification of biomarkers associated with the development of hepatocellular carcinoma in CuZn superoxide dismutase deficient mice

et al. 2007

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To identify biomarkers associated with the development of hepatocellular carcinoma (HCC) in CuZn superoxide dismutase (CuZnSOD, Sod1) deficient mice, 2-DE followed by MS analysis was carried out with liver samples obtained from 18-month-old Sod1−/− and +/+ mice. The intra-cellular Ca binding protein, regucalcin (RGN), showed a divergent alteration in Sod1−/− samples. Whereas elevated RGN levels were observed in −/− samples with no obvious neoplastic changes, marked reduction in RGN was observed in −/− samples with fully developed HCC. GST mu1 (GSTM1), on the other hand, showed a significant increase only in the neoplastic regions obtained from Sod1−/− livers. No change in GSTM1 was observed in the surrounding normal tissues. Marked reduction was observed in two intracellular lipid transporters, fatty acid binding protein 1 (FABP1) and major urinary protein 11 and 8 (MUP 11&8), in Sod1−/− samples. Analysis of additional samples at 18−22 months of age showed a three-fold increase in enolase activities in Sod1−/− livers. Consistent with previous findings, carbonic anhydrase 3 (CAIII) levels were significantly reduced in Sod1−/− samples, and immunohistochemical analysis revealed that the reduction was not homogenous throughout the lobular structure in the liver.

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“…Immunohistochemical studies of SOD activity revealed that hepatocarcinogenesis induced by viral infection is closely related to the depletion of SOD in liver tissues (23). Further, HCC mediated by hepatitis C virus infection is associated with increased lipid peroxidation (24).…”

Section: Discussionmentioning

confidence: 99%

Effects of Benzo(a)pyrene on the Expression of Heat Shock Proteins, Pro-inflammatory Cytokines and Antioxidant Enzymes in Hepatic Tumors Induced by Rat Hepatoma N1-S1 Cells

et al. 2011

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Benzo(a)pyrene (BaP) is a polycyclic aromatic hydrocarbon (PAH) that is easily introduced to humans via consumption of grilled or smoked meat. BaP causes harmful oxidative effects on cell development, growth and survival through an increase in membrane lipid peroxidation, oxidative DNA damage and mutagenesis. Therefore, the present study was conducted to evaluate the synergistic effects of BaP on oxidative stress in hepatic tumors. In this study, we established a hepatic tumor model by injecting rat hepatoma N1-S1 cells into healthy rats. Changes in the abundance of heat shock proteins (HSPs), antioxidant enzymes and pro-inflammatory cytokines were then investigated by western blot analysis. In addition, we examined changes in oxidative stress levels. Injection of N1-S1 cells or concomitant injection of BaP and N1-S1 cells resulted in the formation of hepatic tumors at the injection site. Evaluation of rat plasma reveals that hepatic tumors induced by BaP and N1-S1 cells expresses higher levels of Hsp27, superoxide dismutase (SOD), and tumor necrosis factor-α (TNF-α) when compared to those induced by N1-S1 cells only. The collective results of this study suggest that BaP exerts synergistic effects on the expression of HSP, cytokines and antioxidant enzymes in hepatic tumors induced by rat hepatoma N1-S1 cells.

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Relationship between Superoxide dismutase (SOD) and viral liver diseases

Cited by 15 publications

References 10 publications

Immunohistochemical expression of manganese superoxide dismutase in hepatocellular carcinoma, using a specific monoclonal antibody

Immunohistochemical expression of manganese superoxide dismutase in hepatocellular carcinoma, using a specific monoclonal antibody

Identification of biomarkers associated with the development of hepatocellular carcinoma in CuZn superoxide dismutase deficient mice

Effects of Benzo(a)pyrene on the Expression of Heat Shock Proteins, Pro-inflammatory Cytokines and Antioxidant Enzymes in Hepatic Tumors Induced by Rat Hepatoma N1-S1 Cells

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