Relationship between Symptoms and Gene Expression Induced by the Infection of Three Strains of Rice dwarf virus

Satoh, Kouji; Shimizu, Takumi; Kondoh, Hiroaki; Hiraguri, Akihiro; Sasaya, Takahide; Choi, Il-Ryong; Omura, Toshihiro; Kikuchi, Shoshi

doi:10.1371/journal.pone.0018094

Cited by 49 publications

(54 citation statements)

References 70 publications

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“…Also, we find induction of a set of defense related genes including PR genes, WRKY transcription factors and several genes functioning in RNA silencing. This is consistent with reports of Shimizu et al [9] and Satoh et al [7] showing that increased expression of defense related genes may be a common response of rice infected with RDV [7,40]. However, our results indicate that RDV induced the expression of far more genes than it suppressed.…”

Section: Discussionsupporting

confidence: 93%

Transcriptome profiling confirmed correlations between symptoms and transcriptional changes in RDV infected rice and revealed nucleolus as a possible target of RDV manipulation

Yang

Gao

et al. 2014

Virol J

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BackgroundRice dwarf virus (RDV) is the causal agent of rice dwarf disease, which limits rice production in many areas of south East Asia. Transcriptional changes of rice in response to RDV infection have been characterized by Shimizu et al. and Satoh et al.. Both studies found induction of defense related genes and correlations between transcriptional changes and symptom development in RDV-infected rice. However, the same rice cultivar, namely Nipponbare belonging to the Japonic subspecies of rice was used in both studies.MethodsGene expression changes of the indica subspecies of rice, namely Oryza sativa L. ssp. indica cv Yixiang2292 that show moderate resistance to RDV, in response to RDV infection were characterized using an Affymetrix Rice Genome Array. Differentially expressed genes (DEGs) were classified according to their Gene Ontology (GO) annotation. The effects of transient expression of Pns11 in Nicotiana benthaminana on the expression of nucleolar genes were studied using real-time PCR (RT-PCR).Results856 genes involved in defense or other physiological processes were identified to be DEGs, most of which showed up-regulation. Ribosome- and nucleolus related genes were significantly enriched in the DEGs. Representative genes related to nucleolar function exhibited altered expression in N. benthaminana plants transiently expressing Pns11 of RDV.ConclusionsInduction of defense related genes is common for rice infected with RDV. There is a co-relation between symptom severity and transcriptional alteration in RDV infected rice. Besides ribosome, RDV may also target nucleolus to manipulate the translation machinery of rice. Given the tight links between nucleolus and ribosome, it is intriguing to speculate that RDV may enhance expression of ribosomal genes by targeting nucleolus through Pns11.

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Section: Discussionsupporting

confidence: 93%

Transcriptome profiling confirmed correlations between symptoms and transcriptional changes in RDV infected rice and revealed nucleolus as a possible target of RDV manipulation

Yang

Gao

et al. 2014

Virol J

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“…1 Data from Satoh et al (2013) .2 Unpublished data by K. Satoh .3 Data from Satoh et al (2010) .4 Data with RDV strain S from Satoh et al (2011) .5 Change in expression not significant .6 Expression not detected .…”

Section: Resultsmentioning

confidence: 99%

Suppression of cell wall-related genes associated with stunting of Oryza glaberrima infected with Rice tungro spherical virus

et al. 2014

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Rice tungro disease is a complex disease caused by the interaction between Rice tungro bacilliform virus and Rice tungro spherical virus (RTSV). RTSV alone does not cause recognizable symptoms in most Asian rice (Oryza sativa) plants, whereas some African rice (O. glaberrima) plants were found to become stunted by RTSV. Stunting of rice plants by virus infections usually accompanies the suppression of various cell wall-related genes. The expression of cell wall-related genes was examined in O. glaberrima and O. sativa infected with RTSV to see the relationship between the severity of stunting and the suppression of cell wall-related genes by RTSV. The heights of four accessions of O. glaberrima were found to decline by 14–34% at 28 days post-inoculation (dpi) with RTSV, whereas the height reduction of O. sativa plants by RTSV was not significant. RTSV accumulated more in O. glaberrima plants than in O. sativa plants, but the level of RTSV accumulation was not correlated with the degree of height reduction among the four accessions of O. glaberrima. Examination for expression of genes for cellulose synthase A5 (CESA5) and A6 (CESA6), cellulose synthase-like A9 (CSLA9) and C7, and α-expansin 1 (expansin 1) and 15 precursors in O. glaberrima and O. sativa plants between 7 and 28 dpi with RTSV showed that the genes such as those for CESA5, CESA6, CSLA9, and expansin 1were more significantly suppressed in stunted plants of O. glaberrima at 14 dpi with RTSV than in O. sativa, suggesting that stunting of O. glaberrima might be associated with these cell wall-related genes suppressed by RTSV. Examination for expression of these genes in O. sativa plants infected with other rice viruses in previous studies indicated that the suppression of the expansin 1 gene is likely to be a signature response commonly associated with virus-induced stunting of Oryza species. These results suggest that stunting of O. glaberrima by RTSV infection might be associated with the suppression of these cell wall-related genes at the early stage of infection with RTSV.

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“…Microarray profiling after biotic treatments [rice stripe virus (RSV) and rice tungro spherical virus (RTSV)] in rice seedlings has revealed six OsNAC genes induced by both virus infections (Nuruzzaman et al, 2010). Rice plants with a mutation in rim1-1 are resistant to infection by dwarf virus (Yoshii et al, 2009; Saga et al, 2011). The StNAC ( Solanum tuberosum ) gene is induced in response to Phytophthora infestans infection (Collinge and Boller, 2001).…”

Section: Introductionmentioning

confidence: 99%

Roles of NAC transcription factors in the regulation of biotic and abiotic stress responses in plants

Nuruzzaman¹,

Sharoni²,

Kikuchi³

2013

Front. Microbiol.

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435

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NAC transcription factors are one of the largest families of transcriptional regulators in plants, and members of the NAC gene family have been suggested to play important roles in the regulation of the transcriptional reprogramming associated with plant stress responses. A phylogenetic analysis of NAC genes, with a focus on rice and Arabidopsis, was performed. Herein, we present an overview of the regulation of the stress responsive NAC SNAC/(IX) group of genes that are implicated in the resistance to different stresses. SNAC factors have important roles for the control of biotic and abiotic stresses tolerance and that their overexpression can improve stress tolerance via biotechnological approaches. We also review the recent progress in elucidating the roles of NAC transcription factors in plant biotic and abiotic stresses. Modification of the expression pattern of transcription factor genes and/or changes in their activity contribute to the elaboration of various signaling pathways and regulatory networks. However, a single NAC gene often responds to several stress factors, and their protein products may participate in the regulation of several seemingly disparate processes as negative or positive regulators. Additionally, the NAC proteins function via auto-regulation or cross-regulation is extensively found among NAC genes. These observations assist in the understanding of the complex mechanisms of signaling and transcriptional reprogramming controlled by NAC proteins.

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Relationship between Symptoms and Gene Expression Induced by the Infection of Three Strains of Rice dwarf virus

Cited by 49 publications

References 70 publications

Transcriptome profiling confirmed correlations between symptoms and transcriptional changes in RDV infected rice and revealed nucleolus as a possible target of RDV manipulation

Transcriptome profiling confirmed correlations between symptoms and transcriptional changes in RDV infected rice and revealed nucleolus as a possible target of RDV manipulation

Suppression of cell wall-related genes associated with stunting of Oryza glaberrima infected with Rice tungro spherical virus

Roles of NAC transcription factors in the regulation of biotic and abiotic stress responses in plants

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