Relationship between the Corynebacterium pseudotuberculosis , phospholipase D inoculation and the fertility characteristics of crossbred Boer bucks

Abstract

The aim of this study was to investigate the effects of Corynebacterium pseudotuberculosis and phospholipase D (PLD) on buck's fertility, specifically, testosterone concentration, scrotal circumference and semen quality. Crossbred Boer goats (n=13) aged 12-14 months were divided into three groups. The first group (n=3) was inoculated with one ml sterile phosphate buffer saline subcutaneously into subaxillary area as the control. The second group (n=5) was inoculated with live C. pseudotuberculosis 1×10 9 cfu subcutaneously into subaxillary area. The third group (n=5) was inoculated with PLD 1 ml/20 kg BW intravenously into jugular vein. Blood collection was done twice a week over a period of three months for testosterone analyses. Semen was collected using electro-ejaculator once every two weeks whilst scrotal circumference was measured once every three weeks. Semen volume, semen pH and sperm progressive motility, concentration, live/dead percentage and morphology were evaluated. Bucks were euthanized three months' post inoculation and histopathological examination of the testicles and epididymis was performed. The results showed seven folds' significant decrease (P<0.05) in testosterone concentration in both C. pseudotuberculosis and PLD inoculated groups compared to the control. Semen volume, percentage of dead/live and abnormal sperm morphology showed significant increase (P<0.05) in both inoculated groups compared to the control. The following sperm defects were observed; tapered sperm, decapitated and knobbed head, thickened midpiece, distal and proximal droplet, sterilizing tail and folded tail. Scrotal circumference, semen pH, semen wave pattern, sperm motility and concentration showed significant decrease (P<0.05) in both inoculated groups compared to the control. The testicles showed varied degrees of degeneration and necrosis with shrunken seminferous tubules. In conclusion, testosterone concentration, scrotal circumference and semen quality were negatively affected in both inoculated groups. Moreover, percentages of live/dead and abnormal sperm morphology were also increased. The results suggest that both C. pseudotuberculosis and PLD had detrimental effects on buck's fertility.

Hematological effects for rabbits immunized by Corynebacterium pseudotuberculosis sonicated antigen

“…The four mentioned factors could influence the levels of blood cells that circulate at the blood vessels, which affect total and differential white blood cells count as what was found by [23,24]. A report [25] assumed that the CLA at its initial stage might facilitate multiplication of C. pseudotuberculosis within the macrophages themselves [8], However, phospholipase-D might have a positive influence in infections through playing part in escaping of germs from hydrolysis process inside the macrophages, effecting by that at the inner layer of the phospholipid for the macrophage's cell membrane [26] for the previous reason we found our results are logically accepted for their variation between infected and immunized groups.…”

Immune Responses of Goats to Corynebacterium pseudotuberculosis and its Mycolic Acids (MAs) Extract

“…C aseous lymphadenitis (CLA) is a chronic infectious disease that has an affinity for sheep and goats (Baird & Fontaine, 2007;Abdullah et al, 2019). The causative agent C. pseudotuberculosis is an intracellular gram-positive bacteria (Batey, 1986;Mahmood et al, 2016). The infection (CLA) occurs in two distinct forms; the external and internal forms (Lopez et al, 1966;Jesse et al, 2017).…”

Utility assessment of an Enzyme‐linked immunosorbent assay for detection of subclinical cases of caseous lymphadenitis in small ruminant flocks

“…This mechanism cause plasma flow from inside the vessels to lymph nodes and for increased vascular permeability. PLD is resposible for many biologic actions such as dermo necrosis, destruction of macrophages in goat's infections, interferes with the process of chemotaxis of neutrophils in sheep and is lethal to phagocytic cells, actions that contributes to the dissemination of the agent from the point of infection to other parts of the organism (Aquino de Sá et al, 2013;Smith Bradford, 2015;Mahmood et al, 2016 The disease occurs worldwide with high prevalence values and causes serious economic losses, particularly in sheep farms and it is more evident in small producers (Al-Gaabary, Osman, Ahmed, & Oreiby, 2010;Aquino de Sá et al, 2013;Arsenault et al, 2003;Jung et al, 2015;Malone et al, 2006;Zavoshti, Khoojine, Helan, Hassanzadeh, & Heydari, 2012). CLA is considered one of the most important diseases, in economic terms, of sheep and goats in the USA, Canada and Brazil causing significant losses in countries where the disease is considered endemic (Çetinkaya et al, 2002;Farias et al, 2019;Guimarães, 2011;Latif et al, 2016;Paton, 2003;Pavan, Robles, Cairó, Marcelino, & Pettinari, 2011;Windsor, 2014).…”