Relationship of Bactericidal Permeability Increasing Protein (BPI) Polymorphysm rs1341023, rs5743507, Tumor Necrosis Factor Alpha (TNF-α) rs361525, rs1800629 with Neonatal Sepsis

Abstract

Background: Neonatal sepsis is a problem in the field of child health because the incidence is increasing every year and often ends in death. This study aimed to investigate the relationship between TNF-α and BPI gene polymorphisms with neonatal sepsis.
 Methods: PCR and sequencing examinations were performed on 60 DNA samples consisting of 30 samples of neonatal sepsis and 30 samples of non-neoptic sepsis. Furthermore, data in the form of DNA mutation tables were statistically processed by univariate, bivariate, and multivariate analysis. It is considered meaningful if p<0.05.
 Result: The results showed that BPI rs1341023, rs5743507 and TNF-α rs361525, rs1800629 only BPI rs1341023 and TNF-α rs1800629 were mutated. Of the two genes, only TNF-α rs1800629 had a significant association with neonatal sepsis (p<0.05).
 Conclusion: This study proved that the TNF-α rs1800629 mutation was the most important polymorphism in the occurrence of neonatal sepsis (p<0.05).
 Keywords: BPI, TNF-α, Neonatal, Polymorphism, Sepsis

A meta-analysis of the association between inflammatory cytokine polymorphism and neonatal sepsis

“…We initially searched 1129 articles, of which 202 were in Chinese and 927 were in English. After a series of screening, 29 articles were finally included in the meta-analysis, including 4 articles [32][33][34][35] related to IL-1, 9 articles [34,[36][37][38][39][40][41][42][43] to IL-6, 2 articles [36,44] related to IL-8, 6 articles [38,43,[44][45][46][47] related to IL-10, and 8 articles [34,36,41,[48][49][50][51][52] related to TNF-α (see Figs 1-5). Basic information, genotype distribution, and quality of the included studies (NOS scores � 5 for all included studies) are shown in Table 1.…”

“…Tatjana Varljen et al [8] found that TNF-α-308A allele may be a risk factor for earlyonset neonatal sepsis, while Lakshmi Srinivasan et al [28] did not find an association between tumor necrosis factor-308g /A polymorphism and sepsis. Therefore, we summarized relevant literature at home and abroad [34,36,41,[48][49][50][51][52] and found that the OR value of TNF-α-308G/A (rs1800629) polymorphism merged in the allele model was statistically significant (P = 0.016), indicating a significant increase in the risk of neonatal sepsis with the A allele (OR = 1.257, 95% CI: 1.044-1.513). The OR value merged in the recessive gene model was statistically significant (P = 0.009), indicating a significant increase in the risk of sepsis in newborns with the AA genotype (OR = 1.913, 95% CI: 1.179-3.104).…”