Relationships between cold hardiness and diapause, and between glycerol and free amino acid contents in overwintering larvae of the oriental corn borer, Ostrinia furnacalis

Goto, Masahiko; Sekine, Yutaka; Outa, Hitoshi; Hujikura, Mikio; Suzuki, Koichi

doi:10.1016/s0022-1910(00)00099-8

Cited by 62 publications

(48 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[37][38][39] The correlation between increased concentrations of alanine and cold tolerance has been reported for a large number of insect species. 9,22 In support of this, the colligative effectiveness of alanine is roughly the same as that of glycerol. 19 Moreover, alanine is a less toxic end product of pyruvate catabolism than lactate, which may be important during diapause when the aerobic portion of cellular respiration is slowed.…”

Section: Resultsmentioning

confidence: 67%

“…[8][9][10] Several eco-physiological and biochemical studies have been conducted to improve our understanding of the cellular mechanisms of cold hardening process during the hypometabolic state. 11,12 The main function of metabolism during periods of cold accli-Pura} et al : Metabolomic Analysis of Diapausing and Noni-diapausing ... mation is biosynthesis of low molecular weight organic solutes such as polyols and sugars that depress the body's supercooling point (cryoprotectants), while stabilizing proteins and cellular membranes.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Metabolomic Analysis of Diapausing and Noni-diapausing Larvae of the European Corn Borer Ostrinia nubilalis (Hbn.) (Lepidoptera: Crambidae)

Purać

Kojić

Popović

et al. 2015

ACSi

View full text Add to dashboard Cite

In this study, an 1 H-NMR -based metabolomic approach was used to investigate the biochemical mechanisms of diapause and cold hardiness in diapausing larvae of the European corn borer Ostrinia nubilalis. Metabolomic patterns in polar hemolymph extracts from non-diapausing and diapausing larvae of O. nubilalis were compared. Analysis indicated 13 metabolites: 7 amino acids, glycerol, acetate, citrate, succinate, lactate and putrescine. Results show that diapausing larvae display different metabolomic patterns compared to active non-diapausing larvae, with predominant metabolites identified as glycerol, proline and alanine. In specific diapausing larvae initially kept at 5 °C then gradually chilled to -3 °C and -16 °C, alanine , glycerol and acetate were predominant metabolites. 1 H-NMR spectroscopy provides new insight into the metabolomic patterns associated with cold resistance and diapause in O. nubilalis larvae, suggesting distinct metabolomes function in actively developing and diapausing larvae.

show abstract

Section: Resultsmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Metabolomic Analysis of Diapausing and Noni-diapausing Larvae of the European Corn Borer Ostrinia nubilalis (Hbn.) (Lepidoptera: Crambidae)

Purać

Kojić

Popović

et al. 2015

ACSi

View full text Add to dashboard Cite

show abstract

“…The numbers of live and dead larvae were recorded. Dead larvae were determined as those with no movement and exhibiting loose body segments (Goto et al, 2001). Recorded larvae were not reused, and larvae that died during acclimation were not included in the -14°C exposure test.…”

Section: Exposure Experiments At -14°cmentioning

confidence: 99%

The effect of temperature on the diapause and cold hardiness of Dendrolimus tabulaeformis (Lepidoptera: Lasiocampidae)

Zeng¹,

Ge²,

Su³

et al. 2008

Eur. J. Entomol.

View full text Add to dashboard Cite

Abstract. Pine caterpillar, Dendrolimus tabulaeformis Tsai et Liu, is a major pine pest in North China. The larvae enter diapause in the third or fourth instar before winter. Supercooling points (SCP) and cold hardiness of the diapausing larvae were investigated and compared in non-acclimated, acclimated and de-acclimated larvae. A bimodal frequency distribution was observed with a break point of -14°C in the SCP. Larvae in the low group (LG, SCP -14°C) were more cold tolerant with lower lethal temperatures than those in the high group (HG, SCP > -14°C). This bimodality occurred in three patterns, LG (> 60% of individuals in LG), LG-HG (< 60% of individuals in LG and HG) and HG (> 60% of individuals in HG), in response to cold acclimation and de-acclimation. The cold hardiness was ranked as: LG > LG-HG > HG pattern. Cold hardiness was enhanced by an increase in concentrations of trehalose, galactose, glucose and mannose in the haemolymph as well as by decrease in metabolism after cold acclimation, but was lost after de-acclimation. Loss of cold hardiness was correlated with decrease in sugars and increase in metabolic rate. In conclusion, the species is a chill tolerant insect, adopting the strategy of depressing SCP through accumulation of low molecular weight sugars in the haemolymph, concomitant with metabolic depression.

show abstract

“…In vitro experiments have demonstrated that alanine acts as a cryoprotectant, maintaining enzyme activities at low temperatures Carpenter et al, 1990), and several studies have observed an alanine upregulation in insects during cold acclimation (Fields et al, 1998;Goto et Table 1. Denlinger, 2007).…”

Section: Discussionmentioning

confidence: 99%

Do current environmental conditions explain physiological and metabolic responses of subterranean crustaceans to cold?

Colson-Proch

Renault

Gravot

et al. 2009

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARY Subterranean environments are characterized by the quasi absence of thermal variations (±1°C within a year), and organisms living in these biotopes for several millions of years, such as hypogean crustaceans, can be expected to have adapted to this very stable habitat. As hypogean organisms experience minimal thermal variation in their native biotopes, they should not be able to develop any particular cold adaptations to cope with thermal fluctuations. Indeed, physiological responses of organisms to an environmental stress are proportional to the amplitude of the stress they endure in their habitats. Surprisingly, previous studies have shown that a population of an aquatic hypogean crustacean, Niphargus rhenorhodanensis, exhibited a high level of cold hardiness. Subterranean environments thus appeared not to be following the classical above-mentioned theory. To confirm this counter-example, we studied seven karstic populations of N. rhenorhodanensis living in aquifers at approximately 10°C all year round and we analysed their behavioural, metabolic and biochemical responses during cold exposure (3°C). These seven populations showed reduced activities, and some cryoprotective molecules were accumulated. More surprisingly, the amplitude of the response varied greatly among the seven populations, despite their exposure to similar thermal conditions. Thus, the overall relationship that can be established between the amplitude of thermal variations and cold-hardiness abilities of ectotherm species may be more complex in subterranean crustaceans than in other arthropods.

show abstract

Relationships between cold hardiness and diapause, and between glycerol and free amino acid contents in overwintering larvae of the oriental corn borer, Ostrinia furnacalis

Cited by 62 publications

References 21 publications

Metabolomic Analysis of Diapausing and Noni-diapausing Larvae of the European Corn Borer Ostrinia nubilalis (Hbn.) (Lepidoptera: Crambidae)

Metabolomic Analysis of Diapausing and Noni-diapausing Larvae of the European Corn Borer Ostrinia nubilalis (Hbn.) (Lepidoptera: Crambidae)

The effect of temperature on the diapause and cold hardiness of Dendrolimus tabulaeformis (Lepidoptera: Lasiocampidae)

Do current environmental conditions explain physiological and metabolic responses of subterranean crustaceans to cold?

Contact Info

Product

Resources

About