Relationships between denitrification gene expression, dissimilatory nitrate reduction to ammonium and nitrous oxide and dinitrogen production in montane grassland soils

Chen, Zhe; Wang, Changhui; Gschwendtner, Silvia; Willibald, Georg; Unteregelsbacher, Sebastian; Lu, Haiyan; Kolar, Allison; Schloter, Michael; Butterbach‐Bahl, Klaus; Dannenmann, Michael

doi:10.1016/j.soilbio.2015.03.030

Cited by 62 publications

(48 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Chen et al. () found that changing climatic conditions decreased the abundance of norB genes (DNA), but promoted the expression of norB (RNA). In order to confirm the hypothesized increases in denitrifier activity, future work should focus on the expression of denitrifying genes through RNA‐based analyses.…”

Section: Discussionmentioning

confidence: 99%

Intermittent flooding of organic‐rich soil promotes the formation of denitrification hot moments and hot spots

et al. 2019

View full text Add to dashboard Cite

Intermittent flooding of organic-rich soil promotes the formation of denitrification hot moments and hot spots.Abstract. Anthropogenic activities have altered the nitrogen cycle, necessitating management on the landscape level. Isolated time periods and areas, termed hot moments and hot spots, respectively, frequently account for a large percentage of nitrate removal in aquatic ecosystems. A series of experiments were conducted to determine the effect of hydrologic connectivity on denitrification rates, gene abundances, and nitrous oxide fluxes. Experimental areas were divided into flooded (always inundated), floodzone (intermittently inundated), and nonfloodzone (not inundated) locations in low-organic and organic-rich soil. Our results demonstrate that intermittent flood events enhance denitrification rates from days to weeks after flooding, depending on the inundation period. Microbial analysis demonstrated that short-term flood events did not lead to increases in denitrifying gene abundances. Enhanced denitrification rates did not have a corresponding increase in the ratio of incomplete to complete denitrification. Incomplete to complete denitrification ratios were high in always-inundated low-organic sandy soil, peaking at 40%. Our results suggest that management strategies that promote hydrologic connectivity and intermittent flooding of organic-rich floodplain soils promote the formation of denitrification hot moments and hot spots, with relatively low incomplete denitrification (<3% of the total denitrification rates).

show abstract

Section: Discussionmentioning

confidence: 99%

Intermittent flooding of organic‐rich soil promotes the formation of denitrification hot moments and hot spots

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The soil air of the four soil cores and the headspace of the incubation vessel were completely replaced by a gas mixture consisting of 20% O 2 (purity grade of 5.5), 80% He (purity grade of 5.0), N 2 O (400 ppbv) and N 2 (25 ppmv). This complete exchange was done by automated repeated cycles of evacuation and gas purging, achieved through a built-in purging system in an extremely air-tight chamber that is connected directly to a gas chromatograph (Shimadzu GC-17A, Shimadzu, Munich, Germany)17262728. Eighteen hours of evacuation-purging cycles ensure a complete removal of the background atmospheric air27, after which the headspace and tubing connections to the gas chromatograph were further purged for three hours.…”

Section: Methodsmentioning

confidence: 99%

“…Based on the sensitivity and repeatability of the gas chromatograph measurements, the detection limits were <0.03 ppmv h −1 for N 2 and <0.45 ppbv h −1 for N 2 O. The measured N 2 flux from the soil equals to gross N 2 O consumption whereas the sum of N 2 and N 2 O fluxes equals to gross N 2 O production17262728.…”

Section: Methodsmentioning

confidence: 99%

Disentangling gross N2O production and consumption in soil

Wen¹,

Chen

Dannenmann

et al. 2016

Sci Rep

Self Cite

View full text Add to dashboard Cite

The difficulty of measuring gross N2O production and consumption in soil impedes our ability to predict N2O dynamics across the soil-atmosphere interface. Our study aimed to disentangle these processes by comparing measurements from gas-flow soil core (GFSC) and 15N2O pool dilution (15N2OPD) methods. GFSC directly measures soil N2O and N2 fluxes, with their sum as the gross N2O production, whereas 15N2OPD involves addition of 15N2O into a chamber headspace and measuring its isotopic dilution over time. Measurements were conducted on intact soil cores from grassland, cropland, beech and pine forests. Across sites, gross N2O production and consumption measured by 15N2OPD were only 10% and 6%, respectively, of those measured by GFSC. However, 15N2OPD remains the only method that can be used under field conditions to measure atmospheric N2O uptake in soil. We propose to use different terminologies for the gross N2O fluxes that these two methods quantified. For 15N2OPD, we suggest using ‘gross N2O emission and uptake’, which encompass gas exchange within the 15N2O-labelled, soil air-filled pores. For GFSC, ‘gross N2O production and consumption’ can be used, which includes both N2O emitted into the soil air-filled pores and N2O directly consumed, forming N2, in soil anaerobic microsites.

show abstract

“…DNRA are less understood than denitrification although it can be a significant or even a dominant process in some ecosystems, [25]. Conditions favouring DNRA may include high temperature [26] and high C/N ratio, [27]. [28].…”

Section: Eqmentioning

confidence: 99%

Subsurface nitrate reduction under wetlands takes place in narrow superficial zones

Ribas

Calderer

Martí

et al. 2017

Environmental Technology

View full text Add to dashboard Cite

show abstract

Relationships between denitrification gene expression, dissimilatory nitrate reduction to ammonium and nitrous oxide and dinitrogen production in montane grassland soils

Cited by 62 publications

References 56 publications

Intermittent flooding of organic‐rich soil promotes the formation of denitrification hot moments and hot spots

Intermittent flooding of organic‐rich soil promotes the formation of denitrification hot moments and hot spots

Disentangling gross N2O production and consumption in soil

Subsurface nitrate reduction under wetlands takes place in narrow superficial zones

Contact Info

Product

Resources

About