Relationships between <i>Xanthomonas campestris</i> pv. <i>vitians</i> population sizes, stomatal density and lettuce resistance to bacterial leaf spot

Nicolas, Olbert; Charles, Marie Thérèse; Jenni, Sylvie; Toussaint, Vicky; Beaulieu, Carole

doi:10.1080/07060661.2018.1495269

Cited by 10 publications

(12 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fourteen days post inoculation, symptoms were observed on the lettuce leaves according to their degree of resistance to BLS as described in Nicolas et al (2018).…”

Section: Resultsmentioning

confidence: 99%

“…The lettuce cultivars used in this study were previously characterized for their resistance to BLS by Nicolas et al (2018; Table 1), according to a modified version of the method developed by Bull et al (2015). Cultivars were classified into three groups based on their susceptibility to BLS: tolerant group with Batavia Reine des Glaces (BRG), Little Gem (LIG), Estival (EST) and Hochelaga (HOC); intermediate group with Romora (ROM) and Turbo (TUR); and susceptible group with Chief (CHI), Gorilla (GOR), Paris Island Cos (PIC) and Vista Verde (VIV).…”

Section: Methodsmentioning

confidence: 99%

“…Cultivars were classified into three groups based on their susceptibility to BLS: tolerant group with Batavia Reine des Glaces (BRG), Little Gem (LIG), Estival (EST) and Hochelaga (HOC); intermediate group with Romora (ROM) and Turbo (TUR); and susceptible group with Chief (CHI), Gorilla (GOR), Paris Island Cos (PIC) and Vista Verde (VIV). These cultivars are representative lettuce types from the Agriculture and Agri-Food Canada lettuce breeding program (Nicolas et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

“…Different levels of resistance to BLS have been reported in lettuce, and the single dominant Xanthomonas resistance gene 1 ( Xar1 ) has been identified as being responsible for resistance in some lettuce cultivars (Hayes et al, 2014). Other factors such as stomatal density of the leaves have been also reported to play a role in lettuce resistance (Nicolas et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Ionomics of Lettuce Infected by Xanthomonas campestris pv. vitians

et al. 2019

Self Cite

View full text Add to dashboard Cite

Bacterial leaf spot (BLS) caused by Xanthomonas campestris pv. vitians ( Xcv ) places a major constraint on lettuce production worldwide. The most sustainable strategy known to date for controlling BLS is the use of resistant cultivars. The nutrient elemental signature (ionome) of ten lettuce cultivars with three levels of resistance was analyzed by inductively coupled plasma optical emission spectroscopy (ICP-OES) to determine which nutrient balances are linked to resistance to BLS, and to assess the effect of Xcv infection on the ionome. The elemental concentrations were preprocessed with isometric log-ratios to define nutrient balances. Using this approach, 4 out of 11 univariate nutrient balances were found to significantly influence the resistance of lettuce cultivars to BLS ( P < 0.05). These significant balances were the overall nutritional status balancing all measured nutrients with their complementary in the dry mass, as well as balances [Mn | Zn,Cu], [Zn | Cu], and [S,N | P]. Moreover, the infection of lettuce cultivars mostly affected the lettuce ionome on the [N,S | P] balance, where infection tended to lean the balance toward the N,S part relatively to P. This study shows that nutrient uptake in lettuce can be affected by BLS infection and that nutrient status influences resistance to BLS infection.

show abstract

“…Fourteen days post inoculation, symptoms were observed on the lettuce leaves according to their degree of resistance to BLS as described in Nicolas et al (2018).…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Ionomics of Lettuce Infected by Xanthomonas campestris pv. vitians

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…One of the most devastating diseases of lettuce is bacterial leaf spot (BLS) disease, caused by Xanthomonas campestris. Water soaked lesions appear on the margins of the leaves, reducing the quality and affecting the market value of lettuce (Nicolas et al, 2018). In Quebec (Canada), severe outbreaks of BLS have led to devastating economic losses (i.e., 100% of the lettuce in the field is ruined).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Biosurfactant-Producing Bacteria From Oil Well Batteries With Antimicrobial Activities Against Food-Borne and Plant Pathogens

2020

View full text Add to dashboard Cite

Microbial biosurfactants, produced by fungi, yeast, and bacteria, are surface-active compounds with emulsifying properties that have a number of known activities, including the solubilization of microbial biofilms. In an ongoing survey to uncover new or enhanced antimicrobial metabolite-producing microbes from harsh environments, such as oil-rich niches, 123 bacterial strains were isolated from three oil batteries in the region of Chauvin, Alberta, and characterized by 16S rRNA gene sequencing. Based on their nucleotide sequences, the strains are associated with 3 phyla (Actinobacteria, Proteobacteria and Firmicutes), as well as 17 other discrete genera that shared high homology with known sequences, with the majority of these strains identified to the species level. The most prevalent strains associated with the three oil wells belonged to the Bacillus genus. Thirty-four of the 123 strains were identified as biosurfactantproducers, among which Bacillus methylotrophicus strain OB9 exhibited the highest biosurfactant activity based on multiple screening methods and a comparative analysis with the commercially available biosurfactant, Tween 20. B. methylotrophicus OB9 was selected for further antimicrobial analysis and addition of live cultures of B. methylotrophicus OB9 (or partially purified biosurfactant fractions thereof) were highly effective on biofilm disruption in agar diffusion assays against several Gram-negative food-borne bacteria and plant pathogens. Upon co-culturing with B. methylotrophicus OB9, the number of either Salmonella enterica subsp. enterica Newport SL1 or Xanthomonas campestris B07.007 cells significantly decreased after 6 h and were not retrieved from co-cultures following 12 h exposure. These results also translated to studies on plants, where bacterized tomato seedlings with OB9 significantly protected the tomato leaves from Salmonella enterica Newport SL1 contamination, as evidenced by a 40% reduction of log 10 CFU of Salmonella/mg leaf tissue compared to nonbacterized tomato leaves. When B. methylotrophicus 0B9 was used for bacterized lettuce, the growth of X. campestris B07.007, the causal agent of bacterial leaf spot of lettuce, was completely inhibited. While limited, these studies are noteworthy as

show abstract