Relationships between immunoglobulin M and immunoglobulin G or A in colostrum of Japanese black multiparous cows

Abstract

This study was conducted to clarify the relationships among immunoglobulin (Ig)M, IgG, IgA, β-carotene, vitamin A and α-tocopherol contents in colostrum of 24 Japanese Black multiparous cows in order to evaluate the role of IgM on colostral IgG and IgA production. Compared with colostral IgG, colostral IgM and IgA were very low but varied widely. There was positive correlation between colostral IgM and IgG, but colostral IgM was not related with colostral IgA. There was no relationship between colostral IgM and age of cows, although colostral IgG was increased with aging. There were positive correlations among colostral β-carotene, vitamin A and α-tocopherol and these vitamins were positively correlated with colostral IgM and IgG. These results indicate that fat-soluble vitamins may affect colostral IgG and IgM in cows and colostral IgG increases with the increase of colostral IgM.

Effects of supplemental β‐carotene on colostral immunoglobulin and plasma β‐carotene and immunoglobulin in Japanese Black cows

“…Blood was sampled by a jugular vein puncture into ethylenediaminetetraacetic acid (EDTA)‐2Na vacuum tubes and then centrifuged at 1,670 × g for 10 min at 4°C. Samples of colostrum collected from the control ( n = 8) or β‐carotene ( n = 9) groups within 3 hr after parturition were used for Ig analyses, because colostral Ig concentrations in Japanese Black cows decrease rapidly 3 hr after parturition (Ishikawa, Serizawa, Ahiko, Asai, & Seike, ; Taniguchi et al., ). Samples of colostrum were taken from each udder of the cows by hand and 100 ml colostrum was stored at −20°C for chemical analyses.…”

“…The immunoassay of colostral and plasma IgG 1 , IgA and IgM in cows was determined as previously described (Nishiyama, Yasumatsuya, et al., ; Taniguchi et al., ). Colostral and plasma IgG 1 , IgA and IgM were measured using the Bovine IgG 1 , IgA and IgM ELISA Quantitation Kit (Bethyl Laboratories, Montgomery, AL, USA) and ELISA Starter Accessory Package (Bethyl Laboratories) according to the manufacturer's instructions.…”

“…Supplemental vitamin A and β‐carotene enhance the immune system in neonates (Chew & Park, ; Rühl, ), but β‐carotene‐deficient calves were found to have a higher incidence of diarrhea at 6 days of age (Kume & Toharmat, ). Colostral β‐carotene as well as colostral vitamin A were positively correlated with colostral IgG or IgM in Japanese Black cows (Taniguchi et al., ). Although supplemental β‐carotene or β‐carotene‐enriched dry carrots had no effects on colostral IgG, IgA and IgM concentrations in the cows fed high β‐carotene diets (Kaewlamun et al., ; Taniguchi et al., ), feeding β‐carotene‐enriched dry carrots improved colostral IgA and IgG 1 in β‐carotene‐deficient Japanese Black cows (Nishijima et al., ).…”

Fabrication of a poly(N‐vinyl‐2‐pyrrolidone) modified macroporous polypropylene membrane via one‐pot reversible‐addition fragmentation chain‐transfer polymerization and click chemistry

“…31 The flux ARTICLE WILEYONLINELIBRARY.COM/APP recovery improved over 50% for the PNVP-grafted poly(ether sulfone) membrane by the photoinduced grafting of PNVP. 32 The result in Figure 9 clearly shows that the BSA rejection increased with increasing GD; this was attributed to membrane pore shrinkage.…”

Insights into the surface property and blood compatibility of polyethersulfone/polyvinylpyrrolidone composite membranes: toward high-performance hemodialyzer

“…A characteristic morphology of asymmetric membrane consisting of a dense top layer and porous sublayer with a finger-like structure was observed for all the membranes. [45,46] For the PVP blended composite membranes (FSM-5-2 and FSM-5-7), the top skin layer and the finger-like structure were observed with a little change, porous sublayer was formed, and larger quantities of micropores in the finger-like structure were observed when PVP was added into PES matrix, especially the FSM-5-7. This should be attributed to the hydrophilic structure of PVP and the affinity between the PES chains and PVP; the PVP polymer would assembly with PES chains during the aqueous phase separation of the membrane fabrication.…”