Relationships between Lathyrus cassius, L. odoratus, and L. hirsutus Assessed by Experimental Hybridization, Analysis of Meiotic Pairing, and DNA: DNA Hybridization

In an attempt to introduce new variation into Lathyrus latifolius, the perennial pea, a programme of interspecific hybridization with several perennial and one annual Lathyrus species was undertaken. New interspecific hybrid combinations were produced involving three of the perennial species (L. latifolius, L. sylvestris, L. heterophyllus). Crosses with the annual species, L. gorgoni, were unsuccessful. These new hybrids are important additions to the limited number of Lathyrus hybrids. Meiotic analysis of the new hybrids and in particular the analysis of their pachytene pairing from spread synaptonemal complexes show that the low fertility of these hybrids is not due to structural differences between the chromosomes of the parental species. Some of the new hybrids that were produced showed unusual karyotypes and variation in meiotic chromosome pairing. (© Inra/Elsevier, Paris.) Lathyrus / chromosome analysis / interspecific hybridisation / synaptonemal complexes Résumé -Hybridations interspécifiques entre espèces pérennes du genre Lathyrus (Fabaceae). Dans le but d'introduire une nouvelle variabilité chez Lathyrus latifolius, le pois pérenne, un programme d'hybridation interspécifique a été entrepris entre espèces de Lathyrus, plusieurs pérennes et une annuelle. De nouvelles combinaisons hybrides interspécifiques ont été obtenues, impliquant trois des espèces pérennes (L. latifolius, L. sylvestris et L. heterophyllus). Les croisements avec l'espèce annuelle, L. gorgoni furent infructueux. Ces nouveaux hybrides constituent un apport important au nombre limité d'hybrides de Lathyrus actuellement disponibles. L'analyse des méioses des nouveaux hybrides et en particulier l'analyse de l'appariement au pachytène à partir d'étalements de complexes synaptonémaux montre que la faible fertilité de ces hybrides n'est pas due à des différences structurelles entre les chromosomes des espèces parentales. Quelques uns de ces nouveaux hybrides ont présenté des caryotypes atypiques et une variation dans l'appariement des chromosomes à la méiose. (© Inra/Elsevier, Paris.

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“…Murray and Hammett [13] and Murray et al [15]. Pollen viability was assessed using the method of Heslop-Harrison and Heslop-Harrison [9].…”

Section: Methodsmentioning

confidence: 99%

“…Despite the difficulty of making interspecific hybrids in Lathyrus, our group has been successful in producing a number of new combinations [6,13,15]. In general these new hybrid combinations have been more readily achieved between species that are taxonomically closely related and have similar karyotypes [5,6,13,21].…”

Section: Introductionmentioning

confidence: 99%

Interspecific hybridization in perennial species of Lathyrus (fabaceae)

Nandini

Hammett²,

Murray³

1999

Agronomie

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“…-=measurement not made. be hybridized experimentally (Murray & Hammett, 1989;Murray et al,, 1993;Hammett et al, 1994). These studies have also demonstrated significant chromosomal/genetical variation within accessions of both species.…”

Section: %mentioning

confidence: 99%

“…(Leguminosae) are difficult to make and there are many reports of extensive hybridization programmes that have resulted in remarkably few successes (Yamamoto et al, 1986;Murray & Hammett, 1989;Yunus &Jackson, 1991). As part of a wider programme aimed at the transfer of genes from wild Lathyrus species into the sweet pea (L. odoratus L.) we have produced a number of new hybrids (Murray & Hammett, 1989;Murray et al, 1993;Hammett et aZ., 1994). From these studies several interesting features have emerged.…”

Section: Introductionmentioning

confidence: 99%

New interspecific hybrids in Lathyrus (Leguminosae): Lathyrus annuus x L. hierosolymitanus

Hammett

Murray

Markham³

et al. 1996

Botanical Journal of the Linnean Society

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Experimental hybridization, cytogenetic observations, flower pigment analyses and scanning electron microscope observations of seed coat features were used to investigate the relationship between L. annuus L. and L. hieroso$rnitanus Boiss. Pod width, seed size, shape and surface ornamentation proved to be good diagnostic criteria for distinguishing the two taxa. Hybridization between various accessions of the two species revealed variation both between and within accessions. Enhanced anthocyanin levels were detected in the standard petals of the reciprocal hybrids made between two specific accessions of L. annuus and L. hierosolyrnifanus. The results of the investigations support the retention of L. annuus and L. hierosolymitanus as separate species.

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“…Morphological characters are most commonly used to assess relationships between species. Hybridization success and resultant progeny fertility have been widely used as reliable measures of closeness of parental species ( Murray et al ., 1993 ). In the genus Striga (Scrophulariaceae), some species form complex groups based on their morphological similarities ( Musselman et al ., 1991 ; Mohammed, 1994; Mohammed et al ., 1996 ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of variability in Striga aspera, Striga hermonthica and their hybrids using morphological characters and random amplified polymorphic DNA markers

et al. 2000

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Striga aspera and Striga hermonthica are recognized as separate species, but their close morphological similarity causes difficulty in distinguishing between them in areas where they coexist in Africa. In this study, crosses between the species were made using randomly selected morphologically typical parental plants collected from different locations in Nigeria. Genetic analysis of both species and their reciprocal F1 hybrids were determined using cluster analysis of DNA profiles derived from genetic polymorphism (RAPD)‐polymerase chain reaction (PCR) markers. Principal component and hierarchical cluster analyses were used to separate parental and hybrid populations based on 13 morphological characteristics. Morphological data from wild samples of both species were compared with the hand‐pollinated parental, F1 and F2 hybrids, and back‐crosses. Results showed that S. aspera and S. hermonthica were genetically and morphologically distinct. Morphological and genetic analyses revealed two major clusters: a S. aspera cluster and a S. hermonthica cluster. Genetically, the F1 hybrids showed closer affinity to their maternal parents, while morphologically, the F1 hybrids formed distinct clusters intermediate to the parents. Most F2 plants and back‐crosses were morphologically similar to S. hermonthica. Comparative morphological analysis of wild and hand‐pollinated populations showed some samples from the wild clustered with the hybrids, suggesting that hybrids may exist in nature.

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Relationships between Lathyrus cassius, L. odoratus, and L. hirsutus Assessed by Experimental Hybridization, Analysis of Meiotic Pairing, and DNA: DNA Hybridization

Cited by 7 publications

References 11 publications

Interspecific hybridization in perennial species of Lathyrus (fabaceae)

Interspecific hybridization in perennial species of Lathyrus (fabaceae)

New interspecific hybrids in Lathyrus (Leguminosae): Lathyrus annuus x L. hierosolymitanus

Evaluation of variability in Striga aspera, Striga hermonthica and their hybrids using morphological characters and random amplified polymorphic DNA markers

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