Relationships between temperate phages Mu and P1

Toussaint, Ariane; Lefebvre, N.; Scott, June R.; Cowan, Jack A.; Bruijn, F. de; Bukhari, Ahmad I.

doi:10.1016/0042-6822(78)90048-x

Cited by 57 publications

(29 citation statements)

References 47 publications

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“…MuG ( +) polypeptide (s) which are antigenically common to those of P1cinC( +) might differ in electrophoretic mobility or in size. When the analogy with the G segment of Mu was taken into account (Chow and Bukhari 1976;Toussaint et al 1978). it was expected that at least two sets of polypeptides could be detected in SDS-PAGE as the difference between the two P1cin mutants; in this experiment, difference in only one set of polypeptides, 105 and 101 kd, was detected.…”

Section: Discussionmentioning

confidence: 99%

“…The neutralization tests suggest that P1cinC(+) and PlcinC( -) bear the anti genically distinct polypeptide(s) as component(s) of the tail structure involved in host specificity though they still have common antigen (s) concerned with inf ectivity. MuG ( +) was partially neutralized with anti-P1cinC ( +) serum as reported previously (Toussaint et al 1978), but not with anti-P1cinC(-) serum, suggesting that the C ahd G segments might express infectious speci- Table 2. Characters of Plcin lysogens 9 ficity similar to each other only when they are in the same orientation.…”

Section: Neutralizationmentioning

confidence: 92%

“…MuG(+) was also tested since it has been reported (Toussaint et al 1978) that anti-P1 and anti-Mu sera partially neutralize Mu and P1, respectively.…”

Section: Neutralizationmentioning

confidence: 99%

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Isolation of P1cinC(+) and P1cinC(-) mutants and detection of their polypeptides involved in host specificity.

Tominaga

Nakamura

Enomoto

1986

The Japanese journal of genetics

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P1cinC (+) mutants were isolated from a P1 CMclr100 lysogen based on the criterion that the cmn mutation cannot restore flagellar phase variation of the hin pin strain, and a P1cinC (-) mutant was isolated from a P1cinC (+ ) lysogen of the hint pint strain by utilizing the difference in colonial types at 41°C. The hint pint/PlcinC (-) strain yielded P1cinC (+) lysogens at a rate of 10-2 per bacterium while the hin pin/PlcinC (-) strain was considerably stable. Anti-P1cinC (+) serum neutralized both the P1 cinC (-) and MuG (+ ) particles to 16% the rate of the homologous combination whereas antiP1cinC (-) serum neutralized the P1cinC (+) particles to 45% the rate of the homologous combination. This indicates that P1cinC(+) and P1cinC(-) bear antigenically distinct polypeptides concerned with host specificity and that P1cinC (+) shares some antigen (s) involved in inf ectivity with MuG (+ ). The analysis of whole phage proteins revealed that P1cinC(+) had a 105 kd polypeptide, and P1cinC(-) had 101 kd instead of 105 kd.

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Section: Discussionmentioning

confidence: 99%

Section: Neutralizationmentioning

confidence: 92%

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Isolation of P1cinC(+) and P1cinC(-) mutants and detection of their polypeptides involved in host specificity.

Tominaga

Nakamura

Enomoto

1986

The Japanese journal of genetics

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“…The gene organization of the P1CmS67-1 1 segment flanked by two inverted IS1 sequences. The region between ISIa and the middle of the C segment is non-essential for P1 vegetative reproduction as well as for P1 prophage propagation, whereas insertions between the middle of the C segment and ISld affect P1 phage growth (Arber et al., 1978, 1980Sengstag & Arber, 1983;Iida., 1984). The non-essential region includes the site-specific recombinase gene cin (C inversion) (Hiestancl-Nauer & , the lyd gene (lysis delayed) and the anti-restriction gene dur (defence against reslriction) (S. Iida, M. Streiff, T. Bickle and W. Arber, unpublished, cited in Yarmolinsky, 1982).…”

Section: Resultsmentioning

confidence: 99%

“…P1 lyd phages give very small plaques due to delayed lysis (Iida & Arber, 1977). The region that includes the right half of the invertible C segment cont,ains the gene(s) for the tail fibre which determines P1 host range (Toussaint et al, 1978;Walker & Walker, 1983;Iida, 1984). Genes for the base plate and possibly for the tail tube are mapped in the area between the C segment and ISId (Walker & Walker, 1983), while no information is available on the genomic organization of these late genes.…”

Section: Resultsmentioning

confidence: 99%

Bacteriophage P1 Derivatives Unaffected in Their Growth by a Large Inversion or by IS Insertions at Various Locations

Iida¹,

Meyer²,

Arber³

1985

Microbiology

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129Several plaque-forming phage P1 derivatives carrying DNA rearrangements associated with IS elements are described. They have ISl, IS3 and IS5 inserted in four distinct locations, all of which are non-essential regions for phage P1 propagation. One derivative carries a genome segment, inverted relative to the one in the P1 wild-type genome, between two inverted copies of ISl. The inverted DNA segment spans about 23 kb of the 90 kb long P1 genome and it includes the invertible C segment. This phage is as viable as an isomeric P1 which carries the relevant segment in its original orientation. These results are discussed with regard to the genome organization of phage P1.

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A site-specific, conservative recombination system carried by bacteriophage P1. Mapping the recombinase gene cin and the cross-over sites cix for the inversion of the C segment.

Iida¹,

Meyer²,

Kennedy³

et al. 1982

The EMBO Journal

102

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The bacteriophage P1 genome carries an invertible C segment consisting of 3‐kb unique sequences flanked by 0.6‐kb inverted repeats. With insertion and deletion mutants of P1 derivatives the site‐specific recombinase gene cin for C inversion) has been mapped adjacent to the C segment and the cix sites (for C inversion cross‐over) have been located at the outside ends of the inverted repeats. Inversion of the C segment functions as a biological switch and controls expression of the gene(s) responsible for phage infectivity carried on the C segment. The cin gene product can promote recombination between a ‘quasi‐ cix’ site on plasmid pBR322 and a cix site on P1 DNA. The junctions formed on the resulting co‐integrate can also serve as cix sites. This observation implies a potential evolutionary process to bring genes under the control of a biological switch acting by DNA inversion.

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Relationships between temperate phages Mu and P1

Cited by 57 publications

References 47 publications

Isolation of P1cinC(+) and P1cinC(-) mutants and detection of their polypeptides involved in host specificity.

Isolation of P1cinC(+) and P1cinC(-) mutants and detection of their polypeptides involved in host specificity.

Bacteriophage P1 Derivatives Unaffected in Their Growth by a Large Inversion or by IS Insertions at Various Locations

A site-specific, conservative recombination system carried by bacteriophage P1. Mapping the recombinase gene cin and the cross-over sites cix for the inversion of the C segment.

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