Relative Biological Effectiveness and Cell-Killing Efficacy of Continuous Low-Dose-Rate 125I Seeds on Prostate Carcinoma Cells In Vitro

Liao, Anyan; Wang, Junjie; Wang, Jidong; Zhuang, Hongqing; Zhao, Yong

doi:10.1177/1534735409357758

Cited by 15 publications

(16 citation statements)

References 40 publications

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“…Compared with SDR and FDR, CLDR caused severe total cell number decrease, due to enhanced cell death. The reported RBE of 125 I seed continuous low dose rate irradiation of different models and cell lines were among 1.2 and 2.1, and most of which were ranged from 1.3 to 1.5 [11,12,19-21]. Lehnert et al showed that 125 I irradiation caused increased RIF-1 cell death, and the RBE for 125 I compared with 60 Co gamma rays was 1.5 [21].…”

Section: Discussionmentioning

confidence: 99%

“…Wang et al reported that cell apoptosis of PANC-1 cell line increased after CLDR, with RBE for 125 I compared with 60 Co gamma rays being 1.39 [11]. Liao et al demonstrated that CLDR resulted in increased PC-3 cell apoptosis, with RBE for 125 I compared with 60 Co gamma rays being 1.41 [12]. In the present study, we have shown that the RBE for 125 I compared with 6 MV X- rays was 1.42, which is consistent with the previous data reported by our colleagues and others.…”

Section: Discussionmentioning

confidence: 99%

“…An in-house model for in vitro iodine-125 seed irradiation (shown in Figure 1) was developed for this study [10-12]. The model consisted of a 3-mm-thick polystyrene panel, with a lower seed plate layer and an upper-cell culture plate layer.…”

Section: Methodsmentioning

confidence: 99%

“…This model was thus validated by using thermoluminescent dosimetry (TLD) measurement and the absorbed doses for various exposure times, in various culture planes, were also measured and verified. The exposure times for delivering doses of 2, 4, 6 and 8Gy were 73.7, 154.6, 245.8 and 345.1 hours (i.e., 3.07, 6.44, 10.24 and 14.38 days), respectively [10-12]. Liner accelerators producing 6 MV X-ray beams were provided by the 306 th Hospital of the People’s Liberation Army (Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

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The different biological effects of single, fractionated and continuous low dose rate irradiation on CL187 colorectal cancer cells

Wang

et al. 2013

Radiat Oncol

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PurposeTo determine the biological effectiveness of single, fractionated and continuous low dose rate irradiation on the human colorectal cancer cell line CL187 in vitro and explore the cellular mechanisms.Materials and methodsThe CL187 cells were exposed to radiation of 6 MV X-ray at a high dose rate of 4Gy/min and 125I seed at a low dose rate of 2.77 cGy/h. Three groups were employed: single dose radiation group (SDR), fractionated dose radiation group (FDR) by 2Gy/f and continuous low dose rate radiation group (CLDR). Four radiation doses 2, 4, 6 and 8Gy were chosen and cells without irradiation as the control. The responses of CL187 cells to distinct modes of radiation were evaluated by the colony-forming assay, cell cycle progression as well as apoptosis analysis. In addition, we detected the expression patterns of DNA-PKcs, Ku70 and Ku80 by Western blotting.ResultsThe relative biological effect for 125I seeds compared with 6 MV X-ray was 1.42. 48 hrs after 4Gy irradiation, the difference between proportions of cells at G2/M phase of SDR and CLDR groups were statistically significant (p = 0.026), so as the FDR and CLDR groups (p = 0.005). 48 hrs after 4Gy irradiation, the early apoptotic rate of CLDR group was remarkably higher than SDR and FDR groups (CLDR vs. SDR, p = 0.001; CLDR vs. FDR, p = 0.02), whereas the late apoptotic rate of CLDR group increased significantly compared with SDR and FDR group (CLDR vs. SDR, p = 0.004; CLDR vs. FDR, p = 0.007). Moreover, DNA-PKcs and Ku70 expression levels in CLDR-treated cells decreased compared with SDR and FDR groups.ConclusionsCompared with the X-ray high dose rate irradiation, 125I seeds CLDR showed more effective induction of cell apoptosis and G2/M cell cycle arrest. Furthermore, 125I seeds CLDR could impair the DNA repair capability by down-regulating DNA-PKcs and Ku70 expression.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The different biological effects of single, fractionated and continuous low dose rate irradiation on CL187 colorectal cancer cells

Wang

et al. 2013

Radiat Oncol

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“…Because of its high precision and low complication rate, radioactive 125 I seed implantation has been widely applied in treatment of cancers, such as recurrent colorectal cancer [6,7], head and neck carcinoma and NPC [4,5]. Several studies have demonstrated that 125 I seed irradiation is more effective in inducing cell apoptosis in PANC-1 pancreatic [8] and CL187 colonic cells [9,10]. …”

Section: Introductionmentioning

confidence: 99%

Radioactive 125I Seed Inhibits the Cell Growth, Migration, and Invasion of Nasopharyngeal Carcinoma by Triggering DNA Damage and Inactivating VEGF-A/ERK Signaling

et al. 2013

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Although radiotherapy technology has progressed rapidly in the past decade, the inefficiency of radiation and cancer cell resistance mean that the 5-year survival rate of patients with nasopharyngeal carcinoma (NPC) is low. Radioactive 125I seed implantation has received increasing attention as a clinical treatment for cancers. Vascular endothelial growth factor-A (VEGF-A) is one of the most important members of the VEGF family and plays an important role in cell migration through the extracellular-signal-regulated kinase (ERK) pathway. Here we show that radioactive 125I seeds more effectively inhibit NPC cell growth through DNA damage and subsequent induction of apoptosis, compared with X-ray irradiation. Moreover, cell migration was effectively inhibited by 125I seed irradiation through VEGF-A/ERK inactivation. VEGF-A pretreatment significantly blocked 125I seed irradiation-induced inhibition of cell migration by recovering the levels of phosphorylated ERK (p-ERK) protein. Interestingly, in vivo study results confirmed that 125I seed irradiation was more effective in inhibiting tumor growth than X-ray irradiation. Taken together, these results suggest that radioactive 125I seeds exert novel anticancer activity by triggering DNA damage and inactivating VEGF-A/ERK signaling. Our finding provides evidence for the efficacy of 125I seeds for treating NPC patients, especially those with local recurrence.

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125I seeds inhibit proliferation and promote apoptosis in cholangiocarcinoma cells by regulating the AGR2-mediated p38 MAPK pathway

et al. 2022

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Relative Biological Effectiveness and Cell-Killing Efficacy of Continuous Low-Dose-Rate 125I Seeds on Prostate Carcinoma Cells In Vitro

Cited by 15 publications

References 40 publications

The different biological effects of single, fractionated and continuous low dose rate irradiation on CL187 colorectal cancer cells

The different biological effects of single, fractionated and continuous low dose rate irradiation on CL187 colorectal cancer cells

Radioactive 125I Seed Inhibits the Cell Growth, Migration, and Invasion of Nasopharyngeal Carcinoma by Triggering DNA Damage and Inactivating VEGF-A/ERK Signaling

125I seeds inhibit proliferation and promote apoptosis in cholangiocarcinoma cells by regulating the AGR2-mediated p38 MAPK pathway

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