Relative expression of matrix metalloproteinase-2 and -9 in synovial fluid from healthy calves and calves with experimentally induced septic arthritis

Francoz, David; Desrochers, André; Simard, Nathalie; Pierre, Yves Saint; Fecteau, Gilles; Latouche, Jean Sébastien; Fortin, Mado

doi:10.2460/ajvr.69.8.1022

Cited by 6 publications

(10 citation statements)

References 36 publications

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“…In cattle, higher activity in milk has also been reported after endotoxin-induced mastitis and in synovial fluid after an experimentally induced septic arthritis (Raulo et al, 2002;Francoz et al, 2008). Furthermore, MMP-9 can be covalently linked to Hp and released as MMP9-Hp complexes by neutrophils when their degranulation occurs.…”

Section: Other Biomarkers That Could Have Potential To Detect Sepsismentioning

confidence: 97%

Biomarkers of sepsis in pigs, horses and cattle: from acute phase proteins to procalcitonin

López-Martínez

Franco‐Martínez

Martínez-Subiela

et al. 2022

Anim. Health. Res. Rev.

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Sepsis is a complex clinical syndrome triggered by an inflammatory host response to an infection. It is usually complicated to detect and diagnose, and has severe consequences in human and veterinary health, especially when treatment is not started early. Therefore, efforts to detect sepsis accurately are needed. In addition, its proper diagnosis could reduce the misuse of antibiotics, which is essential fighting against antimicrobial resistance. This case is a particular issue in farm animals, as antibiotics have been traditionally given massively, but now they are becoming increasingly restricted. When sepsis is suspected in animals, the most frequently used biomarkers are acute phase proteins such as C-reactive protein, serum amyloid A and haptoglobin, but their concentrations can increase in other inflammatory conditions. In human patients, the most promising biomarkers to detect sepsis are currently procalcitonin and presepsin, and there is a wide range of other biomarkers under study. However, there is little information on the application of these biomarkers in veterinary species. This review aims to describe the general concepts of sepsis and the current knowledge about the biomarkers of sepsis in pigs, horses, and cattle and to discuss possible advances in the field.

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Section: Other Biomarkers That Could Have Potential To Detect Sepsismentioning

confidence: 97%

Biomarkers of sepsis in pigs, horses and cattle: from acute phase proteins to procalcitonin

López-Martínez

Franco‐Martínez

Martínez-Subiela

et al. 2022

Anim. Health. Res. Rev.

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“…Well-controlled bovine lameness induction models are scarce in the published literature. Models based on intraarticular inoculation of bacteria are difficult to standardize and control, and often cause intense and prolonged pain (Francoz et al, 2008). Amphotericin B, a polyene antibiotic, produces a controlled, mild to moderately severe synovitis-arthritis of short duration (Fahmy et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Analgesic efficacy of sodium salicylate in an amphotericin B-induced bovine synovitis-arthritis model

Kotschwar

Coetzee

Anderson

et al. 2009

Journal of Dairy Science

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This study examined the efficacy of sodium salicylate for providing analgesia in an amphotericin B-induced bovine synovitis-arthritis model using 10 male Holstein calves, 4 to 6 mo old and weighing approximately 250 kg. The study used a repeated measures partial crossover design with 2 phases, consisting of 3 treatment periods within each phase. Calves were blocked by body weight and randomly assigned to the sodium salicylate (50 mg/kg i.v.) or placebo group for phase 1. In period 1, lameness induction was simulated with a needle prick of the coronary band, followed by drug or placebo administration. At predetermined time points, serial blood samples for cortisol and salicylate concentrations, electrodermal activity measurements, heart rates, and pressure mat data were collected. Visual lameness scores were recorded by an observer blinded to treatments. In period 2, lameness was induced with injection of amphotericin B into the distal interphalangeal joint, followed by drug or placebo administration, with sample collection as described previously. In period 3, the drug or placebo was administered to the respective calves with sample collection. After a 10-d washout period, phase 2 was conducted with treatments crossed over between groups. Cortisol and salicylate samples were analyzed by competitive chemiluminescent immunoassay and fluorescence polarization immunoassay, respectively. The pharmacokinetic data were analyzed using compartmental analysis. Mean intravenous salicylate apparent volume of distribution was 0.2 +/- 0.005 L/kg, total body clearance was 4.3 +/- 0.2 mL/min.kg, and elimination half-life was 36.9 +/- 1.2 min. The repeated measures data were analyzed based on a univariate split-plot approach with a random effects-mixed model. Differences in stance phase duration and serum cortisol concentration values were seen both between periods and between treatment group x periods; differences in heart rate, contact surface area, and contact pressure values were seen between periods, suggesting that our lameness model was effective. No differences were seen between treatment groups. When analyzed by visual lameness score, differences were seen in heart rate, contact surface area, contact pressure, and cortisol concentrations. Area under the time-effect curves, determined by using the trapezoidal rule, had results similar to the repeated measures data, except for a difference in period for electrodermal activity. This amphotericin B-induced synovitis-arthritis model is a useful tool for studying changes associated with lameness in cattle. Sodium salicylate was not effective in providing analgesia after lameness.

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“…Arican et al found increased concentrations of MMP-9 activity in the synovial fluid of cows with septic arthritis in comparison with fluids from normal joints, using gelatin zymography and ELISA [2]. Two more animal studies confirmed the above data in horses and calves [7,9]. Two more animal studies confirmed the above data in horses and calves [7,9].…”

Section: Discussionmentioning

confidence: 79%

“…It is possible that pro-MMP-9, activated by bacterial acids, plays a crucial role in joint destruction [9]. It is possible that pro-MMP-9, activated by bacterial acids, plays a crucial role in joint destruction [9].…”

Section: Discussionmentioning

confidence: 99%

Expression levels of matrix metalloproteinase (MMP)-9 and its specific inhibitor TIMP-1, in septic and aseptic arthritis of the knee

Fotopoulos

Τzinia

Tzurbakis

et al. 2011

Knee Surg Sports Traumatol Arthrosc

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Relative expression of matrix metalloproteinase-2 and -9 in synovial fluid from healthy calves and calves with experimentally induced septic arthritis

Abstract: Results indicated that relative expression of MMP-9 and MMP-2 increased in synovial fluid from calves with experimentally induced septic arthritis, with relative expression remaining high for several days after infection.

Cited by 6 publications

References 36 publications

Biomarkers of sepsis in pigs, horses and cattle: from acute phase proteins to procalcitonin

Biomarkers of sepsis in pigs, horses and cattle: from acute phase proteins to procalcitonin

Analgesic efficacy of sodium salicylate in an amphotericin B-induced bovine synovitis-arthritis model

Expression levels of matrix metalloproteinase (MMP)-9 and its specific inhibitor TIMP-1, in septic and aseptic arthritis of the knee

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